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    METHOD FOR CREATING PERFUSABLE MICROVESSEL SYSTEMS
    21.
    发明申请
    METHOD FOR CREATING PERFUSABLE MICROVESSEL SYSTEMS 有权
    创造可靠的微电子系统的方法

    公开(公告)号：US20090317787A1

    公开(公告)日：2009-12-24

    申请号：US12541707

    申请日：2009-08-14

    申请人： Thomas Neumann

    发明人： Thomas Neumann

    IPC分类号： A01N1/02 ,  C12N5/08

    CPC分类号： C12N5/0691 ,  C12M21/08 ,  C12M23/06 ,  C12M23/16 ,  C12M23/20 ,  C12M29/10 ,  C12N2533/50

    摘要： Creating a tissue structure in vitro includes juxtaposing mandrels on a culture/perfusion device frame where the mandrels are spaced apart substantially parallel to each other and connecting the mandrels to tubes including an upstream tubes and downstream tubes. The upstream tubes are connected with an upstream manifold and the downstream tubes are connected to a downstream manifold. The frame and the mandrels are sterilized, coated and seeded with cells that multiply and form circular layers around each of the mandrels until the circular layers merge into a tissue structure which is subjected to a growth medium. The mandrels are extracted and the tissue structure is perfused.

    摘要翻译： 在体外创建组织结构包括在培养/灌注装置框架上并置心轴，其中心轴彼此间隔开并且将心轴连接到包括上游管和下游管的管。 上游管与上游歧管连接，下游管连接到下游歧管。 框架和心轴被消毒，涂覆并种植有细胞，每个细胞在每个心轴周围形成圆形层，直到圆形层合并成经历生长培养基的组织结构。 提取心轴并灌注组织结构。

    PROCESS FOR THE TREATMENT OF SiC-BONDED POLYETHERSILOXANES
    23.
    发明申请
    PROCESS FOR THE TREATMENT OF SiC-BONDED POLYETHERSILOXANES 审中-公开
    用于处理SiC结合的聚乙二醇的方法

    公开(公告)号：US20080227923A1

    公开(公告)日：2008-09-18

    申请号：US11755441

    申请日：2007-05-30

    申请人： Klaus-Deiter Klein ,  Thomas Neumann

    发明人： Klaus-Deiter Klein ,  Thomas Neumann

    IPC分类号： C08F8/04

    CPC分类号： C08G77/46 ,  C08G77/34

    摘要： The invention relates to a process for the treatment of polyoxyalkylene-polysiloxane block copolymers or alkylpolysiloxane-polyoxyalkylenepolysiloxane block copolymers in which the polysiloxane blocks are bonded to the polyether blocks or alkyl radicals by SiC bonds, wherein the polyoxyalkylene-polysiloxane block copolymers or alkylpolysiloxane-polyoxyalkylenepolysiloxane block copolymers or the solutions thereof are treated with flowing hydrogen gas and optionally a further inert gas in the presence of a combination of hydrogenation catalysts known per se and acid-activated carrier materials and water at temperatures of from 20 to 200° C. and atmospheric pressure over a period of from 0.5 to 10 hours.

    摘要翻译： 本发明涉及一种用于处理聚氧化烯 - 聚硅氧烷嵌段共聚物或烷基聚硅氧烷 - 聚氧亚烷基聚硅氧烷嵌段共聚物的方法，其中聚硅氧烷嵌段通过SiC键与聚醚嵌段或烷基键合，其中聚氧化烯 - 聚硅氧烷嵌段共聚物或烷基聚硅氧烷 - 聚氧亚烷基聚硅氧烷 嵌段共聚物或其溶液在流动的氢气和任选的另外的惰性气体下，在本身已知的氢化催化剂和酸活化的载体材料和水的温度下，在20〜200℃的温度和大气压下进行处理 压力为0.5〜10小时。

    Method for creating perfusable microvessel systems
    24.
    发明申请
    Method for creating perfusable microvessel systems 有权
    创建可灌注微血管系统的方法

    公开(公告)号：US20070224677A1

    公开(公告)日：2007-09-27

    申请号：US11388920

    申请日：2006-03-24

    申请人： Thomas Neumann

    发明人： Thomas Neumann

    IPC分类号： C12N5/08 ,  C12M3/00 ,  C12N5/06

    CPC分类号： C12N5/0691 ,  C12M21/08 ,  C12M23/06 ,  C12M23/16 ,  C12M23/20 ,  C12M29/10 ,  C12N2533/50

    摘要： Microvessel networks are produced in vitro from tissue-engineered parent vessels sprouting into a supporting matrix, as for example gels, of extracellular matrix proteins. The microvessel systems are integrated into devices that allow for controlled perfusion with fluids. The vessels may include cells from one cell type, for example, endothelial cells, or from combinations of two or more cell types.

    摘要翻译： 微血管网络在体外从组织工程化的母体血管发育成支持基质，例如凝胶，细胞外基质蛋白。 微血管系统集成到允许用流体控制灌注的装置中。 血管可以包括来自一种细胞类型的细胞，例如内皮细胞，或两种或多种细胞类型的组合。

    Method for characterizing polypeptides
    25.
    发明授权
    Method for characterizing polypeptides 失效
    表征多肽的方法

    公开(公告)号：US07163803B2

    公开(公告)日：2007-01-16

    申请号：US10479843

    申请日：2002-06-07

    申请人： Christian Hamon ,  Andrew Thompson ,  Thomas Neumann ,  Robert Johnstone ,  Abdul Karim Abed Mohammed

    发明人： Christian Hamon ,  Andrew Thompson ,  Thomas Neumann ,  Robert Johnstone ,  Abdul Karim Abed Mohammed

    IPC分类号： C12Q1/37

    CPC分类号： G01N33/6848 ,  C07K1/12 ,  G01N33/6821

    摘要： Provided is a method for characterizing a polypeptide, which method comprises the steps of: (a) optionally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analyzing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.

    摘要翻译： 提供了表征多肽的方法，该方法包括以下步骤：（a）任选地减少多肽中的半胱氨酸二硫键形成游离硫醇，并封端游离硫醇; （b）用序列特异性切割试剂切割多肽以形成肽片段; （c）任选地使切割试剂失活; （d）封端与赖氨酸反应剂一起存在的一个或多个ε-氨基; （e）通过质谱分析肽片段以形成多肽的质量指纹图谱; 和（f）从质量指纹图谱确定多肽的身份。

    Mass labels
    29.
    发明授权
    Mass labels 有权
    批量标签

    公开(公告)号：US07732378B2

    公开(公告)日：2010-06-08

    申请号：US10489341

    申请日：2002-09-16

    申请人： Andrew Hugin Thompson ,  Christian Hamon ,  Jurgen Schafer ,  Karsten Kuhn ,  Joseph Schwarz ,  Thomas Neumann

    发明人： Andrew Hugin Thompson ,  Christian Hamon ,  Jurgen Schafer ,  Karsten Kuhn ,  Joseph Schwarz ,  Thomas Neumann

    IPC分类号： C40B40/00

    CPC分类号： G01N33/6848 ,  G01N33/68 ,  G01N33/6851 ,  G01N2458/15 ,  H01J49/04

    摘要： Provided is a set of two or more mass labels, each label in the set comprising a mass marker moiety attached via a cleavable linker having at least one amide bond to a mass normalization moiety, wherein the aggregate mass of each label in the set may be the same or different and the mass of the mass marker moiety of each label in the set may be the same or different, and wherein in any group of labels within the set having a mass marker moiety of a common mass each label has an aggregate mass different from all other labels in that group, and wherein in any group of labels within the set having a common aggregate mass each label has a mass marker moiety having a mass different from that of all other mass marker moieties in that group, such that all of the mass labels in the set are distinguishable from each other by mass spectrometry, and wherein the mass marker moiety comprises an amino acid and the mass normalization moiety comprises an amino acid.

    摘要翻译： 提供了一组两个或更多个质量标签，该组中的每个标签包含通过具有至少一个酰胺键与质量标准化部分连接的质量标记部分，其中该组中每个标签的总质量可以是 相同或不同，组中每个标记物的质量标记物部分的质量可以相同或不同，并且其中在具有共同质量的质量标记物部分的组中的任何标签组中，每个标签具有总体质量 不同于该组中的所有其它标记，并且其中在组中具有共同聚集体的任何标签组中，每个标记具有与该组中所有其他质量标记部分的质量不同的质量标记部分，使得全部 组中的质量标记物通过质谱法彼此区分，并且其中质量标记物部分包含氨基酸，质量标准化部分包含氨基酸。

    Method for charcterising analytes
    30.
    发明授权
    Method for charcterising analytes 失效
    分析物识别方法

    公开(公告)号：US07700306B2

    公开(公告)日：2010-04-20

    申请号：US10510246

    申请日：2003-04-04

    申请人： Andrew H. Thompson ,  Christian Hamon ,  Karsten Kuhn ,  Markus Meyer ,  Schafer Juergen ,  Thomas Neumann

    发明人： Andrew H. Thompson ,  Christian Hamon ,  Karsten Kuhn ,  Markus Meyer ,  Schafer Juergen ,  Thomas Neumann

    IPC分类号： G01N33/00

    CPC分类号： G01N33/6848 ,  C07K1/13 ,  G01N33/6851

    摘要： Provided is a method for characterizing an analyte by matrix assisted laser desorption ionization (MALDI) mass spectrometry, which method comprises: (a) labelling the analyte with a light-absorbing label that absorbs light at a pre-determined frequency, to form a labelled analyte; (b) embedding the labelled analyte in a matrix formed from at least one compound that absorbs light, to form an embedded labelled analyte; (c) desorbing the embedded labelled analyte by exposing it to light having the pre-determined frequency, to form a desorbed analyte; and (d) detecting the desorbed analyte by mass spectrometry to characterize the analyte.

    摘要翻译： 本发明提供了一种通过基质辅助激光解吸电离（MALDI）质谱法分析分析物的方法，该方法包括：（a）用预吸收光量的光吸收标记标记分析物，以形成标记的 分析物; （b）将标记的分析物包埋在由吸收光的至少一种化合物形成的基质中，以形成嵌入的标记分析物; （c）通过将嵌入的标记分析物暴露于具有预定频率的光来解吸，形成解吸附的分析物; 和（d）通过质谱检测解吸附的分析物来表征分析物。