公开(公告)号：US10088488B2

公开(公告)日：2018-10-02

申请号：US15441455

申请日：2017-02-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Roger Walker ,  Benedicte Jardin

IPC: C07K16/18 ,  G01N33/72 ,  G01N33/58

Abstract: Hemoglobin, its variants, and glycated forms of each are determined individually in a multiplex assay that permits correction of the measured level of HbA1c to account for glycated variants and other factors related to the inclusion of the variants in the sample. New antibodies that are particularly well adapted to the multiplex assay are also provided.

公开(公告)号：US20170307633A1

公开(公告)日：2017-10-26

申请号：US15617979

申请日：2017-06-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Roger Walker ,  Yabin Lu ,  Urvee Desai ,  Daming Shan

IPC: G01N33/68 ,  C07K14/415 ,  G01N33/564 ,  C12N9/10

CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06

Abstract: Methods of diagnosing celiac disease in a subject are provided. In some embodiments, the method comprises contacting a sample of bodily fluid from the subject with an antigen comprising a recombinant or synthetic deamidated gliadin protein, wherein the deamidated gliadin protein comprises a hexamer of peptides each having the sequence of SEQ ID NO:1; and detecting an antibody from the sample that specifically binds to the antigen, thereby diagnosing celiac disease in the subject.

公开(公告)号：US09702885B2

公开(公告)日：2017-07-11

申请号：US13692938

申请日：2012-12-03

Applicant: Bio Rad Laboratories, Inc.

Inventor： Roger Walker ,  Yabin Lu ,  Urvee Desai ,  Daming Shan

IPC: G01N33/68 ,  C12N9/10 ,  C07K14/415 ,  G01N33/564

CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

公开(公告)号：US20170176462A1

公开(公告)日：2017-06-22

申请号：US15441455

申请日：2017-02-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Roger Walker ,  Benedicte Jardin

IPC: G01N33/72 ,  C07K16/18

CPC classification number: G01N33/721 ,  C07K16/18 ,  C07K2317/34 ,  C07K2317/92 ,  G01N33/531 ,  G01N33/582 ,  G01N33/723 ,  Y10T436/101666 ,  Y10T436/25125

Abstract: Hemoglobin, its variants, and glycated forms of each are determined individually in a multiplex assay that permits correction of the measured level of HbA1c to account for glycated variants and other factors related to the inclusion of the variants in the sample. New antibodies that are particularly well adapted to the multiplex assay are also provided.

公开(公告)号：US20140323332A1

公开(公告)日：2014-10-30

申请号：US14261525

申请日：2014-04-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ravi Kaul ,  Weiming Zheng ,  Roger Walker

IPC: G01N33/576

CPC classification number: G01N33/5761 ,  G01N33/54326

Abstract: Provided herein are multiplex assays for determining whether an individual is or has been infected with Hepatitis B virus, and the stage of infection or resolution. In addition, the multiplex system can discriminate between vaccinated subjects and subjects susceptible to Hepatitis B infection.

Abstract translation: 本文提供用于确定个体是否已经感染乙型肝炎病毒的多重测定，以及感染或分辨的阶段。 此外，多重系统可以区分接种的受试者和易感乙型肝炎感染的受试者。