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公开(公告)号：US5094955A
公开(公告)日：1992-03-10
申请号：US480398
申请日：1990-02-15
申请人： Michael J. Calandra , James E. Turner , Thurman C. Thorpe , James L. DiGuiseppi , Richard C. Driscoll , Carmella S. Moody
发明人： Michael J. Calandra , James E. Turner , Thurman C. Thorpe , James L. DiGuiseppi , Richard C. Driscoll , Carmella S. Moody
IPC分类号： C12M1/34 , C12Q1/04 , C12Q1/18 , G01N27/416
CPC分类号： G01N21/80 , C12M23/22 , C12M41/26 , C12M41/34 , C12M41/46 , G01N21/6428 , G01N21/78 , Y10S435/807 , Y10T436/235
摘要： An instrument and a sealable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium communicating with a sensor means containing an indicator medium therein. Changes in the indicator medium resulting from pH change or change in CO.sub.2 concentration in the medium are detected from outside the vessel.
摘要翻译： 一种用于检测样品中微生物存在的仪器和可密封容器，所述容器含有与其中含有指示剂介质的传感器装置连通的液体培养基。 从容器外部检测由pH变化引起的指示剂介质的变化或介质中CO 2浓度的变化。
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2.发明授权Device and method for enhanced recovery and detection of microbial growth in the presence of antimicrobial substances 失效在抗菌物质存在下增强微生物生长恢复和检测的装置和方法
公开(公告)号：US5162229A
公开(公告)日：1992-11-10
申请号：US732569
申请日：1991-07-19
申请人： Thurman C. Thorpe , James L. DiGuiseppi , Richard C. Driscoll , James E. Turner , Michael J. Calandra
发明人： Thurman C. Thorpe , James L. DiGuiseppi , Richard C. Driscoll , James E. Turner , Michael J. Calandra
CPC分类号： C12M41/46 , C12M23/22 , C12N1/38 , C12Q1/04 , Y10S435/807 , Y10T436/235
摘要： The present invention relates to a device and method of detecting microorganisms in a rapid manner and increasing the number of microorganisms detected during the culturing of a sample by the addition of resinous and non-resin adsorbents and molecular sieves in the growth media. These agents have been found to neutralize or remove inhibitory and antimicrobial substances found in samples and media.
摘要翻译： 本发明涉及通过在生长培养基中添加树脂和非树脂吸附剂和分子筛，快速检测微生物并增加在培养样品期间检测到的微生物数量的装置和方法。 已经发现这些试剂中和或去除样品和培养基中发现的抑制性和抗微生物物质。
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公开(公告)号：US5217876A
公开(公告)日：1993-06-08
申请号：US866130
申请日：1992-04-07
申请人： James E. Turner , Thurman C. Thorpe , James L. DiGuiseppi , Richard C. Driscoll , Michael J. Calandra
发明人： James E. Turner , Thurman C. Thorpe , James L. DiGuiseppi , Richard C. Driscoll , Michael J. Calandra
IPC分类号： C12M1/34
摘要： An instrument and a sealable, sterilizable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium and a sensor means with an indicator medium therein. Changes in the indicator medium resulting from pH change or change in CO.sub.2 concentration in the medium are detected from outside the vessel.
摘要翻译： 一种用于检测样品中微生物存在的仪器和可密封的可灭菌容器，所述容器含有液体培养基和其中具有指示剂介质的传感器装置。 从容器外部检测由pH变化引起的指示剂介质的变化或介质中CO 2浓度的变化。
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公开(公告)号：US4945060A
公开(公告)日：1990-07-31
申请号：US168291
申请日：1988-03-15
摘要： An instrument and a sealable, sterilizable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium and a sensor means with an indicator medium therein. Changes in the indicator medium resulting from pH change or change in CO.sub.2 concentration in the medium are detected from outside the vessel.
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公开(公告)号：US5068181A
公开(公告)日：1991-11-26
申请号：US443953
申请日：1989-12-01
申请人： Richard C. Driscoll
发明人： Richard C. Driscoll
CPC分类号： G01N33/86 , G01N21/78 , G01N21/314 , Y10S436/80 , Y10T436/13
摘要： A method for measuring the concentration of a reagent in a reaction mixture including the steps of: adding dye to a reagent until the dye is at a given concentration in the reagent; mixing the reagent with a reaction mixture comprising a sample which reacts with the reagent to form a reaction product; measuring the formation of reaction product at a first spectral region; measuring the concentration of dye in the reaction mixture at a second spectral region in which the dye has an optical characteristic such as absorption or fluorescence, the second spectral region being different from the first spectral region; and determining the concentration of the reagent in the reaction mixture based on the concentration of dye measured.In a further aspect of the invention there is provided a reagent containing a dye useful in the above method.
摘要翻译： 一种用于测量反应混合物中试剂浓度的方法，包括以下步骤：向试剂中加入染料，直到染料在试剂中达到给定浓度; 将试剂与包含与试剂反应形成反应产物的样品的反应混合物混合; 测量在第一光谱区域的反应产物的形成; 在染料具有诸如吸收或荧光的光学特性的第二光谱区域测量反应混合物中染料的浓度，第二光谱区域与第一光谱区域不同; 并根据所测染料浓度确定反应混合物中试剂的浓度。 在本发明的另一方面，提供了含有上述方法中有用的染料的试剂。
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6.发明授权
公开(公告)号：US4167449A
公开(公告)日：1979-09-11
申请号：US822057
申请日：1977-08-05
CPC分类号： C12Q1/37 , C07K5/06026 , C12Q1/48 , C12Q2337/10
摘要： Transferase and protease activity in homogenates and biological solutions is fluorometrically determined at wavelengths corresponding generally to those used for fluorometric NADH-linked determinations by utilizing novel substrate compositions consisting essentially of certain fluorogenic 5-aminoisophthalic acid derivatives coupled to amino acid constituents specific to the transferases and proteases under investigation.
摘要翻译： 在匀浆和生物溶液中的转移酶和蛋白酶活性通过利用新型底物组合物来进行荧光测定，所述波长通常对应于用于荧光测定NADH的测定的波长，所述新型底物组合物基本上由与转移酶特异的氨基酸成分偶联的某些荧光5-氨基间苯二甲酸衍生物组成， 正在调查的蛋白酶。
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公开(公告)号：US4115537A
公开(公告)日：1978-09-19
申请号：US720990
申请日：1976-09-07
CPC分类号： G01N33/78 , Y10S436/81
摘要： Resilient polystyrene anion-exchange resin beads in a size range of 50 to 600 mesh are bound together by microcrystalline cellulose to form a tablet suitable for use in conducting clinical diagnostic tests, and particularly, in making thyroxine determinations utilizing competitive protein-binding techniques.
摘要翻译： 尺寸范围为50至600目的弹性聚苯乙烯阴离子交换树脂珠粒通过微晶纤维素结合在一起形成适合用于进行临床诊断试验的片剂，特别是利用竞争性蛋白质结合技术制备甲状腺素。
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