公开(公告)号：US20250129413A1

公开(公告)日：2025-04-24

申请号：US18983065

申请日：2024-12-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Sean CATER ,  Lucas FRENZ ,  Andrew KOHLWAY ,  Jennifer CHEW ,  Rob RAY ,  Ronald LEBOFSKY

IPC: C12Q1/6853 ,  C12N15/10 ,  C12Q1/6806

Abstract: Methods of assessing whether multiple differently-barcodes primers are in partitions are provided.

公开(公告)号：US20240229130A9

公开(公告)日：2024-07-11

申请号：US18381400

申请日：2023-10-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Ronald LEBOFSKY

IPC: C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/682

Abstract: Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.

公开(公告)号：US20240076658A1

公开(公告)日：2024-03-07

申请号：US18214941

申请日：2023-06-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI

IPC: C12N15/10

CPC classification number: C12N15/1096 ,  C12N15/1065

Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.

公开(公告)号：US20230323435A1

公开(公告)日：2023-10-12

申请号：US18185220

申请日：2023-03-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Vishal SHARMA ,  Ronald LEBOFSKY

IPC: C12Q1/6837 ,  C12N15/10

CPC classification number: C12Q1/6837 ,  C12N15/1065

Abstract: The methods allow for provision of a mixture of a plurality of beads, each bead linked to oligonucleotides, wherein the mixture can be treated as a bulk solution (prior to partitioning) to cleave a covalent bond linking the oligonucleotides to the beads while retaining a non-covalent linkage (via hybridization) between the beads and the oligonucleotides, allowing for distribution of the oligonucleotides and beads to partitions or 2D arrays prior to separation of the oligonucleotides from the beads, which occurs for example in the partitions.

公开(公告)号：US20220056435A1

公开(公告)日：2022-02-24

申请号：US17483032

申请日：2021-09-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeremy AGRESTI ,  Samantha COOPER ,  George KARLIN-NEUMANN ,  Nick HEREDIA ,  Ronald LEBOFSKY

IPC: C12N15/10 ,  C12Q1/6853

Abstract: Methods, compositions, and kits are provided for nucleic acid analysis, including single cell analysis.

公开(公告)号：US20210403989A1

公开(公告)日：2021-12-30

申请号：US17357618

申请日：2021-06-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Man CHENG

IPC: C12Q1/6834 ,  C12Q1/6869

Abstract: Barcoding composition and methods involving solid supports having sets of different oligonucleotides that can be decoded to the same identification sequence.

公开(公告)号：US20210130880A1

公开(公告)日：2021-05-06

申请号：US17087952

申请日：2020-11-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY

IPC: C12Q1/6834 ,  C12Q1/6855

Abstract: Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

公开(公告)号：US20210071173A1

公开(公告)日：2021-03-11

申请号：US17102111

申请日：2020-11-23

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe GmbH

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI

IPC: C12N15/10

Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.

公开(公告)号：US20200339977A1

公开(公告)日：2020-10-29

申请号：US16927690

申请日：2020-07-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6809

Abstract: Methods and compositions are provided for making and using uniquely tagged target nucleic acid molecules.

公开(公告)号：US20200071694A1

公开(公告)日：2020-03-05

申请号：US16687411

申请日：2019-11-18

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe GmbH

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI

IPC: C12N15/10

Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.