公开(公告)号：US20240210410A1

公开(公告)日：2024-06-27

申请号：US18438351

申请日：2024-02-09

申请人： ABRUS BIO, INC.

发明人： Michael Graige ,  Christopher MacDonald

IPC分类号： G01N33/68 ,  C12Q1/6804 ,  C12Q1/6869 ,  G01N33/58

CPC分类号： G01N33/6824 ,  C12Q1/6804 ,  C12Q1/6869 ,  G01N33/58 ,  G01N2458/10

摘要： The present disclosure relates to compositions of matter, methods, and systems for analyzing polymeric macromolecules, including polymeric macromolecules such as peptides, polypeptides, and proteins.

公开(公告)号：US12019077B2

公开(公告)日：2024-06-25

申请号：US17197796

申请日：2021-03-10

申请人： Encodia, Inc.

发明人： Mark S. Chee ,  Kevin L. Gunderson ,  Michael Phillip Weiner

IPC分类号： G01N33/68 ,  C40B70/00

CPC分类号： G01N33/6824 ,  C40B70/00 ,  G01N33/6803 ,  G01N33/6845 ,  G01N2458/00 ,  G01N2458/10

摘要： A method for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding is disclosed.

公开(公告)号：US20240201199A1

公开(公告)日：2024-06-20

申请号：US18524884

申请日：2023-11-30

申请人： Washington University

发明人： James J. Havranek ,  Benjamin Borgo

IPC分类号： G01N33/68 ,  C12N9/00 ,  C12N9/48 ,  C12N9/52 ,  C12N9/64

CPC分类号： G01N33/6824 ,  C12N9/485 ,  C12N9/52 ,  C12N9/641 ,  C12N9/93 ,  C12Y304/11018 ,  C12Y601/0101 ,  C12Y601/0102 ,  C12Y601/01021

摘要： Reagents and methods for the digital analysis of proteins or peptides are provided. Specifically provided herein are proteins for identifying the N-terminal amino acid or N-terminal phosphorylated amino acid of a polypeptide. Also, an enzyme for use in the cleavage step of the Edman degradation reaction and a method for using this enzyme are described.

公开(公告)号：US12000835B2

公开(公告)日：2024-06-04

申请号：US16709052

申请日：2019-12-10

申请人： Quantum-Si Incorporated

发明人： Brian Reed ,  Jeremy Lackey ,  Haidong Huang

IPC分类号： G01N33/68 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/64 ,  G01N33/58 ,  G16B25/10 ,  G16B30/00 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G16B50/00

CPC分类号： G01N33/581 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/6428 ,  G01N33/58 ,  G01N33/582 ,  G01N33/6821 ,  G01N33/6824 ,  G16B25/10 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G01N2021/6439 ,  G01N2458/00 ,  G16B30/00 ,  G16B50/00

摘要： Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules having a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US11959920B2

公开(公告)日：2024-04-16

申请号：US16708989

申请日：2019-12-10

申请人： Quantum-Si Incorporated

发明人： Brian Reed ,  Jeremy Lackey ,  Haidong Huang

IPC分类号： G01N33/58 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/64 ,  G01N33/68 ,  G16B25/10 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G16B30/00 ,  G16B50/00

CPC分类号： G01N33/581 ,  C07K14/47 ,  C07K19/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N21/6428 ,  G01N33/58 ,  G01N33/582 ,  G01N33/6821 ,  G01N33/6824 ,  G16B25/10 ,  G16B40/00 ,  G16B40/10 ,  G16B50/30 ,  G01N2021/6439 ,  G01N2458/00 ,  G16B30/00 ,  G16B50/00

摘要： Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

公开(公告)号：US11906525B2

公开(公告)日：2024-02-20

申请号：US17824073

申请日：2022-05-25

申请人： Massachusetts Institute of Technology

发明人： Edward Boyden ,  Adam Henry Marblestone ,  Samuel Gordon Rodriques

IPC分类号： G01N33/68 ,  C12Q1/37 ,  G01N21/64 ,  G01N21/76

CPC分类号： G01N33/6824 ,  C12Q1/37 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/76 ,  G01N2333/948

摘要： The invention, in part, includes methods of single molecule protein sequencing that include using weak binding spectra in the amino acid identification.

公开(公告)号：US09566335B1

公开(公告)日：2017-02-14

申请号：US12891313

申请日：2010-09-27

申请人： Andrew Emili ,  Megan McLaughlin ,  Kyrylo Zagorovsky ,  Jonathan Buchanan Olsen ,  Warren C. W. Chan ,  Sachdev S. Sidhu

发明人： Andrew Emili ,  Megan McLaughlin ,  Kyrylo Zagorovsky ,  Jonathan Buchanan Olsen ,  Warren C. W. Chan ,  Sachdev S. Sidhu

IPC分类号： G01N31/00 ,  G01N33/53 ,  A61K45/06

CPC分类号： G01N33/6824 ,  A61K45/06 ,  C07K14/245 ,  G01N33/582 ,  G01N2333/952

摘要： The invention describes methods and reagents useful for sequencing polypeptide molecules. The method comprises affixing a polypeptide to a substrate and contacting the polypeptide with a plurality of probes. Each probe selectively binds to an N-terminal amino acid or an N-terminal amino acid derivative. Probes bound to the polypeptide molecule are then identified before cleaving the N-terminal amino acid or N-terminal amino acid derivative of the polypeptide. Also provided are methods for the sequencing a plurality of polypeptide molecules in a sample and probes specific for N-terminal amino acids or N-terminal amino acid derivatives.

摘要翻译： 本发明描述了可用于测序多肽分子的方法和试剂。 该方法包括将多肽粘附到底物上并使多肽与多个探针接触。 每个探针选择性地结合N-末端氨基酸或N-末端氨基酸衍生物。 然后在切割多肽的N末端氨基酸或N-末端氨基酸衍生物之前鉴定与多肽分子结合的探针。 还提供了用于测序样品中的多个多肽分子和对N-末端氨基酸或N-末端氨基酸衍生物具有特异性的探针的方法。

公开(公告)号：US20140349860A1

公开(公告)日：2014-11-27

申请号：US14128247

申请日：2012-06-22

申请人： Edward Marcotte ,  Jagannath Swaminathan ,  Andrew Wllington ,  Eric Anslyn

发明人： Edward Marcotte ,  Jagannath Swaminathan ,  Andrew Wllington ,  Eric Anslyn

IPC分类号： G01N33/68

CPC分类号： G01N33/6824 ,  G01N2570/00

摘要： The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.

摘要翻译： 本发明涉及鉴定肽中氨基酸的方法。 在一个实施方案中，本发明考虑用第一标记物标记N-末端氨基酸并用第二标记物标记内部氨基酸。 在一些实施方案中，标记是荧光标记。 在其它实施方案中，内部氨基酸是赖氨酸。 在其它实施方案中，肽中的氨基酸基于每个肽在单分子水平的荧光特征来鉴定。

公开(公告)号：US20140273004A1

公开(公告)日：2014-09-18

申请号：US14211448

申请日：2014-03-14

申请人： WASHINGTON UNIVERSITY

发明人： James J. Havranek ,  Benjamin Borgo

IPC分类号： G01N33/68 ,  C12N9/50

CPC分类号： G01N33/6824 ,  C12N9/485 ,  C12N9/50 ,  C12N9/641 ,  C12N9/93 ,  C12Y304/11018 ,  C12Y601/0101 ,  C12Y601/0102 ,  C12Y601/01021

摘要： Reagents and methods for the digital analysis of proteins or peptides are provided. Specifically provided herein are proteins for identifying the N-terminal amino acid or N-terminal phosphorylated amino acid of a polypeptide. Also, an enzyme for use in the cleavage step of the Edman degradation reaction and a method for using this enzyme are described.

摘要翻译： 提供了蛋白质或肽数字分析的试剂和方法。 本文具体提供的是用于鉴定多肽的N-末端氨基酸或N-末端磷酸化氨基酸的蛋白质。 另外，描述了用于Edman降解反应的切割步骤的酶和使用该酶的方法。

公开(公告)号：US20110212531A1

公开(公告)日：2011-09-01

申请号：US13093576

申请日：2011-04-25

申请人： Mario Cindric ,  Sandra Kraljevic Pavelic ,  Anita Horvatic ,  Ivana Dodig

发明人： Mario Cindric ,  Sandra Kraljevic Pavelic ,  Anita Horvatic ,  Ivana Dodig

IPC分类号： G01N27/00

CPC分类号： G01N33/6848 ,  C07K1/107 ,  G01N33/6824 ,  G01N33/6851 ,  G01N2458/15 ,  Y10T436/24 ,  Y10T436/25

摘要： A method of detection of amino acid sequences and/or identification of proteins and peptides is based on derivatization of peptides or proteins using compounds comprising two or more sulfonyl groups, and subsequent analysis of the derivatized analytes using a mass spectrometer in its negative mode of operation.

摘要翻译： 检测氨基酸序列和/或鉴定蛋白质和肽的方法是基于使用包含两个或更多个磺酰基的化合物对肽或蛋白质的衍生化，并且使用在其负操作模式中的质谱仪随后分析衍生的分析物 。