公开(公告)号：US20220056435A1

公开(公告)日：2022-02-24

申请号：US17483032

申请日：2021-09-23

申请人： Bio-Rad Laboratories, Inc.

发明人： Jeremy AGRESTI ,  Samantha COOPER ,  George KARLIN-NEUMANN ,  Nick HEREDIA ,  Ronald LEBOFSKY

IPC分类号： C12N15/10 ,  C12Q1/6853

摘要： Methods, compositions, and kits are provided for nucleic acid analysis, including single cell analysis.

公开(公告)号：US20190203274A1

公开(公告)日：2019-07-04

申请号：US16352600

申请日：2019-03-13

申请人： Bio-Rad Laboratories, Inc. ,  J. David Gladstone Institutes

发明人： Jennifer BERMAN ,  Samantha COOPER ,  George KARLIN-NEUMANN ,  Yuichiro MIYAOKA ,  Bruce CONKLIN ,  Josh SHINOFF

IPC分类号： C12Q1/6827 ,  C12N15/63 ,  C12Q1/6809 ,  C12N15/10

CPC分类号： C12Q1/6827 ,  C12N15/102 ,  C12N15/63 ,  C12Q1/6809 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/101

摘要： Methods, compositions, and kits are provided for quantification of genome editing.

公开(公告)号：US20190161793A1

公开(公告)日：2019-05-30

申请号：US16229657

申请日：2018-12-21

申请人： Bio-Rad Laboratories, Inc.

发明人： Dianna MAAR ,  Samantha COOPER ,  Wei YANG

IPC分类号： C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6848 ,  C12Q1/6827

摘要： Methods of detecting or quantifying short RNA or DNA molecules using split cycle amplification are provided.

公开(公告)号：US20160265041A1

公开(公告)日：2016-09-15

申请号：US15046297

申请日：2016-02-17

申请人： Bio-Rad Laboratories, Inc.

发明人： Dianna MAAR ,  Samantha COOPER ,  Wei YANG

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q2525/161 ,  C12Q2525/204 ,  C12Q2525/207 ,  C12Q2527/107 ,  C12Q2563/159 ,  C12Q2531/113 ,  C12Q2521/107 ,  C12Q2525/173 ,  C12Q2525/185

摘要： Methods of detecting or quantifying short RNA or DNA molecules using split cycle amplification are provided.

摘要翻译： 提供了使用分裂循环扩增检测或定量短RNA或DNA分子的方法。

公开(公告)号：US20220112544A1

公开(公告)日：2022-04-14

申请号：US17556118

申请日：2021-12-20

申请人： Bio-Rad Laboratories, Inc. ,  J. David Gladstone Institutes

发明人： Jennifer BERMAN ,  Samantha COOPER ,  George KARLIN-NEUMANN ,  Yuichiro MIYAOKA ,  Bruce CONKLIN ,  Josh SHINOFF

IPC分类号： C12Q1/6827 ,  C12Q1/6809 ,  C12N15/10 ,  C12N15/63

摘要： Methods, compositions, and kits are provided for quantification of genome editing.

公开(公告)号：US20220033883A1

公开(公告)日：2022-02-03

申请号：US17396148

申请日：2021-08-06

申请人： Bio-Rad Laboratories, Inc.

发明人： John F. REGAN ,  Dianna MAAR ,  Dawne N. SHELTON ,  Tina C. LEGLER ,  Samantha COOPER ,  Wei YANG ,  Eli A. HEFNER ,  Niels KLITGORD

IPC分类号： C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

摘要： Method of analysis for alleles of a target. In the method, a plurality of fluid volumes may be formed. Each fluid volume may contain a first primer pair to amplify a first allele of a target, a second primer pair to amplify a second allele of the target, a first reporter including a first photoluminophore and providing a primer of the first primer pair, and a second reporter including a second photoluminophore and providing a primer of the second primer pair. Each of the first and second reporters may include an oligomer having a quencher, and the oligomer may be configured to base-pair with the primer of the first primer pair and the primer of the second primer pair. The first and second alleles may be amplified using the first and second primer pairs. Photoluminescence may be detected. A level of each allele may be determined.

公开(公告)号：US20200270677A1

公开(公告)日：2020-08-27

申请号：US16834854

申请日：2020-03-30

申请人： Bio-Rad Laboratories, Inc.

发明人： John F. REGAN ,  Dianna MAAR ,  Dawne N. SHELTON ,  Tina C. LEGLER ,  Samantha COOPER ,  Wei YANG ,  Eli A. HEFNER ,  Niels KLITGORD

IPC分类号： C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

摘要： System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.