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公开(公告)号:US06867196B1
公开(公告)日:2005-03-15
申请号:US09330909
申请日:1999-06-11
申请人: Jon A. Wolff , Hans Herweijer , Larry F. Whitesell , Matthew R. Wolff , Sean D. Monahan , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人: Jon A. Wolff , Hans Herweijer , Larry F. Whitesell , Matthew R. Wolff , Sean D. Monahan , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
CPC分类号: A61K48/0008 , A61K48/0075
摘要: A process for delivering a nucleic acid to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a nucleic acid to a blood vessel, which subsequently delivers the nucleic acid to the cardiac tissue cell. The nucleic acid can be DNA or RNA or plasmid DNA or viral. This process is for purposes of gene therapy, and research.
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公开(公告)号:US07045356B2
公开(公告)日:2006-05-16
申请号:US10795679
申请日:2004-03-08
申请人: Vladimir Trubetskoy , Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , So Chun Wong , Jason Klein
发明人: Vladimir Trubetskoy , Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , So Chun Wong , Jason Klein
IPC分类号: C12N15/88
CPC分类号: A61K48/0008
摘要: We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
摘要翻译: 我们描述了一种产生多层颗粒的方法,包括将聚合物与带相反电荷的聚合物缩合形成颗粒,并依次向形成至少三层聚合物的颗粒中加入带相反电荷的聚合物。 该方法用于形成用于将生物活性化合物递送至细胞的组合物。
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公开(公告)号:US07101995B2
公开(公告)日:2006-09-05
申请号:US10157674
申请日:2002-05-28
申请人: David L. Lewis , James E. Hagstrom , Hans Herweijer , Aaron G. Loomis , Sean D. Monahan , Jon A. Wolff
发明人: David L. Lewis , James E. Hagstrom , Hans Herweijer , Aaron G. Loomis , Sean D. Monahan , Jon A. Wolff
CPC分类号: C12N15/1137 , A61K31/58 , A61K31/74 , A61K38/00 , A61K48/00 , C12N15/111 , C12N15/88 , C12N2310/14 , C12N2320/32 , C12N2710/24111 , C12Y206/01002 , A61K2300/00
摘要: Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.
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公开(公告)号:US07148205B2
公开(公告)日:2006-12-12
申请号:US10600290
申请日:2003-06-20
申请人: Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人: Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
CPC分类号: C12N15/87 , A61K31/70 , A61K47/59 , A61K47/593 , A61K47/645 , A61K48/00 , A61K48/0083 , C07H21/00 , C12N15/111 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/3233 , C12N2320/32 , A61K2300/00
摘要: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
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公开(公告)号:US06379966B2
公开(公告)日:2002-04-30
申请号:US09450315
申请日:1999-11-29
申请人: Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人: Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
IPC分类号: C12N1585
CPC分类号: A61K48/0041 , A61K48/0008 , A61K48/0083
摘要: Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
摘要翻译: 公开了用于在细胞中提供核酸表达的复合物。 将多核苷酸和聚合物混合在一起形成复合物,其中络合物的ζ电位不是正的。 然后将复合物递送到其中表达核酸的细胞。
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公开(公告)号:US07022525B2
公开(公告)日:2006-04-04
申请号:US10755785
申请日:2004-01-12
申请人: Vladimir Trubetskoy , Jon A. Wolff , Paul M. Slattum , Lisa Hanson , Vladimir G. Budker , James E. Hagstrom
发明人: Vladimir Trubetskoy , Jon A. Wolff , Paul M. Slattum , Lisa Hanson , Vladimir G. Budker , James E. Hagstrom
IPC分类号: C12N15/09 , C12N15/00 , C12N15/11 , A61K48/00 , A61K39/395
CPC分类号: C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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公开(公告)号:US06818626B1
公开(公告)日:2004-11-16
申请号:US09234606
申请日:1999-01-21
申请人: Jon A. Wolff , James E. Hagstrom , Sean D. Monahan , Vladimir Budker , Vladimir Trubetskoy , Paul M. Slattum
发明人: Jon A. Wolff , James E. Hagstrom , Sean D. Monahan , Vladimir Budker , Vladimir Trubetskoy , Paul M. Slattum
IPC分类号: A61K4800
CPC分类号: A61K48/0025 , A61K48/00 , C12N15/87
摘要: Chelator containing compounds are utilized in the delivery of molecules, polymers, nucleic acids and genes to animal cells. At least one chelator such as crown ether is attached to a polymer and then associated with another polymer such as DNA. An ion is then added to the mixture thereby forming condensed DNA. In condensed form and in complex with the chelator, DNA can be delivered to a cell.
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公开(公告)号:US06897068B2
公开(公告)日:2005-05-24
申请号:US10085378
申请日:2002-02-27
申请人: Sean D. Monahan , Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , DAvid B. Rozema , Paul M. Slattum
发明人: Sean D. Monahan , Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , DAvid B. Rozema , Paul M. Slattum
CPC分类号: A61K48/0008 , A61K48/0041 , A61K48/0083
摘要: Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
摘要翻译: 公开了用于在细胞中提供核酸表达的复合物。 将多核苷酸和聚合物混合在一起形成复合物,其中络合物的ζ电位不是正的。 然后将复合物递送到其中表达核酸的细胞。
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公开(公告)号:US06627616B2
公开(公告)日:2003-09-30
申请号:US09447966
申请日:1999-11-23
申请人: Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人: Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
IPC分类号: C12N1500
CPC分类号: C12N15/113 , A61K31/70 , A61K47/58 , A61K47/59 , A61K47/593 , A61K47/62 , A61K47/6455 , A61K48/00 , A61K48/0008 , A61K48/0041 , A61K48/0083 , C07H21/00 , C12N15/87 , C12N2320/32 , A61K2300/00
摘要: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
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30.
公开(公告)号:US07816337B2
公开(公告)日:2010-10-19
申请号:US10780484
申请日:2004-02-17
CPC分类号: C12N15/111 , A61K47/58 , A61K48/00 , A61K48/0041 , C12N15/87 , C12N2310/351 , C12N2320/32
摘要: Described is a process for delivering a biologically active compound to a cell by reversibly linking the compound to a membrane active polymer. In particular, polymer-polynucleotide conjugates are described. Methods for reversibly modifying the polymers to decrease cellular toxicity and improve efficacy are provided.
摘要翻译: 描述了通过可逆地将化合物与膜活性聚合物连接而将生物活性化合物递送至细胞的方法。 特别地,描述了聚合物 - 多核苷酸缀合物。 提供了可逆修饰聚合物以降低细胞毒性并提高功效的方法。
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