公开(公告)号：US20110258715A1

公开(公告)日：2011-10-20

申请号：US13059941

申请日：2009-08-21

申请人： Hiromitsu Nakauchi ,  Toshihiro Kobayashi ,  Tomoyuki Yamaguchi ,  Sanae Hamanaka

发明人： Hiromitsu Nakauchi ,  Toshihiro Kobayashi ,  Tomoyuki Yamaguchi ,  Sanae Hamanaka

IPC分类号： A01K67/027 ,  A01K67/00 ,  C12N15/87

CPC分类号： A01K67/0271 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/025 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3895

摘要： It is revealed that an organ such as pancreas can be regenerated by utilizing a fact that the deficiency of an organ is complemented by injecting an induced pluripotent stem cell (iPS cell) into a developed blastocyst in a blastocyst complementation method. Thus, the present invention has solved the above-described object. This provides a method for producing a target organ, using an iPS cell, in a living body of a non-human mammal having an abnormality associated with a lack of development of the target organ in a development stage, the target organ produced being derived from a different individual mammal that is an individual different from the non-human mammal.

摘要翻译： 通过利用囊胚互补方法将发生的多能干细胞（iPS细胞）注射到发育的囊胚中，通过利用器官的不足补充了这一事实，能够再生胰脏等器官。 因此，本发明解决了上述目的。 这提供了使用iPS细胞在发育阶段中与目标器官缺乏发育相关的异常的非人哺乳动物的活体中产生靶器官的方法，所产生的靶器官来自 作为与非人哺乳动物不同的个体的不同个体哺乳动物。

公开(公告)号：US20060177932A1

公开(公告)日：2006-08-10

申请号：US10534386

申请日：2003-10-23

申请人： Hiromitsu Nakauchi ,  Atsushi Iwama

发明人： Hiromitsu Nakauchi ,  Atsushi Iwama

IPC分类号： A61K38/17 ,  C12N15/867 ,  C12N5/08

CPC分类号： C12N5/0647 ,  C07K14/4705 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/26

摘要： The present invention relates to a method and substance for maintaining the pluripotency and self-replication ability of a stem cell, such as a hematopoietic stem cell, while keeping it undifferentiated. Specifically, the present invention provides a composition for maintaining the expansion or pluripotency of a stem cell, comprising active STAT5, and a method using the same. STAT5 may be in the form of a protein or a nucleic acid. The composition may contain a cellular physiologically active substance (e.g., SCF, TPO, Flt-3L, etc.). The present invention also relates to a cell, a tissue and an organ prepared from the stem cell.

摘要翻译： 本发明涉及一种保持干细胞如造血干细胞的多能性和自我复制能力的方法和物质，同时保持其未分化。 具体地，本发明提供了用于维持包含活性STAT5的干细胞的扩增或多能性的组合物及其使用方法。 STAT5可以是蛋白质或核酸的形式。 组合物可以含有细胞生理活性物质（例如SCF，TPO，Flt-3L等）。 本发明还涉及从干细胞制备的细胞，组织和器官。

公开(公告)号：US20050221480A1

公开(公告)日：2005-10-06

申请号：US11075065

申请日：2005-03-03

申请人： Hiromitsu Nakauchi ,  Atsushi Suzuki ,  Hideki Taniguchi ,  Katashi Fukao

发明人： Hiromitsu Nakauchi ,  Atsushi Suzuki ,  Hideki Taniguchi ,  Katashi Fukao

IPC分类号： A61K35/12 ,  C12N5/071 ,  C12N5/074 ,  C12N5/08

CPC分类号： C12N5/0676 ,  A61K35/12 ,  C12N5/0672 ,  C12N2506/02 ,  C12N2506/14 ,  Y02A50/423 ,  Y02A50/463

摘要： The present invention provides clonal pluripotent hepatic stem cells using flow cytometry and in vitro single-cell-based assays. These cells possess multilineage differentiation potential and self-renewing capability. These cells could be clonally propagated in culture, where they continuously produced hepatocytes and cholangiocytes as descendants while maintaining primitive stem cells. When cells that expanded in vitro were transplanted into recipient animals, they morphologically and functionally differentiated into hepatocytes and cholangiocytes, with reconstitution of hepatocyte and bile duct structures. Furthermore, these cells differentiated into pancreatic ductal and acinar cells or intestinal epithelial cells when transplanted into pancreas or duodenal wall. These data indicate that self-renewing multipotent stem cells persist in the developing mouse liver and that such cells can be induced to become cells of other organs of endodermal origin under appropriate microenvironment. Manipulation of hepatic stem cells may provide new insight into therapies for diseases of the digestive system.

摘要翻译： 本发明使用流式细胞术和体外基于单细胞的测定法提供克隆多能肝干细胞。 这些细胞具有多谱分化潜能和自我更新能力。 这些细胞可以在培养物中克隆繁殖，在那里它们连续产生肝细胞和胆管细胞作为后代，同时保持原始干细胞。 当将体外扩增的细胞移植到受体动物中时，其形态和功能上分化为肝细胞和胆管细胞，重建肝细胞和胆管结构。 此外，当移植到胰腺或十二指肠壁时，这些细胞分化为胰腺导管和腺泡细胞或肠上皮细胞。 这些数据表明，自我更新的多潜能干细胞持续存在于发育中的小鼠肝脏中，并且在合适的微环境下，可以诱导这样的细胞成为内胚层起源的其它器官的细胞。 肝干细胞的操作可以为消化系统疾病的治疗提供新的见解。