公开(公告)号：US20200283755A1

公开(公告)日：2020-09-10

申请号：US16614549

申请日：2018-05-18

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Jonathan Gootenberg ,  Omar O. Abudayyeh ,  Bernd Zetsche ,  Jonathan Strecker

IPC分类号： C12N15/10 ,  C12N15/62 ,  C12N9/22

摘要： The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a DNA-targeting Cpf1 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

公开(公告)号：US20240076651A1

公开(公告)日：2024-03-07

申请号：US18500232

申请日：2023-11-02

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and fellows of Harvard College

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Jonathan Gootenberg ,  Omar O. Abudayyeh ,  Bernd Zetsche ,  Jonathan Strecker

IPC分类号： C12N15/10 ,  A61K48/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/62 ,  C12N15/79

CPC分类号： C12N15/102 ,  A61K48/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/62 ,  C12N15/79 ,  C12N2310/533 ,  C12Y305/04004

摘要： The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a DNA-targeting Cpf1 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

公开(公告)号：US11866697B2

公开(公告)日：2024-01-09

申请号：US16614549

申请日：2018-05-18

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Jonathan Gootenberg ,  Omar O. Abudayyeh ,  Bernd Zetsche ,  Jonathan Strecker

IPC分类号： C12N5/10 ,  C12N9/22 ,  C12N15/62 ,  C12N15/10 ,  A61K48/00 ,  C12N15/11 ,  C12N15/79

CPC分类号： C12N15/102 ,  A61K48/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/62 ,  C12N15/79 ,  C12N2310/533 ,  C12Y305/04004

摘要： The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a DNA-targeting Cpf1 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

公开(公告)号：US20230265420A1

公开(公告)日：2023-08-24

申请号：US17928355

申请日：2021-06-18

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Jonathan Strecker ,  Alim Ladha ,  Guilhem Faure

IPC分类号： C12N15/11 ,  C12N15/90 ,  C12N9/22 ,  C12N15/63

CPC分类号： C12N15/11 ,  C12N9/22 ,  C12N15/63 ,  C12N15/90 ,  C12N2310/20

摘要： The present application provides systems, methods and compositions used for targeted gene modification, targeted insertion, perturbation of gene transcripts, nucleic acid editing. Novel nucleic acid targeting systems comprise components of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems and transposable elements.

公开(公告)号：US20240110203A1

公开(公告)日：2024-04-04

申请号：US18270854

申请日：2022-01-07

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Feng Zhang ,  Jonathan Strecker ,  Guilhem Faure

IPC分类号： C12N15/90 ,  C07K14/47 ,  C12N9/22 ,  C12N15/11

CPC分类号： C12N15/907 ,  C07K14/47 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20 ,  C12N2800/90

摘要： The present application provides systems, methods and compositions used for targeted gene modification, targeted insertion, perturbation of gene transcripts, nucleic acid editing. Novel nucleic acid targeting systems comprise components of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems and transposable elements. Specifically, the disclosure provides an engineered composition comprising: a programmable DNA-binding protein and two or more Tn7-like transposition proteins, wherein at least one of the Tn7-like transposition proteins is connected to the DNA-binding protein or otherwise capable of forming a complex with the DNA-binding protein, wherein the DNA-binding protein comprising a Cas protein including a Cas12k protein, and wherein two or more Tn7-like transposition proteins consisting of TnsB, TnsC, and TniQ.

公开(公告)号：US20230049737A1

公开(公告)日：2023-02-16

申请号：US17786168

申请日：2020-12-30

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Jonathan Strecker ,  David Li

IPC分类号： C12N15/90 ,  C12N9/12 ,  C12N9/22

摘要： Systems and methods for targeted gene modification, targeted insertion, perturbation of gene transcripts, and nucleic acid editing. Novel nucleic acid targeting systems comprise components of CRISPR systems, reverse transcriptase, pegRNAs, paired pegRNAs or modified pegRNAs, DNA processing proteins, recombinases, proteins for inhibiting nucleases, and proteins for promoting ssDNA annealing.

公开(公告)号：US20230374551A1

公开(公告)日：2023-11-23

申请号：US18248199

申请日：2021-10-08

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Jonathan Strecker ,  David Li

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N9/12 ,  C12N9/14

CPC分类号： C12N15/907 ,  C12N9/22 ,  C12N15/11 ,  C12N9/1241 ,  C12N9/14 ,  C12Y306/04012 ,  C12N2310/20 ,  C07K2319/80 ,  C12N2800/90

摘要： Compositions, systems and methods for targeted gene modification, insertion and perturbation of gene transcripts and nucleic acid editing are provided. In particular, helitron-mediated gene targeting systems and methods of their use are detailed and provided herein.

公开(公告)号：US11384344B2

公开(公告)日：2022-07-12

申请号：US16717713

申请日：2019-12-17

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Jonathan Strecker ,  Alim Ladha

IPC分类号： C12N9/12 ,  C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/85

摘要： The present application provides systems, methods and compositions used for targeted gene modification, targeted insertion, perturbation of gene transcripts, nucleic acid editing. Novel nucleic acid targeting systems comprise components of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems and transposable elements.

公开(公告)号：US20230383315A1

公开(公告)日：2023-11-30

申请号：US18248252

申请日：2021-10-08

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Makoto Saito ,  Jonathan Strecker

IPC分类号： C12N15/90 ,  C12N15/11 ,  C12N9/22

CPC分类号： C12N15/907 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2800/90

摘要： Systems and methods for targeted gene modification, targeted insertion, perturbation of gene transcripts, and nucleic acid editing. The novel nucleic acid targeting systems can comprise components of one or more transposases, one or more components of a CRISPR-Cas system, and a transposable element.

公开(公告)号：US20230193242A1

公开(公告)日：2023-06-22

申请号：US16772222

申请日：2018-12-21

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Bernd Zetsche ,  Jonathan Strecker

IPC分类号： C12N15/10 ,  C12N15/113 ,  C12N9/22

CPC分类号： C12N15/102 ,  C12N9/22 ,  C12N15/113 ,  C07K2319/09 ,  C12N2310/20

摘要： Embodiments herein include engineered CRISPR-Cas effector proteins that comprise at least one modification compared to an unmodified CRISPR-Cas effector protein (e.g., C2c1) that enhances binding of the of the CRISPR complex to the binding site and/ or alters editing preference as compared to wild type. Embodiments disclosed further include viral vectors for delivery of CRISPR-Cas effector proteins. The vectors may be designed to allow packaging of the CRISPR-Cas effector protein within a single vector. Certain embodiments further include delivery vectors, constructs, and methods of delivering larger genes for systemic delivery.