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公开(公告)号：US5795773A
公开(公告)日：1998-08-18
申请号：US823279
申请日：1997-03-24
申请人： Karen A. Read , David E. Trogdon , Thurman C. Thorpe , Christopher S. Ronsick , Scott R. Jeffrey
发明人： Karen A. Read , David E. Trogdon , Thurman C. Thorpe , Christopher S. Ronsick , Scott R. Jeffrey
摘要： A method and a sealable, sterilizable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium and a sensor with an indicator medium therein. A gas permeable membrane with a gas impermeable removable seal thereon are provided in a wall of the vessel. If the microorganism to be detected is an anaerobic organism, then the gas impermeable seal is left in place. If the microorganism to be detected is an aerobic organism, then the gas impermeable seal is removed so as to allow the passage of oxygen into the vessel. In a preferred embodiment, the gas permeable membrane allows the passage of oxygen into the vessel while at least partly restricting the passage of carbon dioxide to outside of the vessel. Changes in the indicator medium resulting from a change in gas production (CO.sub.2, NH.sub.2, H.sub.2 S, etc.), volatile acid production or pH concentration in the medium, are detected from outside the vessel.
摘要翻译： 一种用于检测样品中微生物存在的方法和可密封的可灭菌容器，所述容器含有液体培养基和其中具有指示剂介质的传感器。 在容器的壁上设置有其上具有气体不可渗透的可移除密封的透气膜。 如果要检测的微生物是厌氧生物，则将不透气密封留在原位。 如果要检测的微生物是有氧生物体，则除去气体不可渗透的密封，以允许氧气进入容器。 在优选的实施方案中，透气膜允许氧气进入容器，同时至少部分地限制二氧化碳通过容器的外部。 从容器外部检测到由气体产生（CO2，NH2，H2S等）变化引起的指示剂介质的变化，介质中的挥发性酸产生或pH浓度的变化。
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公开(公告)号：US07183073B2
公开(公告)日：2007-02-27
申请号：US10428655
申请日：2003-05-02
摘要： A device and method are provided for isolating and culturing microorganisms from a bulk fluid sample. The device comprises a container having therein a polymeric immobilization layer having interstitial spaces between polymer chains such as a gel matrix. The interstitial spaces are of an average size less than an average size of microorganisms to be separated from the sample and cultured. A bulk fluid sample is applied to the immobilization layer where fluid is absorbed by the layer and microorganisms remain on the surface of the layer. After culturing, microorganism colonies are readily accessible on the surface of the layer for harvest and testing. The immobilization layer may contain one or more of nutrients for microorganisms growth, lytic agents, lytic enzymes, antibiotics, antibiotic neutralizers, indicators, detergents and selective agents. An adjacent support layer may be above and/or below the immobilization layer. The immobilization layer may be in combination with a sensor layer that changes color in areas corresponding to portions of the layer having microorganisms thereon. A membrane may be embedded in the immobilization layer for enhancing microorganism visibility and facilitating microorganism harvest.
摘要翻译： 提供了用于从大量流体样品中分离和培养微生物的装置和方法。 该装置包括其中具有聚合物固定化层的容器，其在聚合物链之间具有间隙，例如凝胶基质。 间隙空间的平均尺寸小于从样品中分离的微生物的平均尺寸并进行培养。 将大量流体样品施加到固定层，其中流体被层吸收，微生物保留在层的表面上。 培养后，微生物菌落易于在层的表面进行收获和测试。 固定层可以含有一种或多种用于微生物生长的营养物，溶解剂，溶解酶，抗生素，抗生素中和剂，指示剂，洗涤剂和选择剂。 相邻的支撑层可以在固定层的上方和/或下方。 固定层可以与在其上具有微生物层的部分相对应的区域中改变颜色的传感器层组合。 膜可以嵌入在固定层中以增强微生物的可见性并促进微生物收获。
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3.发明授权Device for isolation and surface culture of microorganisms from bulk fluids 有权用于从散装流体中分离和表面培养微生物的装置公开(公告)号：US06596532B1
公开(公告)日：2003-07-22
申请号：US09502324
申请日：2000-02-11
IPC分类号： C12M134
摘要： A device and method are provided for isolating and culturing microorganisms from a bulk fluid sample. The device comprises a container having therein a polymeric immobilization layer having interstitial spaces between polymer chains such as a gel matrix. The interstitial spaces are of an average size less than an average size of microorganisms to be separated from the sample and cultured. A bulk fluid sample is applied to the immobilization layer where fluid is absorbed by the layer and microorganisms remain on the surface of the layer. After culturing, microorganism colonies are readily accessible on the surface of the layer for harvest and testing. The immobilization layer may contain one or more of nutrients for microorganism growth, lytic agents, lytic enzymes, antibiotics, antibiotic neutralizers, indicators, detergents and selective agents. An adjacent support layer may be above and/or below the immobilization layer. The immobilization layer may be in combination with a sensor layer that changes color in areas corresponding to portions of the layer having microorganisms thereon. A membrane may be embedded in the immobilization layer for enhancing microorganism visibility and facilitating microorganism harvest.
摘要翻译： 提供了用于从大量流体样品中分离和培养微生物的装置和方法。 该装置包括其中具有聚合物固定化层的容器，其在聚合物链之间具有间隙，例如凝胶基质。 间隙空间的平均尺寸小于从样品中分离的微生物的平均尺寸并进行培养。 将大量流体样品施加到固定层，其中流体被层吸收，微生物保留在层的表面上。 培养后，微生物菌落易于在层的表面进行收获和测试。 固定层可以含有一种或多种用于微生物生长的营养物，溶解剂，溶解酶，抗生素，抗生素中和剂，指示剂，洗涤剂和选择剂。 相邻的支撑层可以在固定层的上方和/或下方。 固定层可以与在其上具有微生物层的部分相对应的区域中改变颜色的传感器层组合。 膜可以嵌入在固定层中以增强微生物的可见性并促进微生物的收获。
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公开(公告)号：US06416969B2
公开(公告)日：2002-07-09
申请号：US09843202
申请日：2001-04-26
申请人： Paul M. Matsumura , Jones M. Hyman , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe , William G. Barron
发明人： Paul M. Matsumura , Jones M. Hyman , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe , William G. Barron
IPC分类号： C12Q118
CPC分类号： C12M41/46 , C12M41/36 , C12Q1/04 , C12Q1/18 , G01N21/253 , G01N2035/0425
摘要： Disposable plates for performing microbial antibiotic susceptibility testing with multiple channels can be inoculated with a microorganism and antimicrobial agent. The antimicrobial agent may be presented as a gradient of concentrations in the plate. The susceptibility testing plates are configured to allow viewing of microbial growth, and/or to be received in an automated instrument which analyzes same.
摘要翻译： 用多个通道进行微生物抗生素敏感性试验的一次性板可以用微生物和抗微生物剂接种。 抗微生物剂可以以板中的浓度梯度表示。 敏感性测试板被配置为允许观察微生物生长，和/或被接收在分析其的自动化仪器中。
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公开(公告)号：US06197577B1
公开(公告)日：2001-03-06
申请号：US09320386
申请日：1999-05-27
IPC分类号： C12M134
摘要： A device and method allow for detecting the presence of microorganisms in clinical and non-clinical specimens. The device, a sensor, provides an environment to culture microbial organism colonies from a fluid sample, and a means to facilitate microbial detection and quantification, either manually or with an instrument. The sensor has a microorganism immobilization matrix layer and a sensor layer. Detected microbial colonies are immediately available for further testing. The sensor provides an area for accepting a fluid sample, a mechanism to immobilize the fluid sample on an interior surface of the plate, nutrients to facilitate growth of microorganisms in the sample, and a sensor for allowing the detection and/or enumeration of microorganism colonies within the sample.
摘要翻译： 一种装置和方法允许检测临床和非临床标本中微生物的存在。 该装置，传感器，提供了从流体样品培养微生物菌落的环境，以及手动或用仪器促进微生物检测和定量的手段。 传感器具有微生物固定基质层和传感器层。 检测到的微生物菌落立即可用于进一步测试。 传感器提供用于接受流体样品的区域，将流体样品固定在板的内表面上的机制，营养物质以促进样品中微生物的生长，以及用于允许微生物菌落的检测和/或计数的传感器 在样品内。
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公开(公告)号：US6153400A
公开(公告)日：2000-11-28
申请号：US267863
申请日：1999-03-12
申请人： Paul M. Matsumura , Jones M. Hyman , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe , William G. Barron
发明人： Paul M. Matsumura , Jones M. Hyman , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe , William G. Barron
CPC分类号： G01N21/253 , C12Q1/18
摘要： A method and apparatus for performing microbial antibiotic susceptibility testing include disposable, multi-chambered susceptibility plates and an automated plate handler and image acquisition and processing instrument. The susceptibility plates are inoculated with a microorganism (any suitable organism such as bacteria, fungi, protozoa, algae or viruses) and anti-microbial agent(s) are applied such that the microorganism is exposed to a variety of concentrations, or a gradient of each anti-microbial agent. The plates are then placed in the instrument, which monitors and measures the growth (or lack thereof) of the microorganisms. This data is used to determine the susceptibility of the microorganism to the antibiotics. Such a system automates antimicrobial susceptibility testing using solid media and Kirby-Bauer standardized result reporting. The system provides a level of automation previously associated only with broth microdilution testing, while retaining the advantages of the manual disk diffusion test.
摘要翻译： 用于进行微生物抗生素敏感性试验的方法和装置包括一次性多室敏感性板和自动板处理器和图像采集和处理仪器。 用微生物（诸如细菌，真菌，原生动物，藻类或病毒的任何合适的生物体）接种敏感性板，并施用抗微生物剂，使得微生物暴露于多种浓度或梯度 每种抗微生物剂。 然后将板放置在仪器中，其监测和测量微生物的生长（或不存在）。 该数据用于确定微生物对抗生素的敏感性。 这种系统使用固体培养基和Kirby-Bauer标准化结果报告自动化抗微生物药敏试验。 该系统提供了以前仅与肉汤微量稀释测试相关联的自动化水平，同时保留了手动盘扩散测试的优点。
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公开(公告)号：US5976827A
公开(公告)日：1999-11-02
申请号：US989560
申请日：1997-12-12
摘要： A device and method allow for detecting the presence of microorganisms in clinical and non-clinical specimens. The device, a sensor, provides an environment to culture microbial organism colonies from a fluid sample, and a means to facilitate microbial detection and quantification, either manually or with an instrument. The sensor has a microorganism immobilization matrix layer and a sensor layer. Detected microbial colonies are immediately available for further testing. The sensor provides an area for accepting a fluid sample, a mechanism to immobilize the fluid sample on an interior surface of the plate, nutrients to facilitate growth of microorganisms in the sample, and a sensor for allowing the detection and/or enumeration of microorganism colonies within the sample.
摘要翻译： 一种装置和方法允许检测临床和非临床标本中微生物的存在。 该装置，传感器，提供了从流体样品培养微生物菌落的环境，以及手动或用仪器促进微生物检测和定量的手段。 传感器具有微生物固定基质层和传感器层。 检测到的微生物菌落立即可用于进一步测试。 传感器提供用于接受流体样品的区域，将流体样品固定在板的内表面上的机制，营养物质以促进样品中微生物的生长，以及用于允许微生物菌落的检测和/或计数的传感器 在样品内。
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公开(公告)号：US06777226B2
公开(公告)日：2004-08-17
申请号：US09741402
申请日：2000-12-18
IPC分类号： C12M134
摘要： A device and method allow for detecting the presence of microorganisms in clinical and non-clinical specimens. The device, a sensor, provides an environment to culture microbial organism colonies from a fluid sample, and a means to facilitate microbial detection and quantification, either manually or with an instrument. The sensor has a microorganism immobilization matrix layer and a sensor layer. Detected microbial colonies are immediately available for further testing. The sensor provides an area for accepting a fluid sample, a mechanism to immobilize the fluid sample on an interior surface of the plate, nutrients to facilitate growth of microorganisms in the sample, and a sensor for allowing the detection and/or enumeration of microorganism colonies within the sample.
摘要翻译： 一种装置和方法允许检测临床和非临床标本中微生物的存在。 该装置，传感器，提供了从流体样品培养微生物菌落的环境，以及手动或用仪器促进微生物检测和定量的手段。 传感器具有微生物固定基质层和传感器层。 检测到的微生物菌落立即可用于进一步测试。 传感器提供用于接受流体样品的区域，将流体样品固定在板的内表面上的机制，营养物质以促进样品中微生物的生长，以及用于允许微生物菌落的检测和/或计数的传感器 在样品内。
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9.发明授权Apparatus and method for detecting, quantifying and characterizing microorganisms 有权用于检测，定量和表征微生物的装置和方法公开(公告)号：US06251624B1
公开(公告)日：2001-06-26
申请号：US09502678
申请日：2000-02-11
申请人： Paul M. Matsumura , Jones M. Hyman , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe , William G. Barron
发明人： Paul M. Matsumura , Jones M. Hyman , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe , William G. Barron
IPC分类号： C12Q104
CPC分类号： C12M41/46 , C12M41/36 , C12Q1/04 , C12Q1/18 , G01N21/253 , G01N2035/0425
摘要： A method and apparatus for performing microbial antibiotic susceptibility testing include disposable, multi-chambered susceptibility plates and an automated plate handler and image acquisition and processing instrument is described. The susceptibility plates are inoculated with a suitable microorganism such as bacteria, fungi, protozoa, algae or viruses and anti-microbial agent(s) are applied that the microorganism is exposed to at a variety of concentrations or a gradient of each anti-microbial agent. The plates are then placed in the instrument, which monitors and measures the growth or lack thereof of the microorganisms. This data is used to determine the susceptibility of the microorganism to the antibiotics. Such a system automates anti-microbial susceptibility testing using solid media and Carbo-Bauer standardized result reporting. The system provides a level of automation previously associated only with broth micro dilution testing, while retaining the advantages of the manual disk diffusion test.
摘要翻译： 用于进行微生物抗生素敏感性试验的方法和装置包括一次性多室敏感性板和自动板处理器以及图像采集和处理仪器。 敏感性板用合适的微生物如细菌，真菌，原生动物，藻类或病毒接种，并且应用抗微生物剂，微生物以各种浓度或每种抗微生物剂的梯度暴露 。 然后将板放置在仪器中，其监测和测量微生物的生长或缺乏。 该数据用于确定微生物对抗生素的敏感性。 这种系统使用固体培养基和Carbo-Bauer标准化结果报告自动化抗微生物敏感性测试。 该系统提供以前仅与肉汤微量稀释测试相关的自动化水平，同时保留手动盘扩散测试的优点。
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公开(公告)号：US08241867B2
公开(公告)日：2012-08-14
申请号：US12862296
申请日：2010-08-24
摘要： An integrated filtration and detection device for collecting and detecting the growth of microorganisms in a specimen includes a container defining a chamber therein. The container has an inlet and an outlet in fluid communication with the chamber. A filter is mounted in the chamber between the inlet and the outlet. A sensor is mounted in the chamber. The sensor is operative to exhibit a change in a measurable property thereof upon exposure to changes in the chamber due to microbial growth.
摘要翻译： 用于收集和检测试样中微生物生长的集成过滤和检测装置包括在其中限定室的容器。 容器具有与腔室流体连通的入口和出口。 过滤器安装在入口和出口之间的腔室中。 传感器安装在腔室中。 当由于微生物生长而暴露于室内的变化时，该传感器可操作地表现出其可测量性质的变化。
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