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公开(公告)号：US11174500B2
公开(公告)日：2021-11-16
申请号：US16551340
申请日：2019-08-26
Applicant: Translate Bio, Inc.
Inventor： Joseph Parella , Kimberly Gillis , Jonathan Abysalh , Travis Jeannotte , Erik Held , Frank DeRosa , Michael Heartlein , Rebecca Powell
Abstract: The present invention provides, among other things, methods for purifying mRNA based on normal flow filtration for therapeutic use.
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公开(公告)号：US20210324369A1
公开(公告)日：2021-10-21
申请号：US17172527
申请日：2021-02-10
Applicant: Translate Bio, Inc.
Inventor： Jonathan Abysalh , Jorel Vargas , Cameron M. Smith , Joseph Parrella , Frank DeRosa
IPC: C12N15/10
Abstract: The present invention provides, among other things, methods for purifying high quality messenger (mRNA) suitable for clinical use. The present invention is, in part, based on surprising discovery that capping and tailing mRNA in reaction buffer having a pH lower than 8.0 and MgCl2 at a concentration of less than 1.25 mM can increase RNA integrity of final mRNA product. Thus, the present invention provides an effective, reliable, and efficient method of manufacturing high quality RNA at large scale for therapeutic use.
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公开(公告)号：US12084702B2
公开(公告)日：2024-09-10
申请号：US17450506
申请日：2021-10-11
Applicant: Translate Bio, Inc.
Inventor： Joseph Parella , Kimberly Gillis , Jonathan Abysalh , Travis Jeannotte , Erik Held , Frank DeRosa , Michael Heartlein , Rebecca Powell
CPC classification number: C12P19/34 , B01D25/00 , B01D37/00 , C07H1/06 , C07H21/02 , C07H21/04 , C12N15/1017 , C12N15/52 , C12N2330/30
Abstract: The present invention provides, among other things, methods for purifying mRNA based on normal flow filtration for therapeutic use.
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公开(公告)号：US11976272B2
公开(公告)日：2024-05-07
申请号：US17070877
申请日：2020-10-14
Applicant: Translate Bio, Inc.
Inventor： Jonathan Abysalh , Daniel Crawford , Frank DeRosa , Shrirang Karve , Anusha Dias , Michael Heartlein
IPC: C12N15/10 , B01L3/00 , C12Q1/6806
CPC classification number: C12N15/1017 , B01L3/502753 , C12Q1/6806 , B01L2200/028 , B01L2200/16 , B01L2300/0681 , B01L2400/0409 , C12Q2523/113 , C12Q2523/308 , C12Y201/03003
Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.
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Patent Agency Ranking
公开(公告)号：US20230062449A1
公开(公告)日：2023-03-02
申请号：US17871121
申请日：2022-07-22
Applicant: Translate Bio, Inc.
Inventor： Jonathan Abysalh , Frank DeRosa , Cameron M. Smith , Jorel E. Vargas
IPC: C12N15/10
Abstract: The present invention provides, among other things, methods for purifying high quality messenger RNA (mRNA) suitable for clinical use, without using any caustic or flammable solvents. The present invention is, in part, based on surprising discovery that mRNA can be successfully purified by selective precipitation and washing without using ethanol while maintaining integrity and high purity of mRNA. Thus, the present invention provides an effective, reliable, and safer method of purifying RNA from large scale manufacturing process therapeutic applications without using any caustic or inflammable solvents.
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公开(公告)号：US11560562B2
公开(公告)日：2023-01-24
申请号：US15907163
申请日：2018-02-27
Applicant: Translate Bio, Inc.
Inventor： Anusha Dias , Daniel Crawford , Frank DeRosa , Jonathan Abysalh , Michael Heartlein
IPC: C12N15/10 , C12P19/34 , C12N9/12 , G01N27/447
Abstract: The present invention provides methods for large-scale production of a composition enriched for full-length mRNA molecules using an SP6 RNA polymerase and compositions produced using such methods and uses thereof.
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公开(公告)号：US20220170061A1
公开(公告)日：2022-06-02
申请号：US17450506
申请日：2021-10-11
Applicant: Translate Bio, Inc.
Inventor： Joseph Parella , Kimberly Gillis , Jonathan Abysalh , Travis Jeannotte , Erik Held , Frank DeRosa , Michael Heartlein , Rebecca Powell
Abstract: The present invention provides, among other things, methods for purifying mRNA based on normal flow filtration for therapeutic use.
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公开(公告)号：US11173190B2
公开(公告)日：2021-11-16
申请号：US15981757
申请日：2018-05-16
Applicant: Translate Bio, Inc.
Inventor： Michael Heartlein , Frank DeRosa , Alan Kimura , Jonathan Abysalh , Anusha Dias , Shrirang Karve , Zarna Patel
Abstract: The present invention provides, among other things, methods of treating cystic fibrosis, comprising a step of administering to a subject in need of treatment a composition comprising an mRNA encoding a Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein, wherein the mRNA encoding the CFTR protein comprises a polynucleotide sequence at least 80% identical to SEQ ID NO: 1, wherein the mRNA is at a concentration of at least 0.4 mg/mL, and wherein the step of administering comprises inhalation.
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公开(公告)号：US20200095571A1
公开(公告)日：2020-03-26
申请号：US16551340
申请日：2019-08-26
Applicant: Translate Bio, Inc.
Inventor： Joseph Parella , Kimberly Gillis , Jonathan Abysalh , Travis Jeannotte , Erik Held , Frank DeRosa , Michael Heartlein , Rebecca Powell
IPC: C12N15/10
Abstract: The present invention provides, among other things, methods for purifying mRNA based on normal flow filtration for therapeutic use.
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