-
公开(公告)号：US20210180124A1
公开(公告)日：2021-06-17
申请号：US17094571
申请日：2020-11-10
IPC分类号： C12Q1/6869 , G01N27/447 , G01N33/487 , B82Y15/00
摘要： The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
-
公开(公告)号：US09885078B2
公开(公告)日：2018-02-06
申请号：US14455394
申请日：2014-08-08
发明人： Lakmal Jayasinghe , John Hagan Pryce Bayley , Stephen Cheley , Brian McKeown , James White , James Anthony Clarke
CPC分类号： C12Q1/6869 , C07K14/31 , C12N9/1247 , C12N9/1252 , C12N9/127 , C12N9/1276 , C12N9/16 , C12N9/22 , C12N9/52 , C12N9/90 , C12N9/96 , C12Q2565/631
摘要： The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
-
3.发明申请APPARATUS FOR SUPPORTING AN ARRAY OF LAYERS OF AMPHIPHILIC MOLECULES AND METHOD OF FORMING AN ARRAY OF LAYERS OF AMPHIPHILIC MOLECULES 审中-公开用于支持大分子分子层阵列的装置和形成大分子层的阵列的方法公开(公告)号：US20150014160A1
公开(公告)日：2015-01-15
申请号：US14378557
申请日：2013-02-13
IPC分类号： G01N33/487
CPC分类号： G01N33/48721 , B01J2219/00317 , B01J2219/00585 , B01J2219/00599 , B01J2219/00653 , B01J2219/00725 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L3/5088 , B01L2300/0819 , B01L2400/0421 , B01L2400/0427 , B01L2400/0472 , C12Q1/6869 , G01N27/44791 , G01N27/453
摘要： An apparatus for supporting an array of layers of amphiphilic molecules, the apparatus comprising: a body (11), formed in a surface of the body, an array of sensor wells (10) capable of supporting a layer of amphiphilic molecules (30) across the sensor wells, the sensor wells each containing an electrode (12) for connection to an electrical circuit, and formed in the surface of the body between the sensor wells, flow control wells capable of smoothing the flow of a fluid across the surface.
摘要翻译： 一种用于支撑两亲性分子层阵列的装置，所述装置包括：主体（11），形成在所述主体的表面中，能够支撑跨层的两亲分子层（30）的传感器孔阵列（10） 传感器孔，传感器孔各自包含用于连接到电路的电极（12），并且形成在传感器井之间的身体表面中，能够平滑流过表面的流体的流动控制井。
-
公开(公告)号：US09551023B2
公开(公告)日：2017-01-24
申请号：US14427554
申请日：2013-09-06
发明人： Daniel John Turner , Clive Gavin Brown , Stuart William Reid , James Anthony Clarke , James White , David Jackson Stoddart
CPC分类号： C12Q1/6806 , C07H21/04 , C12Q1/6869 , C12Q2525/119 , C12Q2525/301 , C12Q2531/125 , C12Q2533/107 , C12Q2565/631
摘要： The invention relates to a method for modifying a template polynucleotide for characterization, especially for nanopore sequencing. The method produces a modified polynucleotide which is complementary to the template polynucleotide at some positions and which contains universal or abasic nucleotides at the other, and in some instances predicable, positions. The resulting modified polynucleotide can then be characterized.
摘要翻译： 本发明涉及用于修饰模板多核苷酸进行表征的方法，特别是用于纳米孔测序。 该方法产生修饰的多核苷酸，其在某些位置与模板多核苷酸互补，并且在另一个位置包含通用或非碱基核苷酸，并且在某些情况下可以是可预测的位置。 然后可以表征所得修饰的多核苷酸。
-
公开(公告)号：US20160162634A1
公开(公告)日：2016-06-09
申请号：US14346549
申请日：2012-09-21
发明人： Stuart William Reid , Gavin Harper , Clive Gavin Brown , James Anthony Clarke , Andrew John Heron
IPC分类号： G06F19/22 , G01N33/483 , G06F17/18
CPC分类号： G01N33/48721 , B82Y15/00 , C12Q1/6869 , G01N27/44791 , G01N33/483 , G06F17/18 , G06N7/005 , G16B30/00 , C12Q2537/165 , C12Q2565/631
摘要： A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units. In a further embodiment, different voltages are applied across the nanopore during translocation in order to improve the resolution of polymer units.
摘要翻译： 聚合物（3）中的聚合物单元序列，例如 DNA，从与聚合物相关的至少一系列测量估计，例如。 作为通过纳米孔（1）的易位的函数的离子电流，其中每个测量的值取决于作为k个聚合物单元（4）的组的k-mer。 提供概率模型，特别是隐马尔可夫模型（HMM），包括一组可能的k-mers：转换权重，表示从原始k-mers到目的地k-mers的转换机会; 和每个k-mer的发射权重，其表示观察该k-mer的给定值的测量值的机会。 使用分析技术分析一系列测量结果，例如。 维特比解码，其是指模型并且基于通过由聚合物单元的序列产生的一系列测量的模型预测的可能性估计聚合物中的聚合物单元的至少一个估计序列。 在另一个实施方案中，在易位期间跨纳米孔施加不同的电压，以改善聚合物单元的分辨率。
-
公开(公告)号：US20150177237A1
公开(公告)日：2015-06-25
申请号：US14378929
申请日：2013-02-14
发明人： Daniel John Turner , Daniel George Fordham , Roger Charles Gill , Clive Gavin Brown , Stuart Reid , James Anthony Clarke , James White
IPC分类号： G01N33/543 , C12N15/115
摘要： The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.
摘要翻译： 本发明涉及一种在样品中测定两种或更多种分析物组的一种或多种分析物成分的存在或不存在的新方法。 因此，本发明涉及用于确定一组多种分析物中每种分析物的存在或不存在的多重测定。 该测定使用适体和跨膜孔。
-
公开(公告)号：US20210363577A1
公开(公告)日：2021-11-25
申请号：US16972039
申请日：2019-06-06
发明人： James Anthony Clarke , James White , Richard Muscat , Jessica Mary May Johnson , Ramiz Iqbal Nathani , Andrew John Heron , Mark John Bruce , Lakmal Nishantha Jayasinghe , Domenico Caprotti , David Jackson Stoddart , Rebecca Victoria Bowen , Christopher James Wright , Paul Richard Moody
IPC分类号： C12Q1/6869 , G01N33/487
摘要： Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.
-
公开(公告)号：US20210095337A1
公开(公告)日：2021-04-01
申请号：US17064633
申请日：2020-10-07
IPC分类号： C12Q1/6869 , B82Y15/00 , G01N33/487 , G01N27/447
摘要： The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
-
公开(公告)号：US20210087623A1
公开(公告)日：2021-03-25
申请号：US17064628
申请日：2020-10-07
IPC分类号： C12Q1/6869 , B82Y15/00 , G01N33/487 , G01N27/447
摘要： The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
-
公开(公告)号：US10739341B2
公开(公告)日：2020-08-11
申请号：US14378929
申请日：2013-02-14
发明人： Daniel John Turner , Daniel George Fordham , Roger Charles Gill , Clive Gavin Brown , Stuart Reid , James Anthony Clarke , James White
IPC分类号： G01N33/00 , G01N33/543 , G01N33/53 , C12N15/115 , C07H21/04
摘要： The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.
-
-
-
-
-
-
-
-
-
搜索结果
17 条记录 (耗时 0.031 秒)
