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公开(公告)号：US5439650A
公开(公告)日：1995-08-08
申请号：US970941
申请日：1992-11-03
申请人： Akira Tsugita , Masaharu Kamo , Toyoaki Uchida , Ikuo Nanno , Yasuhiro Nomoto , Seitaro Takahashi
发明人： Akira Tsugita , Masaharu Kamo , Toyoaki Uchida , Ikuo Nanno , Yasuhiro Nomoto , Seitaro Takahashi
IPC分类号： G01N33/50 , B01J19/00 , C07D277/20 , C07D277/40 , C40B40/10 , C40B60/14 , G01N1/28 , B01S8/20 , G05F7/00
CPC分类号： B01J19/0053 , B01J19/0046 , G01N1/28 , B01J2219/00286 , B01J2219/0059 , B01J2219/00725 , C40B40/10 , C40B60/14 , Y10S436/807
摘要： An reaction vessel utilizes a sample carrier composed of a magnetic core and a surface coating effective to support sample of protein or peptide. The sample carrier is floated magnetically and positioned within a reaction chamber. Edman reagent is applied to the sample to effect amino acid sequence analysis of protein or peptide from amino-terminal. By such construction, reaction efficiency is increased to produce sequentially thiazolinon amino-acid derivatives to thereby increase number of identified remaining amino acids, thereby enabling microanalysis of sample.
摘要翻译： 反应容器使用由磁芯和表面涂层组成的样品载体，其有效地支持蛋白质或肽的样品。 样品载体磁性浮动并定位在反应室内。 将Edman试剂应用于样品，以从氨基末端进行蛋白质或肽的氨基酸序列分析。 通过这种结构，提高反应效率以依次产生噻唑啉氨基酸衍生物，从而增加鉴定的残留氨基酸的数量，由此可以进行样品的微量分析。
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22.发明授权Method for amino acid sequencing of protein or peptide from carboxy terminus thereof 失效从羧基末端对蛋白质或肽进行氨基酸测序的方法
公开(公告)号：US6046053A
公开(公告)日：2000-04-04
申请号：US862625
申请日：1997-05-23
CPC分类号： G01N33/6821
摘要： A process is disclosed for sequencing proteins or peptides from the C-terminal end. The process comprises the steps of reacting the peptide or protein with an alkyl acid anhydride to convert the carboxy-terminal thereof into oxazolone, liberating the C-terminal amino acid by reaction with acid and alcohol or with ester, and identifying the liberated amino acid or amino acid derivative.
摘要翻译： 公开了从C末端测序蛋白质或肽的方法。 该方法包括以下步骤：使肽或蛋白质与烷基酸酐反应，将其羧基末端转化成恶唑酮，通过与酸和醇或酯反应释放C端氨基酸，并鉴定释放的氨基酸或 氨基酸衍生物。
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23.发明授权Method of determining amino acid sequence of protein or peptide from carboxy-terminal 失效从羧基末端测定蛋白质或肽的氨基酸序列的方法
公开(公告)号：US5521097A
公开(公告)日：1996-05-28
申请号：US931931
申请日：1992-08-18
申请人： Toyoaki Uchida , Akira Tsugita , Keiji Takamoto , Kazuo Satake
发明人： Toyoaki Uchida , Akira Tsugita , Keiji Takamoto , Kazuo Satake
CPC分类号： G01N33/6821 , C07K1/128 , Y10T436/24
摘要： A protein or peptide is treated by a vapor containing an organic acid represented by the following general formula CF.sub.3 --(CF.sub.2)n--COOH (n is zero or more integer). The resulting reaction mixture is processed by a mass spectrometer to obtain a mass spectrum to measure a mass of respective chemical species contained in the reaction mixture. Alternatively, the reaction mixture is processed by an amino acid analyzer to determine an amino acid sequence of the protein or peptide from the carboxy-terminal. According to another method, the protein or peptide is treated by an anhydride of the organic acid represented by the following general formula CF.sub.3 --(CF.sub.2)n--COOH (n is zero or more integer). The resulting reaction mixture is processed by a mass spectrometer to obtain a mass spectrum to measure a mass of respective chemical species contained in the reaction mixture to determine an amino acid sequence of the protein or peptide from the carboxy-terminal.
摘要翻译： 通过含有由以下通式CF 3 - （CF 2）n -COOH表示的有机酸的蒸气处理蛋白质或肽（n为0以上整数）。 通过质谱仪处理得到的反应混合物，得到质谱以测量反应混合物中各化学物质的质量。 或者，通过氨基酸分析仪处理反应混合物以从羧基端确定蛋白质或肽的氨基酸序列。 根据另一种方法，蛋白质或肽由下列通式CF 3 - （CF 2）n -COOH表示的有机酸的酸酐（n为0以上整数）处理。 通过质谱仪处理所得反应混合物以获得质谱以测量反应混合物中所含的各化学物质的质量，以确定羧基末端的蛋白质或肽的氨基酸序列。
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公开(公告)号：US4865994A
公开(公告)日：1989-09-12
申请号：US185324
申请日：1988-04-19
申请人： Akira Tsugita , Isamu Arai , Tatsuaki Ataka
发明人： Akira Tsugita , Isamu Arai , Tatsuaki Ataka
IPC分类号： C07B59/00 , C07K1/12 , G01N33/533 , G01N33/534 , G01N33/68 , G01N33/76
CPC分类号： C07K1/12 , G01N33/533 , G01N33/534 , G01N33/6809 , G01N33/6824 , Y10T436/141111
摘要： A method for detecting amino acid derivatives in which 2-anilino-5-thiazolinone derivatives of amino acids are reacted with radioactive iodine siotope-labeled amino compounds or with fluorescent amino compounds to form phenylthiocarbamyl amino acid derivatives. The phenylthiocarbamyl amino acid derivatives are detected with a high level of sensitivity by a radioactive detector or a fluorescence spectrophotometer.
摘要翻译： 用于检测氨基酸的2-苯胺基-5-噻唑啉酮衍生物与放射性碘素标记的氨基化合物或与荧光氨基化合物反应以形成苯基硫代氨基甲酰基氨基酸衍生物的氨基酸衍生物的方法。 通过放射性检测器或荧光分光光度计以高灵敏度检测苯硫基氨基甲酰氨基酸衍生物。
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公开(公告)号：US5962642A
公开(公告)日：1999-10-05
申请号：US854187
申请日：1997-05-09
CPC分类号： G01N33/6842 , C07K7/06 , G01N33/6824
摘要： A protein or peptide which has an amino-terminus serine or threonine which has an acetylated alpha-amino group is allowed to react with an acid, and then allowed to react with an isothiocyanate under acidic conditions to thereby obtain a thiocarbamyl compound. Then the compound is to be analyzed using Edman degradation. Analysis can be performed with fewer operation steps and without using enzymes.
摘要翻译： 具有具有乙酰化α-氨基的氨基末端丝氨酸或苏氨酸的蛋白质或肽与酸反应，然后在酸性条件下与异硫氰酸酯反应，从而得到硫代氨基甲酰基化合物。 然后使用Edman降解分析化合物。 可以用较少的操作步骤进行分析，而不使用酶。
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公开(公告)号：US5051369A
公开(公告)日：1991-09-24
申请号：US227184
申请日：1988-08-02
申请人： Akira Tsugita , Toyoaki Uchida
发明人： Akira Tsugita , Toyoaki Uchida
IPC分类号： C07D311/86 , G01N33/52 , G01N33/68
CPC分类号： G01N33/6824 , G01N33/52 , Y10T436/141111
摘要： A method for detecting amino acid derivatives in which 2-anilino-5-thiazolinone derivatives of amino acids are reacted with an amino compound of fluorescein or rhodamine derivative amino compounds to form phenylthiocarbamyl amino acids derivatives. The phenylithiocarbamyl amino acids derivatives are detected with a high level of sensitivity by a fluorescence spectrophotometer.
摘要翻译： 用于检测其中氨基酸的2-苯胺基-5-噻唑啉酮衍生物与荧光素或罗丹明衍生物氨基化合物的氨基化合物反应以形成苯基硫代氨基甲酰基氨基酸衍生物的氨基酸衍生物的方法。 通过荧光分光光度计以高灵敏度检测苯硫基氨基甲酰氨基酸衍生物。
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