公开(公告)号：US20180251735A1

公开(公告)日：2018-09-06

申请号：US15905982

申请日：2018-02-27

申请人： Elixirgen, LLC

发明人： Minoru S.H. Ko

IPC分类号： C12N5/074 ,  C12N15/85

CPC分类号： C12N5/0696 ,  C07K14/00 ,  C12N5/0619 ,  C12N5/0647 ,  C12N5/0658 ,  C12N5/067 ,  C12N15/113 ,  C12N15/85 ,  C12N15/86 ,  C12N2015/8518 ,  C12N2310/14 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2506/08 ,  C12N2506/1323 ,  C12N2506/14 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2523/00 ,  C12N2760/18741 ,  C12N2760/18843 ,  C12N2800/107

摘要： In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being stable and highly efficient. The use of complicated culture steps is a large problem. In addition, there are also large problems in, for example, that the speed of cell differentiation is low, and hence long-period culture is required, and that the differentiation efficiency is low, and hence it is difficult to obtain a sufficient number of required cells. A method of inducing differentiation into a desired cell type, which induces differentiation within a short period of time and with high efficiency by the use of a Sendai virus vector capable of expressing a transcription factor, and as required, the use of a pluripotent stem cell in which an expression amount of a POU5F1 protein has been substantially removed or reduced, is provided.

公开(公告)号：US20170369840A1

公开(公告)日：2017-12-28

申请号：US15651910

申请日：2017-07-17

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Marius Wernig ,  Thomas C. Sudhof ,  Thomas Vierbuchen ,  Austin Ostermeier ,  Zhiping Pang

IPC分类号： C12N5/0793 ,  C12N5/079 ,  G01N33/50 ,  C12N5/0797

CPC分类号： C12N5/0619 ,  C12N5/0622 ,  C12N5/0623 ,  C12N2501/60 ,  C12N2506/02 ,  C12N2506/1307 ,  C12N2506/14 ,  C12N2506/45 ,  C12N2510/00 ,  G01N33/5058

摘要： Methods, compositions and kits for producing functional neurons, astroctyes, oligodendrocytes and progenitor cells thereof are provided. These methods, compositions and kits find use in producing neurons, astrocytes, oligodendrocytes, and progenitor cells thereof for transplantation, for experimental evaluation, as a source of lineage- and cell-specific products, and the like, for example for use in treating human disorders of the CNS. Also provided are methods, compositions and kits for screening candidate agents for activity in converting cells into neuronal cells, astrocytes, oligodendrocytes, and progenitor cells thereof.

公开(公告)号：US09102913B2

公开(公告)日：2015-08-11

申请号：US13807260

申请日：2011-07-01

申请人： Marsha Lynn Roach ,  Richard Harold Malavarca ,  Yunfang Wang ,  Lola Cynthia McAdams Reid

发明人： Marsha Lynn Roach ,  Richard Harold Malavarca ,  Yunfang Wang ,  Lola Cynthia McAdams Reid

IPC分类号： A61K35/12 ,  C12N5/00 ,  A61L27/36 ,  C12N5/071

CPC分类号： C12N5/0068 ,  A61L27/24 ,  A61L27/3604 ,  A61L27/3683 ,  A61L27/3691 ,  A61L2430/28 ,  C12N5/067 ,  C12N5/0671 ,  C12N5/0693 ,  C12N7/00 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2501/20 ,  C12N2501/998 ,  C12N2506/14 ,  C12N2533/54 ,  C12N2533/90

摘要： The present invention provides biomatrix scaffolds for industrial scale dispersal.

摘要翻译： 本发明提供了用于工业规模扩散的生物基质支架。

公开(公告)号：US08883478B2

公开(公告)日：2014-11-11

申请号：US12709070

申请日：2010-02-19

申请人： Qiyong Peter Liu ,  Henrik Clausen

发明人： Qiyong Peter Liu ,  Henrik Clausen

IPC分类号： C12Q1/34 ,  C12N9/40 ,  C12P21/00 ,  C07K14/00

CPC分类号： C12N5/0654 ,  A61K35/32 ,  A61K35/35 ,  A61L27/3687 ,  A61L27/3695 ,  C12N5/0652 ,  C12N9/2465 ,  C12N2501/73 ,  C12N2506/09 ,  C12N2506/13 ,  C12N2506/1315 ,  C12N2506/14 ,  C12N2506/25 ,  C12Y302/01022

摘要： This invention relates to novel α-galactosidases for the enzymatic removal of the immunodominant monosaccharides on blood products and tissues. Specifically this invention provides a novel family of α3 glycosidases, used for the enzymatic removal of type B antigens from blood group B and AB reactive blood products, and the Galili antigen from non-human animal tissues, thereby converting these to non-immunogenic cells and tissues suitable for transplantation.

摘要翻译： 本发明涉及用于在血液制品和组织上酶促去除免疫优势单糖的新型α-半乳糖苷酶。 具体地说，本发明提供了一种新型的α3糖苷酶家族，用于从血型B和AB反应性血液制品中酶切除B型抗原，从非人动物组织中提取Galectin抗原，从而将其转化为非免疫原性细胞， 适合移植的组织。

公开(公告)号：US20030219894A1

公开(公告)日：2003-11-27

申请号：US10413358

申请日：2003-04-15

申请人： JCR Pharmaceuticals Co., Ltd. ,  Susumu SEINO

发明人： Susumu Seino ,  Nobuko Ishizuka ,  Masaaki Okuno

IPC分类号： C12N005/08 ,  C12N005/06

CPC分类号： C12N5/0676 ,  C12N2500/38 ,  C12N2501/60 ,  C12N2506/14 ,  C12N2510/00

摘要： What is described is a method for preparation of insulin-producing cells from non-insulin-producing cells. Mammalian fetal hepatocytes or hepatic progenitor cells are used ad the non-insulin-producing cells, and the method comprises culturing the mammalian fetal hepatocytes or the hepatic progenitor cells with 1-50 mmol/L of nicotinamide and concurrently bringing about expression of the PDX-1 gene or the NeuroD gene in the mammalian fetal hepatocytes.

摘要翻译： 描述的是从非胰岛素生产细胞制备胰岛素生成细胞的方法。 使用哺乳动物胎儿肝细胞或肝祖细胞和非胰岛素产生细胞，并且该方法包括用1-50mmol / L的烟酰胺培养哺乳动物胎儿肝细胞或肝祖细胞，同时使PDX- 1基因或哺乳动物胎儿肝细胞中的NeuroD基因。

公开(公告)号：US20030211603A1

公开(公告)日：2003-11-13

申请号：US10344680

申请日：2003-02-12

发明人： David J. Earp ,  Melissa K. Carpenter ,  Joseph D. Gold ,  Jane S. Lebkowski ,  J. Michael Schiff

IPC分类号： C12N005/08

CPC分类号： C12N5/067 ,  A61K35/12 ,  C12N5/0618 ,  C12N2502/02 ,  C12N2502/03 ,  C12N2503/00 ,  C12N2506/08 ,  C12N2506/11 ,  C12N2506/1353 ,  C12N2506/14

摘要： Described in this disclosure is a new process whereby cells of one tissue type can be reprogrammed to produce cells of a different tissue type. Cells from a human donor are reprogrammed by culturing adjacent to primate pluripotent stem cells (in an undifferentiated or newly differentiated state) or in an environment supplemented by components taken from pPS cells. Simultaneously or in a subsequent step, the donor cells can be treated in a manner that enhances differentiation towards a different tissue type. In this manner, patients in need of tissue regeneration can be treated with cells differentiated and reprogrammed from their own autologous cell donation.

摘要翻译： 在本公开中描述的是一种新方法，其中一种组织类型的细胞可以被重新编程以产生不同组织类型的细胞。 通过与灵长类多能干细胞（未分化或新分化状态）相邻的环境或在从由pPS细胞获取的组分补充的环境中培养来重新编程来自人供体的细胞。 同时或在随后的步骤中，供体细胞可以以增强朝不同组织类型分化的方式进行治疗。 以这种方式，需要组织再生的患者可以用从其自身的自体细胞捐献分化和重新编程的细胞来治疗。

公开(公告)号：US20240141300A1

公开(公告)日：2024-05-02

申请号：US17773080

申请日：2020-10-28

申请人： UNICYTE ISLET AG

发明人： VICTOR MANUEL NAVARRO TABLEROS ,  Maria Felice BRIZZI ,  Yonatan GOMEZ ,  Giovanni CAMUSSI

IPC分类号： C12N5/071 ,  A61K35/39

CPC分类号： C12N5/0677 ,  A61K35/39 ,  C12N2501/998 ,  C12N2503/04 ,  C12N2506/14

摘要： A method of preparing pancreatic islet-like cell structures having a unique combination of high viability, morphological and functional features that make the pancreatic islet-like cell structures particularly suitable for use in both clinical and drug screening application is provided. Pancreatic islet-like cell structures obtained from the method are also provided.

公开(公告)号：US20240060050A1

公开(公告)日：2024-02-22

申请号：US18469214

申请日：2023-09-18

申请人： Iowa State University Research Foundation, Inc.

发明人： Jonathan P. Mochel ,  Karin Allenspach Jorn ,  Vojtech Gabriel ,  Christopher Zdyrski

IPC分类号： C12N5/071

CPC分类号： C12N5/0671 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/39 ,  C12N2501/42 ,  C12N2503/04 ,  C12N2506/14 ,  C12N2510/00 ,  C12N2513/00

摘要： The present invention relates to hepatic organoids and uses thereof. The compositions include models for the study of developmental biology of the liver and other epithelial tissues, drug discovery and toxicity screening, infectious disease biology of various infectious agents, and personalized medicines. Methods for seeding canine intestinal organoids, maintaining an organoid monolayer, and monitoring monolayer integrity are provided. Methods and systems for culturing, freezing, and recovering the frozen organoid cells are also provided. The hepatic organoids may also be used to treat a subject in need, or for identifying a preferred therapeutic agent.

公开(公告)号：US20230272347A1

公开(公告)日：2023-08-31

申请号：US18100195

申请日：2023-01-23

申请人： Koninklijke Nederlandse Akademie Van Wetenschappen

发明人： Johannes Carolus Clevers ,  Helmuth Gehart

IPC分类号： C12N5/071 ,  A61K35/407 ,  G01N33/50

CPC分类号： C12N5/0672 ,  A61K35/407 ,  C12N5/0671 ,  G01N33/5088 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/345 ,  C12N2501/39 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2501/805 ,  C12N2501/999 ,  C12N2506/14

摘要： The invention relates to differentiation methods for progenitor cells, e.g. mammalian epithelial stem cells, differentiation media for use in said methods, organoids and cells obtainable by said methods and uses, including therapeutic uses, thereof.

公开(公告)号：US20160376557A1

公开(公告)日：2016-12-29

申请号：US15125596

申请日：2015-03-19

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  Universite Paris - SUD ,  Université de Rennes ,  Université Paris Descartes

发明人： Anne DUBART KUPPERSCHMITT ,  Anne WEBER BENAROUS ,  Anne CORLU ,  Noushin DIANAT

IPC分类号： C12N5/071 ,  A61K35/407

CPC分类号： C12N5/0679 ,  A61K35/407 ,  C12N5/0672 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/02 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/2306 ,  C12N2501/305 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/14 ,  C12N2506/45 ,  C12N2533/54

摘要： The invention relates to a method for inducing human cholangiocyte differentiation of progenitor cells called hepatoblasts. More specifically, the invention relates to a method for differentiating hepatoblasts to cholangiocytes by culturing said hepatoblasts with a particular medium having interleukin-6 (IL-6) activity. The differentiation method can specifically induce cholangiocyte differentiation from hepatoblasts, and the human cholangiocytes differentiated according to the invention may be useful for drug discovery for treatment of cholangiopathies and bioengineered livers.

摘要翻译： 本发明涉及诱导称为成肝细胞的祖细胞的人胆管细胞分化的方法。 更具体地，本发明涉及通过用具有白细胞介素-6（IL-6）活性的特定培养基培养所述成肝细胞来将成肝细胞与胆管细胞分化的方法。 分化方法可以特异性诱导来自成肝细胞的胆管细胞分化，根据本发明分化的人胆管细胞可用于治疗胆管病和生物工程化肝的药物发现。