公开(公告)号：US20220259680A1

公开(公告)日：2022-08-18

申请号：US17670811

申请日：2022-02-14

Applicant: Bio-Rad Laboratories, Inc. ,  Biodesix, Inc.

Inventor： Dianna MAAR ,  Monica HERRERA ,  George KARLIN-NEUMANN ,  Josh SHINOFF ,  Audrey AUDETAT ,  Scott HUTTON ,  Hestia MELLERT ,  Leisa JACKSON ,  Gary PESTANO

IPC: C12Q1/6888

Abstract: Methods and compositions for characterizing a biological sample (e.g., comprising an infectious agent) from a subject are provided. Methods can include detecting linkage of nucleic acids that are linked in a viable cell or organism but that become degraded and thus unlinked in inviable cells or organisms and then characterizing the subject based on the quantity of linked and unlinked sequences.

公开(公告)号：US20220056435A1

公开(公告)日：2022-02-24

申请号：US17483032

申请日：2021-09-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeremy AGRESTI ,  Samantha COOPER ,  George KARLIN-NEUMANN ,  Nick HEREDIA ,  Ronald LEBOFSKY

IPC: C12N15/10 ,  C12Q1/6853

Abstract: Methods, compositions, and kits are provided for nucleic acid analysis, including single cell analysis.

公开(公告)号：US20190203274A1

公开(公告)日：2019-07-04

申请号：US16352600

申请日：2019-03-13

Applicant: Bio-Rad Laboratories, Inc. ,  J. David Gladstone Institutes

Inventor： Jennifer BERMAN ,  Samantha COOPER ,  George KARLIN-NEUMANN ,  Yuichiro MIYAOKA ,  Bruce CONKLIN ,  Josh SHINOFF

IPC: C12Q1/6827 ,  C12N15/63 ,  C12Q1/6809 ,  C12N15/10

CPC classification number: C12Q1/6827 ,  C12N15/102 ,  C12N15/63 ,  C12Q1/6809 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/101

Abstract: Methods, compositions, and kits are provided for quantification of genome editing.

公开(公告)号：US20220112544A1

公开(公告)日：2022-04-14

申请号：US17556118

申请日：2021-12-20

Applicant: Bio-Rad Laboratories, Inc. ,  J. David Gladstone Institutes

Inventor： Jennifer BERMAN ,  Samantha COOPER ,  George KARLIN-NEUMANN ,  Yuichiro MIYAOKA ,  Bruce CONKLIN ,  Josh SHINOFF

IPC: C12Q1/6827 ,  C12Q1/6809 ,  C12N15/10 ,  C12N15/63

Abstract: Methods, compositions, and kits are provided for quantification of genome editing.

公开(公告)号：US20180371525A1

公开(公告)日：2018-12-27

申请号：US16012610

申请日：2018-06-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI ,  George KARLIN-NEUMANN

IPC: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/682 ,  C12Q1/686 ,  C12Q1/6832 ,  C12Q1/6853

Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.

公开(公告)号：US20240093268A1

公开(公告)日：2024-03-21

申请号：US18381371

申请日：2023-10-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI ,  George KARLIN-NEUMANN

IPC: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/682 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/686

CPC classification number: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/682 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/686

Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.

公开(公告)号：US20210147925A1

公开(公告)日：2021-05-20

申请号：US17098006

申请日：2020-11-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： George KARLIN-NEUMANN ,  Douglas GREINER

IPC: C12Q1/6851 ,  C12Q1/6827 ,  C12Q1/6806 ,  C12Q1/6886

Abstract: Methods of analyzing a sample including cells and/or cell-free nuclei. In an exemplary method, partitions may be formed, with each partition including a portion of the same sample. Each partition of at least a subset of the partitions may contain only one of the cells/nuclei from the sample. Cells and/or cell-free nuclei from the sample may be lysed in the partitions. At least one amplification reaction may be performed for a target or set of targets in the partitions. Amplification data may be collected from the partitions in an exponential/linear phase of each amplification reaction. A copy number of the target or set of targets may be determined for individual partitions using the amplification data, to determine if either a duplication or deletion is present in all or a subset of the cells analyzed.