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    Device for detecting microorganisms
    21.
    发明授权
    Device for detecting microorganisms 失效
    微生物检测装置

    公开(公告)号：US5858769A

    公开(公告)日：1999-01-12

    申请号：US648602

    申请日：1996-05-15

    申请人： James L. DiGuiseppi ,  Thurman C. Thorpe

    发明人： James L. DiGuiseppi ,  Thurman C. Thorpe

    IPC分类号： C12M1/24 ,  C12M1/34 ,  G01N21/25 ,  G01N35/00 ,  G01N35/04

    CPC分类号： C12M23/08 ,  C12M23/38 ,  C12M29/20 ,  C12M41/26 ,  C12M41/36 ,  C12Q1/04 ,  G01N21/253 ,  G01N2035/00524

    摘要： An apparatus and method for mixing/agitating microorganism culture bottles includes a wheel in which culture bottles are placed, with the wheel being set at an angle from vertical so that bottles are not inverted during rotation. A sensor such as a florescence or colorimetric sensor for carbon dioxide is disposed along the flat bottom surface of each of the bottles. Once during each revolution the bottles pass across a photodiode/light emitting diode pair (for a calorimetric system). A sensor transmits light emitted by the LED to the photodiode. At the same instant, an infrared reflective objective sensor, emitting on a digital rotary encoder, determines the bottle number. The change in sensor transmittance is directly related to cell growth so that bottles positive for growth can be detected by suitable software.

    摘要翻译： 用于混合/搅拌微生物培养瓶的装置和方法包括其中放置培养瓶的轮，其中轮被设置成垂直于一定角度，使得瓶在旋转期间不反转。 诸如用于二氧化碳的荧光或比色传感器的传感器沿着每个瓶的平坦底表面设置。 每次旋转过程中，瓶子通过光电二极管/发光二极管对（用于量热系统）。 传感器将由LED发射的光传输到光电二极管。 在同一时刻，在数字旋转编码器上发射的红外反射物镜传感器确定瓶子编号。 传感器透射率的变化与细胞生长直接相关，因此可以通过合适的软件检测生长阳性的瓶子。

    Device for detecting microorganisms
    22.
    发明授权
    Device for detecting microorganisms 失效
    微生物检测装置

    公开(公告)号：US5795773A

    公开(公告)日：1998-08-18

    申请号：US823279

    申请日：1997-03-24

    申请人： Karen A. Read ,  David E. Trogdon ,  Thurman C. Thorpe ,  Christopher S. Ronsick ,  Scott R. Jeffrey

    发明人： Karen A. Read ,  David E. Trogdon ,  Thurman C. Thorpe ,  Christopher S. Ronsick ,  Scott R. Jeffrey

    IPC分类号： C12M1/24 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/22 ,  G01N21/25

    CPC分类号： G01N21/253 ,  C12M23/08 ,  C12M23/38 ,  C12M29/20 ,  C12M41/26 ,  C12M41/36 ,  C12Q1/04

    摘要： A method and a sealable, sterilizable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium and a sensor with an indicator medium therein. A gas permeable membrane with a gas impermeable removable seal thereon are provided in a wall of the vessel. If the microorganism to be detected is an anaerobic organism, then the gas impermeable seal is left in place. If the microorganism to be detected is an aerobic organism, then the gas impermeable seal is removed so as to allow the passage of oxygen into the vessel. In a preferred embodiment, the gas permeable membrane allows the passage of oxygen into the vessel while at least partly restricting the passage of carbon dioxide to outside of the vessel. Changes in the indicator medium resulting from a change in gas production (CO.sub.2, NH.sub.2, H.sub.2 S, etc.), volatile acid production or pH concentration in the medium, are detected from outside the vessel.

    摘要翻译： 一种用于检测样品中微生物存在的方法和可密封的可灭菌容器，所述容器含有液体培养基和其中具有指示剂介质的传感器。 在容器的壁上设置有其上具有气体不可渗透的可移除密封的透气膜。 如果要检测的微生物是厌氧生物，则将不透气密封留在原位。 如果要检测的微生物是有氧生物体，则除去气体不可渗透的密封，以允许氧气进入容器。 在优选的实施方案中，透气膜允许氧气进入容器，同时至少部分地限制二氧化碳通过容器的外部。 从容器外部检测到由气体产生（CO2，NH2，H2S等）变化引起的指示剂介质的变化，介质中的挥发性酸产生或pH浓度的变化。