公开(公告)号：US10457890B2

公开(公告)日：2019-10-29

申请号：US15564275

申请日：2016-04-08

Applicant: DSM IP ASSETS B.V.

Inventor： Arjen Sein ,  Rene Marcel De Jong ,  Evert Tjeerd Van Rij ,  Willem Bijleveld ,  Maarten Hotse Wilbrink

IPC: C11B3/00 ,  C12N9/16 ,  C12P7/64 ,  C12P7/20

Abstract: The present invention relates to a polypeptide having phospholipase C activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. A process for degumming a vegetable oil comprising contacting a vegetable oil comprising phospholipids with a polypeptide having phospholipase C activity of the invention or a composition of the invention, wherein phospholipids are hydrolyzed into diacylglycerol and phosphate ester and/or phosphate, separating the phosphate ester and/or phosphate from the vegetable oil wherein a degummed vegetable oil is obtained.

公开(公告)号：US10327449B2

公开(公告)日：2019-06-25

申请号：US15716218

申请日：2017-09-26

Applicant: DSM IP ASSETS B.V.

Inventor： Rene Marcel De Jong ,  Hanna Elsabet Abbas ,  Evert Tjeerd Van Rij

IPC: C12N1/20 ,  A21D8/04 ,  C12N9/28 ,  A21D10/00

Abstract: A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue. Such a variant polypeptide may be used in the preparation of a baked product.

公开(公告)号：US10316305B2

公开(公告)日：2019-06-11

申请号：US15962058

申请日：2018-04-25

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter Los ,  Rene Marcel De Jong ,  Maaike Appeldoorn

IPC: C12N9/24 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14

Abstract: The invention relates to a polypeptide having hemicellulase activity. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

公开(公告)号：US10253305B2

公开(公告)日：2019-04-09

申请号：US15782528

申请日：2017-10-12

Applicant: DSM IP ASSETS B.V.

Inventor： Petrus Jacobus Theodorus Dekker ,  Rene Marcel De Jong ,  Cornelis Marinus Muijlwijk

IPC: C12N9/64 ,  A23C19/032 ,  A23C19/068 ,  A23C19/072

Abstract: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.

公开(公告)号：US09301533B2

公开(公告)日：2016-04-05

申请号：US13842344

申请日：2013-03-15

Applicant: DSM IP ASSETS B.V.

Inventor： Rene Marcel De Jong ,  Hanna Elisabet Abbas

IPC: C12N9/28 ,  C07H21/04 ,  A21D8/04

CPC classification number: A21D8/042 ,  C12N9/2417 ,  C12Y302/01001

Abstract: A variant polypeptide having alpha-amylase activity is disclosed. The variant polypeptide has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue with reference to SEQ ID NO: 2. The variant polypeptide has one or more altered properties as compared with a reference polypeptide having alpha-amylase activity. Such a variant polypeptide may be used in the preparation of a baked product.

Abstract translation: 公开了具有α-淀粉酶活性的变体多肽。 所述变体多肽具有当与包含SEQ ID NO：2所示的序列的α-淀粉酶比对时的氨基酸序列包含参考SEQ ID NO：2的氨基酸残基的至少一个取代。所述变体 与具有α-淀粉酶活性的参考多肽相比，多肽具有一个或多个改变的性质。 这样的变体多肽可用于制备焙烤产品。