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公开(公告)号：US20240076651A1
公开(公告)日：2024-03-07
申请号：US18500232
申请日：2023-11-02
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and fellows of Harvard College
发明人： Feng Zhang , David Benjamin Turitz Cox , Jonathan Gootenberg , Omar O. Abudayyeh , Bernd Zetsche , Jonathan Strecker
CPC分类号： C12N15/102 , A61K48/00 , C12N9/22 , C12N15/11 , C12N15/62 , C12N15/79 , C12N2310/533 , C12Y305/04004
摘要： The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a DNA-targeting Cpf1 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.
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公开(公告)号：US11866697B2
公开(公告)日：2024-01-09
申请号：US16614549
申请日：2018-05-18
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Feng Zhang , David Benjamin Turitz Cox , Jonathan Gootenberg , Omar O. Abudayyeh , Bernd Zetsche , Jonathan Strecker
CPC分类号： C12N15/102 , A61K48/00 , C12N9/22 , C12N15/11 , C12N15/62 , C12N15/79 , C12N2310/533 , C12Y305/04004
摘要： The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a DNA-targeting Cpf1 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.
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公开(公告)号：US20230383282A1
公开(公告)日：2023-11-30
申请号：US18334046
申请日：2023-06-13
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
CPC分类号： C12N15/102 , C12N9/22 , C12N15/111 , C12N15/113 , C12N15/8213 , C12N15/8509 , C12N2310/20 , C12N2800/22
摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.
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公开(公告)号：US11618928B2
公开(公告)日：2023-04-04
申请号：US15952132
申请日：2018-04-12
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Omar Abudayyeh , James Joseph Collins , Jonathan Gootenberg , Feng Zhang , Eric S. Lander , Daniel Neafsey , Angela Early
IPC分类号： C12Q1/6888 , C12N15/11 , C12N9/22 , C12Q1/6883 , C12N15/113
摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.
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公开(公告)号：US20220364071A1
公开(公告)日：2022-11-17
申请号：US17264340
申请日：2019-07-31
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
IPC分类号： C12N9/78 , C12N9/22 , C12N15/11 , C12Q1/6823 , C12Q1/6851
摘要： The present disclosure provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides mutated Cas13 proteins and their use in modifying target sequences as well as mutated Cas13 nucleic acid sequences and vectors encoding mutated Cas13 proteins and vector systems or CRISPR-Cas13 systems.
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公开(公告)号：US20210147915A1
公开(公告)日：2021-05-20
申请号：US17054428
申请日：2019-06-26
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Feng Zhang , Max Kellner , Jonathan Gootenberg , Omar Abudayyeh
IPC分类号： C12Q1/6816
摘要： Provided herein are methods and systems for detecting a target nucleic acid sequence. The method comprises contacting an oligonucleotide comprising the target nucleic acid sequence with a transposon complex; inserting one or more T7 RNA promoters into the oligonucleotide using the transposase; and (c) amplifying the target nucleic acid sequence. The transposon complex may comprise a transposase and a transposon sequence comprising one or more T7 RNA promoters. The target nucleic sequence may be amplified by generating RNA oligonucleotides comprising the target nucleic acid sequence via transcription from the inserted one or more T7 RNA promoters. The amplified target nucleic acid may be detected using a CRISPR Cas13-based detection system.
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27.发明申请公开(公告)号：US20200248169A1
公开(公告)日：2020-08-06
申请号：US16623799
申请日：2018-06-26
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
摘要： The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a RNA-targeting Cas13 protein, at least one guide molecule, and at least one cytidine deaminase protein or catalytic domain thereof.
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公开(公告)号：US20180298445A1
公开(公告)日：2018-10-18
申请号：US15922576
申请日：2018-03-15
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Omar Abudayyeh , James Joseph Collins , Jonathan Gootenberg , Feng Zhang , Eric S. Lander , Aviv Regev
IPC分类号： C12Q1/6883 , C12Q1/6886 , C12N15/11 , C12N9/22 , C12Q1/6888 , C12Q1/689
摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect both DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.
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公开(公告)号：US11788083B2
公开(公告)日：2023-10-17
申请号：US16310577
申请日：2017-06-19
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
CPC分类号： C12N15/102 , C12N9/22 , C12N15/111 , C12N15/113 , C12N15/8213 , C12N15/8509 , C12N2310/20 , C12N2800/22
摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.
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公开(公告)号：US11174515B2
公开(公告)日：2021-11-16
申请号：US15922576
申请日：2018-03-15
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Omar Abudayyeh , James Joseph Collins , Jonathan Gootenberg , Feng Zhang , Eric S. Lander , Aviv Regev
IPC分类号： C12Q1/6883 , C12Q1/6886 , C12N15/11 , C12Q1/6888 , C12Q1/689 , C12N9/22 , C12Q1/6827 , C12Q1/6804
摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect both DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.
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