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29 条记录 (耗时 0.034 秒)

    Host microorganisms
    21.
    发明授权
    Host microorganisms 失效
    宿主微生物

    公开(公告)号：US07585674B2

    公开(公告)日：2009-09-08

    申请号：US10479214

    申请日：2002-05-28

    申请人： Kazuhisa Sawada ,  Keiji Endo ,  Tadahiro Ozawa ,  Masatoshi Tohata ,  Katsuya Ozaki

    发明人： Kazuhisa Sawada ,  Keiji Endo ,  Tadahiro Ozawa ,  Masatoshi Tohata ,  Katsuya Ozaki

    IPC分类号： C12N5/06 ,  G01N33/53

    CPC分类号： C07K14/32 ,  C07K2319/00 ,  C12N1/20

    摘要： A microorganism wherein one or more genes selected from the group of genes participating in sporulation in the middle to late stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) by use the microorganism. No spore is formed when this microorganism is employed, thereby enabling production of a target product (a protein) while decreasing energy loss, production of a by-product and specific production speed to decrease unnecessary consumption of a medium. Moreover, the production period can be prolonged, whereby the target product (the protein) can be produced efficiently.

    摘要翻译： 一种微生物，其中一个或多个选自参与孢子形成中期至晚期孢子形成的基因的基因已被删除或失活; 以及通过使用微生物生产目标产物（蛋白质）的方法。 当使用该微生物时不形成孢子，从而能够减少能量损失，副产物的产生和特定的生产速度，从而能够生产目标产物（蛋白质），从而减少不必要的培养基消耗。 此外，可以延长生产周期，由此有效地生产目标产物（蛋白质）。

    Modified Promoter
    23.
    发明申请
    Modified Promoter 失效
    修饰的启动子

    公开(公告)号：US20080014608A1

    公开(公告)日：2008-01-17

    申请号：US10589960

    申请日：2005-03-04

    申请人： Keiji Endo ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Katsuya Ozaki

    IPC分类号： C12P21/04 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11

    CPC分类号： C12N15/75 ,  C07K14/32 ,  C12N9/2408 ,  C12N9/2411 ,  C12N9/2417 ,  C12N9/2437

    摘要： The present invention provides a modified promoter DNA capable of enhancing transcription of genes encoding proteins or polypeptides, and a method for producing proteins or polypeptides efficiently by use of the modified promoter DNA.A promoter DNA recognized by SigA and SigE, which is produced by modifying a nucleotide sequence including a promoter recognized by SigA and bases in the vicinity thereof; an expression vector harboring the promoter DNA; a recombinant microorganism containing the expression vector; and a method for producing proteins or polypeptides characterized by culturing the recombinant microorganism.

    摘要翻译： 本发明提供能够增强编码蛋白质或多肽的基因的转录的修饰的启动子DNA，以及通过使用经修饰的启动子DNA有效产生蛋白质或多肽的方法。 由SigA和SigE鉴定的启动子DNA，其通过修饰包括SigA识别的启动子和其附近的碱基的核苷酸序列而产生; 携带启动子DNA的表达载体; 含有表达载体的重组微生物; 以及产生以培养重组微生物为特征的蛋白质或多肽的方法。

    Recombinant Microorganism
    24.
    发明申请
    Recombinant Microorganism 审中-公开
    重组微生物

    公开(公告)号：US20080009039A1

    公开(公告)日：2008-01-10

    申请号：US10578613

    申请日：2004-11-05

    申请人： Masatoshi Tohata ,  Kazuhisa Sawada ,  Katsuya Ozaki ,  Kazuo Kobayashi ,  Naotake Ogasawara

    发明人： Masatoshi Tohata ,  Kazuhisa Sawada ,  Katsuya Ozaki ,  Kazuo Kobayashi ,  Naotake Ogasawara

    IPC分类号： C12P21/04 ,  C12N1/21

    CPC分类号： C12N15/75 ,  C12P21/00

    摘要： A recombinant microorganism obtained by transferring, into a host microorganism capable of producing protein or polypeptide with increased productivity, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism. The recombinant microorganism is prepared by transferring, to a mutant strain of microorganism from which any of Bacillus subtilis genes comA, yopO, treR, yvbA, cspB, yvaN, yttP, yurK, yozA, licR, sigL, mntR, glcT, yvdE, ykvE, sir, rocR, ccpA, yaaT, yyaA, yycH, yacP, hprK, rsiX, yhdK, and yibO, or one or more genes functionally equivalent to any of these genes have been deleted or knocked out, a gene encoding a heterologous protein or polypeptide.

    摘要翻译： 通过将能够产生生产能力的蛋白质或多肽的宿主微生物，编码蛋白质或多肽的基因，以及通过使用重组微生物产生蛋白质或多肽的方法而获得的重组微生物。 通过转移到枯草芽孢杆菌基因comA，yopO，treR，yvbA，cspB，yvaN，yttP，yurK，yozA，licR，sigL，mntR，glcT，yvdE，ykvE中的任一种的微生物突变菌株来制备重组微生物 ，先生，rocR，ccpA，yaaT，yyaA，yycH，yacP，hprK，rsiX，yhdK和yibO，或者与这些基因中的任何一个功能等同的一个或多个基因已被缺失或敲除，编码异源蛋白的基因或 多肽。

    Mutant alpha-amylases
    25.
    发明申请
    Mutant alpha-amylases 审中-公开
    突变型α-淀粉酶

    公开(公告)号：US20050181493A1

    公开(公告)日：2005-08-18

    申请号：US10988529

    申请日：2004-11-16

    申请人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/28 ,  C12N15/56 ,  C11D7/42

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase. The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.

    摘要翻译： 本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶 具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法， 包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。 本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

    Modified promoter
    26.
    发明授权
    Modified promoter 失效
    修饰的启动子

    公开(公告)号：US08623631B2

    公开(公告)日：2014-01-07

    申请号：US10589960

    申请日：2005-03-04

    申请人： Keiji Endo ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Katsuya Ozaki

    IPC分类号： C12N1/20 ,  C12P21/00 ,  C07H21/04

    CPC分类号： C12N15/75 ,  C07K14/32 ,  C12N9/2408 ,  C12N9/2411 ,  C12N9/2417 ,  C12N9/2437

    摘要： The present invention provides a modified promoter DNA capable of enhancing transcription of genes encoding proteins or polypeptides, and a method for producing proteins or polypeptides efficiently by use of the modified promoter DNA.A promoter DNA recognized by SigA and SigE, which is produced by modifying a nucleotide sequence including a promoter recognized by SigA and bases in the vicinity thereof; an expression vector harboring the promoter DNA; a recombinant microorganism containing the expression vector; and a method for producing proteins or polypeptides characterized by culturing the recombinant microorganism.

    摘要翻译： 本发明提供能够增强编码蛋白质或多肽的基因的转录的修饰的启动子DNA，以及通过使用经修饰的启动子DNA有效产生蛋白质或多肽的方法。 由SigA和SigE鉴定的启动子DNA，其通过修饰包括SigA识别的启动子和其附近的碱基的核苷酸序列而产生; 携带启动子DNA的表达载体; 含有表达载体的重组微生物; 以及产生以培养重组微生物为特征的蛋白质或多肽的方法。

    MUTANT ALPHA-AMYLASES
    27.
    发明申请
    MUTANT ALPHA-AMYLASES 审中-公开
    突变型ALPHA-AMYLASES

    公开(公告)号：US20090226961A1

    公开(公告)日：2009-09-10

    申请号：US11864245

    申请日：2007-09-28

    申请人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    IPC分类号： C12P21/00 ,  C07H21/00 ,  C12N15/63 ,  C12N1/00

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase.The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.

    摘要翻译： 本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶 具有所述氨基酸序列的淀粉酶，或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，产生突变型α-淀粉酶的方法， 包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。 本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。