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    Antifungal proteins and methods of use
    21.
    发明授权
    Antifungal proteins and methods of use 有权
    抗真菌蛋白及其使用方法

    公开(公告)号：US08853490B2

    公开(公告)日：2014-10-07

    申请号：US13240410

    申请日：2011-09-22

    申请人： James J. English ,  Azalea S. Ong ,  Nasser Yalpani

    发明人： James J. English ,  Azalea S. Ong ,  Nasser Yalpani

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C07H21/02 ,  C12N15/00 ,  C12N15/82 ,  A01N63/00 ,  C07K14/00 ,  A01N37/46 ,  A01N63/04 ,  A01N63/02

    CPC分类号： C12N15/8209 ,  A01N37/46 ,  A01N63/00 ,  A01N63/02 ,  A01N63/04 ,  C07K14/001 ,  C07K14/415 ,  C12N15/8282 ,  A01N65/00

    摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for novel variants of antipathogenic polypeptides generated through DNA shuffling that exhibit improved antipathogenic activity. Polynucleotides that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the polynucleotides disclosed herein is further provided. Compositions comprising an antipathogenic polypeptide or a microorganism comprising an antipathogenic polynucleotide of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Plants, plant cells, seeds, and microorganisms comprising an antipathogenic polynucleotide or polypeptide of the invention are also disclosed.

    摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括氨基酸序列及其变体和片段，其通过显示改善的抗病原活性的DNA改组产生的抗病原多肽的新变体。 也提供了编码抗病原多肽的多核苷酸。 还提供使用本文公开的多核苷酸在植物中诱导病原体抗性的方法。 包含抗病原体多肽或包含本发明的抗病原体多核苷酸与载体组合的组合物以及使用这些组合物保护植物免受病原体的方法。 还公开了包含本发明的抗病原体多核苷酸或多肽的植物，植物细胞，种子和微生物。

    Antifungal polypeptides
    23.
    发明授权
    Antifungal polypeptides 有权
    抗真菌多肽

    公开(公告)号：US07923599B2

    公开(公告)日：2011-04-12

    申请号：US11833535

    申请日：2007-08-03

    申请人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Elleskaya ,  Natalia Ellanskaya, legal representative ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

    发明人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Elleskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N15/87 ,  C07H21/04

    CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

    摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

    摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

    Maize antimicrobial nucleic acids useful for enhancing plant resistance to pathogens
    25.
    发明授权
    Maize antimicrobial nucleic acids useful for enhancing plant resistance to pathogens 失效
    玉米抗微生物核酸可用于增强植物对病原体的抗性

    公开(公告)号：US07714184B2

    公开(公告)日：2010-05-11

    申请号：US12350264

    申请日：2009-01-08

    申请人： Mathias L. Muller ,  Carl R. Simmons ,  Nasser Yalpani

    发明人： Mathias L. Muller ,  Carl R. Simmons ,  Nasser Yalpani

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/29 ,  C12N15/82

    CPC分类号： C12N9/22 ,  C12N15/8282

    摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from maize. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the embodiments are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant a DNA construct comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the embodiments in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.

    摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从玉米分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码实施方案的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码实施方案的抗病原体多肽的核苷酸序列的启动子的DNA构建体。 还提供包含抗病原体多肽或包含实施方案的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码实施方案的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

    ANTIFUNGAL POLYPEPTIDES
    26.
    发明申请
    ANTIFUNGAL POLYPEPTIDES 有权
    抗菌多糖

    公开(公告)号：US20090264351A1

    公开(公告)日：2009-10-22

    申请号：US11833598

    申请日：2007-08-03

    申请人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Ellanskaya ,  Natalia Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

    发明人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Ellanskaya ,  Natalia Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

    IPC分类号： A01N37/18 ,  C07K14/00 ,  A01P3/00

    CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

    摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

    摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

    Antipathogenic Proteins and Methods of Use
    28.
    发明申请
    Antipathogenic Proteins and Methods of Use 有权
    抗病原体蛋白和使用方法

    公开(公告)号：US20120054910A1

    公开(公告)日：2012-03-01

    申请号：US13205731

    申请日：2011-08-09

    申请人： Daniel J. Altier ,  Jacob T. Gilliam ,  Eric J. Schepers ,  Nasser Yalpani

    发明人： Daniel J. Altier ,  Jacob T. Gilliam ,  Eric J. Schepers ,  Nasser Yalpani

    IPC分类号： A01H1/00 ,  C07K14/37 ,  A61K38/16 ,  A01H5/10 ,  C12N1/21 ,  C12N1/19 ,  C12N5/10 ,  A01H5/00 ,  C12N15/31 ,  C12N1/00

    CPC分类号： C07K14/37 ,  A01N63/04 ,  C12N15/8282

    摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for family members of a novel family of antipathogenic polypeptides. Polynucleotides that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the polynucleotides disclosed herein is further provided. Compositions comprising an antipathogenic polypeptide or a microorganism comprising an antipathogenic polynucleotide of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Plants, plant cells, seeds, and microorganisms comprising an antipathogenic polnucleotide or polypeptide of the invention are also disclosed.

    摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括氨基酸序列及其变体和片段，用于新型抗病原体多肽家族的家族成员。 也提供了编码抗病原多肽的多核苷酸。 还提供使用本文公开的多核苷酸在植物中诱导病原体抗性的方法。 包含抗病原体多肽或包含本发明的抗病原体多核苷酸与载体组合的组合物以及使用这些组合物保护植物免受病原体的方法。 还公开了包含本发明的抗病原性核苷酸或多肽的植物，植物细胞，种子和微生物。

    Nucleic acids encoding antifungal polypeptides and uses thereof
    29.
    发明授权
    Nucleic acids encoding antifungal polypeptides and uses thereof 有权
    编码抗真菌多肽的核酸及其用途

    公开(公告)号：US07825294B2

    公开(公告)日：2010-11-02

    申请号：US12503952

    申请日：2009-07-16

    申请人： Daniel J. Altier ,  I. A. Ellanskaya ,  Jacob T. Gilliam ,  Jennie Hunter-Cevera ,  James K Presnail ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

    发明人： Daniel J. Altier ,  I. A. Ellanskaya ,  Jacob T. Gilliam ,  Jennie Hunter-Cevera ,  James K Presnail ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/09 ,  C12N15/82

    CPC分类号： C12N15/8282 ,  A01N37/46 ,  A01N63/04 ,  C07K14/37

    摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

    摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从真菌发酵液中分离的抗病原体多肽的氨基酸序列及其变体和片段。 还提供了编码本发明的抗病原体多肽的核酸分子及其抗病原结构域。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列的转化植物，植物细胞，种子和微生物。

    Antifungal polypeptides
    30.
    发明授权
    Antifungal polypeptides 有权
    抗真菌多肽

    公开(公告)号：US07772370B2

    公开(公告)日：2010-08-10

    申请号：US11833598

    申请日：2007-08-03

    申请人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Elleskaya ,  Natalia Ellanskaya, legal representative ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

    发明人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Elleskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

    IPC分类号： C07K14/00 ,  C07K14/415

    CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

    摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

    摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。