公开(公告)号：US20200308562A1

公开(公告)日：2020-10-01

申请号：US16827639

申请日：2020-03-23

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani

IPC: C12N9/22 ,  C12Q1/44 ,  C12N15/85

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US11566241B2

公开(公告)日：2023-01-31

申请号：US17492435

申请日：2021-10-01

Applicant: Inscripta, Inc.

Inventor： Andrea Halweg-Edwards ,  Thomas Hraha ,  Krishna Yerramsetty ,  Shea Lambert ,  Miles Gander ,  Matthew David Estes ,  Chad Douglas Sanada ,  Isaac David Wagner ,  Paul Hardenbol

IPC: C12N15/10 ,  G16B35/20 ,  G16B40/20 ,  G16B35/10

Abstract: Disclosed systems and methods relate to predicting the relative representation of genomic variants in an edited cell population, based on the editing cassette design representation in an editing cassette design library used to generate the edited cell population. A library of editing cassette designs is generated, and a feature vector, or sequence embedding, is developed for each design using natural language processing techniques. The feature vector may be based upon sequence attributes and editing kinetics of each cassette design as well as attributes that describe the library context. Features may include sequence embeddings generated from a neural network, linguistic-type distances, and statistical distance summaries thereof. The feature vectors are classified using one or more machine learning models, and the classified feature vectors are used to predict the representation of each design an edited cell population.

公开(公告)号：US11279919B2

公开(公告)日：2022-03-22

申请号：US17475392

申请日：2021-09-15

Applicant: Inscripta, Inc.

Inventor： Skylar Stefani ,  Tian Tian ,  Miles Gander

IPC: C12N9/22 ,  C12N15/85 ,  C12Q1/44

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20220002694A1

公开(公告)日：2022-01-06

申请号：US17475392

申请日：2021-09-15

Applicant: Inscripta, Inc.

Inventor： Skylar Stefani ,  Tian Tian ,  Miles Gander

IPC: C12N9/22 ,  C12N15/85 ,  C12Q1/44

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US11149260B2

公开(公告)日：2021-10-19

申请号：US17319022

申请日：2021-05-12

Applicant: Inscripta, Inc.

Inventor： Skylar Stefani ,  Tian Tian ,  Miles Gander

IPC: C12N9/22 ,  C12N15/85 ,  C12Q1/44

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US11034945B2

公开(公告)日：2021-06-15

申请号：US17039436

申请日：2020-09-30

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani

IPC: C12N9/22 ,  C12N15/85 ,  C12Q1/44

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US11001831B2

公开(公告)日：2021-05-11

申请号：US17061143

申请日：2020-10-01

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani

IPC: C12N15/10

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20210010006A1

公开(公告)日：2021-01-14

申请号：US16917905

申请日：2020-07-01

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Eileen Spindler

IPC: C12N15/62 ,  C12N15/81

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited yeast cells in a cell population when employing nucleic acid-guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US10752874B2

公开(公告)日：2020-08-25

申请号：US16686066

申请日：2019-11-15

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Miles Gander ,  Don Masquelier ,  Phillip Belgrader ,  Andrew Garst ,  Matthew Estes ,  Bruce Chabansky ,  Jorge Bernate

IPC: C12M1/00 ,  C12M1/26 ,  C12M3/00 ,  C12N15/87 ,  B01D25/00 ,  B01D63/08 ,  B01D29/05 ,  B01D71/02 ,  B01D69/06

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200071657A1

公开(公告)日：2020-03-05

申请号：US16686066

申请日：2019-11-15

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Miles Gander ,  Don Masquelier ,  Phillip Belgrader ,  Andrew Garst ,  Matthew Estes ,  Bruce Chabansky

IPC: C12M1/00 ,  C12N15/87 ,  C12M3/00 ,  C12M1/26

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.