公开(公告)号：US10739341B2

公开(公告)日：2020-08-11

申请号：US14378929

申请日：2013-02-14

申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED

发明人： Daniel John Turner ,  Daniel George Fordham ,  Roger Charles Gill ,  Clive Gavin Brown ,  Stuart Reid ,  James Anthony Clarke ,  James White

IPC分类号： G01N33/00 ,  G01N33/543 ,  G01N33/53 ,  C12N15/115 ,  C07H21/04

摘要： The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.

公开(公告)号：US10221450B2

公开(公告)日：2019-03-05

申请号：US14773164

申请日：2014-01-22

申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED

发明人： Andrew John Heron ,  David Antoni Alves ,  James Anthony Clarke ,  Marion Louise Crawford ,  Daniel Ryan Garalde ,  Graham Hall ,  Daniel John Turner ,  James White

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6827 ,  C12N9/14 ,  C12N13/00

摘要： The invention relates to new methods of moving helicases past spacers on polynucleotides and controlling the loading of helicases on polynucleotides. The invention also relates to new methods of characterizing target polynucleotides using helicases.

公开(公告)号：US10077471B2

公开(公告)日：2018-09-18

申请号：US14858138

申请日：2015-09-18

申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED

发明人： Lakmal Jayasinghe ,  John Hagan Pryce Bayley ,  Stephen Cheley ,  Brian McKeown ,  James White ,  James Anthony Clarke

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12N9/12 ,  C12N9/22 ,  C12N9/52 ,  C07K14/31 ,  C12N9/16 ,  C12N9/90 ,  C12N9/96

CPC分类号： C12Q1/6869 ,  C07K14/31 ,  C12N9/1247 ,  C12N9/1252 ,  C12N9/127 ,  C12N9/1276 ,  C12N9/16 ,  C12N9/22 ,  C12N9/52 ,  C12N9/90 ,  C12N9/96 ,  C12Q2565/631

摘要： The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

公开(公告)号：US20180037874A9

公开(公告)日：2018-02-08

申请号：US15028651

申请日：2014-09-10

申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED

发明人： Mark Bruce ,  Andrew John Heron ,  Ruth Moysey ,  Szabolcs Soeroes ,  Elizabeth Jayne Wallace ,  James White

IPC分类号： C12N9/14 ,  C12Q1/68

摘要： The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

公开(公告)号：US20170067101A1

公开(公告)日：2017-03-09

申请号：US15120186

申请日：2015-02-19

申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED

发明人： James Anthony Clarke ,  Marion Louise Crawford ,  James White

IPC分类号： C12Q1/68

摘要： The invention relates to an improved method for characterising a template polynucleotide. The method involves using a polymerase to prepare a modified polynucleotide which makes it easier to characterise than the template polynucleotide.

摘要翻译： 本发明涉及用于表征模板多核苷酸的改进方法。 该方法涉及使用聚合酶来制备修饰的多核苷酸，使其比模板多核苷酸更易于表征。