公开(公告)号：US20210139884A1

公开(公告)日：2021-05-13

申请号：US17120030

申请日：2020-12-11

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Matthew KELLINGER ,  Molly HE ,  Tyler LOPEZ

IPC: C12N15/10 ,  C12N9/12

Abstract: Provided herein are compositions and methods for the incorporation of unnatural nucleotides using mutant polymerases, such as reverse transcriptases. Further provided herein are methods of detection and sequencing of polynucleotide sequences. In some aspects, the compositions and methods are used enhance the efficiency and speed of detecting nucleotide bases. The methods and compositions described herein may further reduce time, cost, or scale of devices for next generation sequencing platforms.

公开(公告)号：US20200182866A1

公开(公告)日：2020-06-11

申请号：US16739007

申请日：2020-01-09

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Sinan ARSLAN ,  Chunhong ZHOU ,  Molly Min HE ,  Matthew KELLINGER ,  Adeline Huizhen MAH ,  Michael PREVITE ,  Lei SUN

IPC: G01N33/543 ,  C12Q1/6844 ,  G01N21/64

Abstract: Improved system and method are described that utilize surfaces with low non-specific binding supports and formulations for performing solid-phase nucleic acid hybridization and amplification. The system and method described herein provide improved performance for nucleic acid detection and other applications.

公开(公告)号：US20240191225A1

公开(公告)日：2024-06-13

申请号：US18465687

申请日：2023-09-12

Applicant: Element Biosciences, Inc.

Inventor： Junhua ZHAO ,  Xiaodong QI ,  Kelly BLEASE ,  Hua YU ,  Matthew KELLINGER

IPC: C12N15/10

CPC classification number: C12N15/1093

Abstract: The present disclosure provides compositions comprising nucleic acid double-stranded splint adaptors, including kits, and methods that employ the double-stranded splint adaptors. The double-stranded splint adaptors (200) can be used in a one-pot, multi-enzyme reaction to introduce one or more new adaptor sequences into a library molecule. The double-stranded splint adaptor (200) comprises a first splint strand (long splint strand (300)) and a second splint strand (short splint strand (400)), where the first and second splint strands are hybridized together to form the double-stranded splint adaptor (200) having a double-stranded region and two flanking single-stranded regions. The second splint strand (400) carries the new adaptor sequence(s) to be introduced, such as for example a universal binding sequence, an index sequence and/or a random sequence.

公开(公告)号：US20230392201A1

公开(公告)日：2023-12-07

申请号：US18330279

申请日：2023-06-06

Applicant: Element Biosciences, Inc.

Inventor： James A. STAPLETON ,  Timothy WHITEHEAD ,  Michael PREVITE ,  Molly HE ,  Tuval BEN-YEHEZKEL ,  Matthew KELLINGER ,  Kyle METCALFE

IPC: C12Q1/6869 ,  C12Q1/686 ,  C12N15/10 ,  C12Q1/6813

CPC classification number: C12Q1/6869 ,  C12Q1/686 ,  C12N15/1065 ,  C12Q1/6813

Abstract: The disclosure relates to methods for obtaining nucleic acid sequence information by constructing a nucleic acid library and reconstructing longer nucleic acid sequences by assembling a series of shorter nucleic acid sequences.

公开(公告)号：US20230296593A1

公开(公告)日：2023-09-21

申请号：US18202247

申请日：2023-05-25

Applicant: Element Biosciences, Inc.

Inventor： Michael PREVITE ,  Molly HE ,  Junhua ZHAO ,  Hui Zhen MAH ,  Chunhong ZHOU ,  Sinan ARSLAN ,  Matthew KELLINGER ,  Lorenzo BERTI ,  Steve Xiangling CHEN

IPC: G01N33/53 ,  C12Q1/6874 ,  G01N33/58

CPC classification number: G01N33/5308 ,  C12Q1/6874 ,  G01N33/582

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20230265402A1

公开(公告)日：2023-08-24

申请号：US18160952

申请日：2023-01-27

Applicant: Element Biosciences, Inc.

Inventor： Jendrik HENTSHCEL ,  Tyler LOPEZ ,  Michael KLEIN ,  Virginia SAADE ,  Matthew KELLINGER ,  Mark AMBROSO

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Y207/07007

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US20210373000A1

公开(公告)日：2021-12-02

申请号：US17398315

申请日：2021-08-10

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG ,  Tyler LOPEZ

IPC: G01N33/53 ,  G01N33/58 ,  C12Q1/6874

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20210123911A1

公开(公告)日：2021-04-29

申请号：US17063608

申请日：2020-10-05

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG ,  Tyler LOPEZ

IPC: G01N33/53 ,  C12Q1/6874 ,  G01N33/58

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20210121882A1

公开(公告)日：2021-04-29

申请号：US17082445

申请日：2020-10-28

Applicant: Element Biosciences, Inc.

Inventor： Minghao GUO ,  Leon Zilun ZHANG ,  Chunhong ZHOU ,  Matthew KELLINGER ,  Michael PREVITE

IPC: B01L3/00

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US20210040534A1

公开(公告)日：2021-02-11

申请号：US17070796

申请日：2020-10-14

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Chunhong ZHOU ,  Sinan ARSLAN ,  Molly Min HE ,  Matthew KELLINGER ,  Adeline Huizhen MAH ,  Michael PREVITE ,  Lei SUN

IPC: C12Q1/682 ,  C12Q1/6869 ,  C12Q1/6867

Abstract: Methods and formulations for preparing low non-specific binding surfaces are described, and the prepared surface can provide improved performance for nucleic acid detection and base calling applications. The surface provides more accurate nucleic acid detection, enhanced contrast to noise ratio, and better data collection.