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    Mutant alpha-amylases
    2.
    发明申请
    Mutant alpha-amylases 审中-公开
    突变型α-淀粉酶

    公开(公告)号：US20050181493A1

    公开(公告)日：2005-08-18

    申请号：US10988529

    申请日：2004-11-16

    申请人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/28 ,  C12N15/56 ,  C11D7/42

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase. The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.

    摘要翻译： 本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶 具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法， 包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。 本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

    Mutant α-amylases
    3.
    发明授权
    Mutant α-amylases 失效
    突变型α-淀粉酶

    公开(公告)号：US07078212B1

    公开(公告)日：2006-07-18

    申请号：US09590375

    申请日：2000-06-09

    申请人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    IPC分类号： C12N9/26 ,  C12N9/28 ,  C07H21/04

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase.The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.

    摘要翻译： 本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶 具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法， 包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。 本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

    MUTANT ALPHA-AMYLASES
    6.
    发明申请
    MUTANT ALPHA-AMYLASES 审中-公开
    突变型ALPHA-AMYLASES

    公开(公告)号：US20090226961A1

    公开(公告)日：2009-09-10

    申请号：US11864245

    申请日：2007-09-28

    申请人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    IPC分类号： C12P21/00 ,  C07H21/00 ,  C12N15/63 ,  C12N1/00

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase.The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.

    摘要翻译： 本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶 具有所述氨基酸序列的淀粉酶，或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，产生突变型α-淀粉酶的方法， 包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。 本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

    Recombinant microorganism comprising inactivation of the AprX gene
    9.
    发明授权
    Recombinant microorganism comprising inactivation of the AprX gene 有权
    包含AprX基因失活的重组微生物

    公开(公告)号：US07829322B2

    公开(公告)日：2010-11-09

    申请号：US11722162

    申请日：2005-12-20

    申请人： Takeko Kodama ,  Keiji Endo ,  Katsuya Ozaki ,  Junichi Sekiguchi

    发明人： Takeko Kodama ,  Keiji Endo ,  Katsuya Ozaki ,  Junichi Sekiguchi

    IPC分类号： C12N1/21 ,  C12N15/00 ,  C12N15/57 ,  C12N15/75

    CPC分类号： C12P21/02 ,  C07K14/32 ,  C12N9/54 ,  C12N15/75

    摘要： A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided.Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.

    摘要翻译： 提供能够提高蛋白质或多肽的生产力的宿主微生物，通过将编码蛋白质或多肽的基因导入宿主微生物而获得的重组微生物，以及使用该重组微生物产生蛋白质或多肽的方法。 还提供了通过向宿主微生物中导入编码异源蛋白质或多肽的基因而获得的重组微生物，其中在所述宿主微生物中，枯草芽孢杆菌aprX基因或与aprX基因相对应的基因已被缺失或敲除， 用于使用重组微生物产生蛋白质或多肽。

    Mutant bacterium belonging to the genus bacillus
    10.
    发明申请
    Mutant bacterium belonging to the genus bacillus 有权
    属于杆菌属的突变细菌

    公开(公告)号：US20090170154A1

    公开(公告)日：2009-07-02

    申请号：US10590275

    申请日：2005-03-04

    申请人： Keiji Endo ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Katsuya Ozaki

    IPC分类号： C12P21/00 ,  C12N1/21 ,  C12N15/87

    CPC分类号： C12N9/2417 ,  C12N9/2437 ,  C12N9/54 ,  C12Y302/01004

    摘要： The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.

    摘要翻译： 本发明提供能够提高蛋白质或多肽生产力的突变芽孢杆菌属细菌，通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌产生的重组微生物，以及通过使用重组微生物产生蛋白质或多肽的方法。 一种突变芽孢杆菌属细菌，其在基因组或质粒上具有在孢子形成阶段中特异性识别并转录并与sigA基因或其等同基因相连的基因的上游端的启动子序列的DNA，重组微生物 通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌而产生，以及通过使用重组微生物产生蛋白质或多肽的方法。