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公开(公告)号:US5831011A
公开(公告)日:1998-11-03
申请号:US590554
申请日:1996-03-21
申请人: Jewel Payne , Kenneth E. Narva , Jenny Fu
发明人: Jewel Payne , Kenneth E. Narva , Jenny Fu
IPC分类号: C12N15/09 , A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325 , C12N5/10 , C12N15/31 , C07K14/32 , C12N1/20
CPC分类号: A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325 , Y10S435/832
摘要: Novel B.t. genes encoding toxins active against nematode pests have been cloned. The DNA encoding the B.t. toxin can be used to transform various hosts to express the B.t. toxin.
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公开(公告)号:US5635480A
公开(公告)日:1997-06-03
申请号:US455313
申请日:1995-05-31
IPC分类号: C12N15/09 , A01N63/00 , A61K38/00 , C07K14/325 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/19
CPC分类号: A01N63/00 , C07K14/325 , C12R1/075 , A61K38/00
摘要: Disclosed and claimed are toxins produced by novel Bacillus thuringiensis isolates designated B.t. PS92J, B.t. PS196S1, B.t. PS201L1, and B.t. PS201T6, which have dipteran and/or corn rootworm activity. Thus, the isolates, or mutants thereof, can be used to control such pests. Further, claimed are novel genes encoding these .delta.-endotoxins, which can be expressed in other hosts. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.
摘要翻译: 披露和要求的是由命名为B.t.的新型苏云金芽孢杆菌分离株产生的毒素。 PS92J,B.t. PS196S1,B.t. PS201L1和B.t. PS201T6,其具有dipteran和/或玉米根虫活性。 因此,分离物或其突变体可用于控制这些害虫。 此外,要求的是编码这些δ-内毒素的新基因,其可以在其他宿主中表达。 这种宿主中的δ-内毒素的表达导致对这种宿主环境中易感害虫的控制。
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23.
公开(公告)号:US5632987A
公开(公告)日:1997-05-27
申请号:US357698
申请日:1994-12-16
申请人: Jewel Payne , Kenneth E. Narva
发明人: Jewel Payne , Kenneth E. Narva
IPC分类号: A01N63/00 , C07K14/325
CPC分类号: A01N63/00 , C07K14/325
摘要: Disclosed and claimed are toxins and genes from Bacillus thuringiensis strains designated PS80JJ1, PS158D5, PS167P, PS169E, PS177F1, PS177G, PS204G4, PS204G6 which can be used to control corn rootworm. Mutants which retain the activity of the parent strain can be used to control the pest. Further, isolated spores or purified toxins from these isolates can be used to control corn rootworm. Genes encoding .delta.-endotoxins can be removed from these strains using standard well-known techniques, and transferred to other hosts. Expression of the .delta.-endotoxin in such hosts results in control of corn rootworm larvae.
摘要翻译: 披露和要求的是来自苏云金芽孢杆菌菌株的命名为PS80JJ1,PS158D5,PS167P,PS169E,PS177F1,PS177G,PS204G4,PS204G6的毒素和基因,其可以用于控制玉米根虫。 保留亲本菌株活性的突变体可用于防治害虫。 此外,这些分离物的分离的孢子或纯化的毒素可用于控制玉米根虫。 可以使用标准的熟知技术从这些菌株中去除编码δ-内毒素的基因,并转移到其他宿主。 这种宿主中的δ-内毒素的表达导致玉米根虫幼虫的控制。
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公开(公告)号:US5322932A
公开(公告)日:1994-06-21
申请号:US97798
申请日:1993-07-27
IPC分类号: A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325 , C07K15/04
CPC分类号: A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325 , C12R1/075
摘要: A novel B.t. gene encoding a toxin which is toxic to nematodes has been cloned from a B. thuringiensis microbe. The DNA encoding the B.t. toxin can be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These recombinant microbes can be used to control nematodes in various environments.
摘要翻译: 小说B.t. 从苏云金芽孢杆菌微生物克隆了对线虫有毒的毒素基因。 编码B.t.的DNA 毒素可用于转化各种原核和真核微生物,以表达B.t. 毒素。 这些重组微生物可用于控制各种环境中的线虫。
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公开(公告)号:US5262399A
公开(公告)日:1993-11-16
申请号:US675772
申请日:1991-03-27
CPC分类号: C12R1/075 , A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325
摘要: Methods and compositions for the control of flukes are described. Specifically, Bacillus thuringiensis (B.t.) isolates having flukicidal activity are disclosed. Also described are recombinant hosts which express B.t. genes coding for flukicidal toxins. The B.t. isolates and recombinant proteins are shown to be useful in a method for controlling flukes including the liver fluke.
摘要翻译: 描述了用于控制吸虫的方法和组合物。 具体来说,公开了具有氟杀菌活性的苏云金芽孢杆菌(B.t.)分离株。 还描述了表达B.t.的重组宿主。 编码流感毒素的基因。 B.t. 分离物和重组蛋白质显示可用于控制包括肝吸虫在内的吸虫的方法。
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26.
公开(公告)号:US07129212B2
公开(公告)日:2006-10-31
申请号:US10698096
申请日:2003-10-31
申请人: Kenneth E. Narva , Donald J. Merlo
发明人: Kenneth E. Narva , Donald J. Merlo
CPC分类号: C12Q1/689 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要: Disclosed and claimed are novel Bacillus thuringiensis isolates, pesticidal toxins, and genes. The subject invention also provides novel methods of controlling diamond back moths.
摘要翻译: 公开和要求的是新型苏云金芽孢杆菌分离株,杀虫毒素和基因。 本发明还提供了控制钻石背蛾的新方法。
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27.
公开(公告)号:US06673990B2
公开(公告)日:2004-01-06
申请号:US09826660
申请日:2001-04-05
IPC分类号: A01H500
CPC分类号: C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要: The subject invention provides a DNA construct comprising a promoter operably linked to a nucleic acid encoding an N-terminal core toxin and a C-terminal Cry1Ab protoxin portion, wherein the nucleic acid encoding the C-terminal Cry1Ab protoxin portion is a plant optimized polynucleotide sequence.
摘要翻译: 本发明提供了包含与编码N末端核心毒素和C-末端Cry1Ab原毒素部分的核酸可操作连接的启动子的DNA构建体,其中编码C末端Cry1Ab原毒素部分的核酸是植物优化的多核苷酸序列 。
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公开(公告)号:US06605701B2
公开(公告)日:2003-08-12
申请号:US09967805
申请日:2001-09-28
申请人: H. Ernest Schnepf , Kenneth E. Narva , Brian A. Stockhoff , Stacey Finstad Lee , Mikki Walz , Blake Sturgis
发明人: H. Ernest Schnepf , Kenneth E. Narva , Brian A. Stockhoff , Stacey Finstad Lee , Mikki Walz , Blake Sturgis
IPC分类号: C07H2102
CPC分类号: C07K14/32 , C07K14/245
摘要: Disclosed and claimed are novel toxins and genes obtainable from Bacillus laterosporus isolates disclosed herein. In preferred embodiments, the subject genes and toxins are used to control Western corn rootworm.
摘要翻译: 公开并要求保护的是可从本文公开的芽孢杆菌分离株获得的新型毒素和基因。 在优选的实施方案中,主题基因和毒素用于控制西方玉米根虫。
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公开(公告)号:US06274721B1
公开(公告)日:2001-08-14
申请号:US09456830
申请日:1999-12-07
IPC分类号: C07H2104
CPC分类号: C07K14/325 , C07K2319/00 , C12N15/8286 , C12N2799/026 , Y02A40/162 , Y10T436/143333
摘要: Disclosed and claimed are novel methods for controlling lepidopteran pests whereby said pests are contacted with a pesticidal amount of a toxin obtainable from Bacillus thuringiensis isolate PS31G1 (NRRL B-21560).
摘要翻译: 公开和要求的是控制鳞翅目害虫的新方法,其中所述害虫与可从苏云金芽孢杆菌分离株PS31G1(NRRL B-21560)获得的杀虫剂量的毒素接触。
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30.
公开(公告)号:US06242669B1
公开(公告)日:2001-06-05
申请号:US09073898
申请日:1998-05-06
申请人: Jerald S. Feitelson , H. Ernest Schnepf , Kenneth E. Narva , Brian A. Stockhoff , James Schmeits , David Loewer , Charles Joseph Dullum , Judy Muller-Cohn , Lisa Stamp , George Morrill , Stacey Finstad-Lee
发明人: Jerald S. Feitelson , H. Ernest Schnepf , Kenneth E. Narva , Brian A. Stockhoff , James Schmeits , David Loewer , Charles Joseph Dullum , Judy Muller-Cohn , Lisa Stamp , George Morrill , Stacey Finstad-Lee
IPC分类号: A01H500
CPC分类号: C12N15/8286 , A01N63/00 , C07K14/325 , C12Q1/689 , C12Q2600/142 , C12Q2600/156 , Y02A40/162
摘要: Disclosed and claimed are novel Bacillus thuringiensis isolates, pesticidal toxins, genes, and nucleotide probes and primers for the identification of genes encoding toxins active against pests. The primers are useful in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins. The subject invention provides entirely new families of toxins from Bacillus isolates.
摘要翻译: 公开和要求的是新型苏云金芽孢杆菌分离株,杀虫毒素,基因和核苷酸探针和引物,用于鉴定编码针对害虫的毒素的基因。 引物可用于PCR技术以产生编码这些毒素的基因特征的基因片段。 本发明提供了来自分离芽孢杆菌的全新的毒素家族。
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