利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

41 条记录 (耗时 0.038 秒)

    Generation of Chemical Building Blocks from Plant Biomass by Selective Depolymerization
    23.
    发明申请
    Generation of Chemical Building Blocks from Plant Biomass by Selective Depolymerization 有权
    通过选择性解聚从植物生物量生成化学构件

    公开(公告)号：US20090004697A1

    公开(公告)日：2009-01-01

    申请号：US12138096

    申请日：2008-06-12

    申请人： Andre Koltermann ,  Ulrich Kettling ,  Thomas Bruck ,  Markus Rarbach

    发明人： Andre Koltermann ,  Ulrich Kettling ,  Thomas Bruck ,  Markus Rarbach

    IPC分类号： C12P19/00

    CPC分类号： C12N9/2434 ,  C12N9/0061 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14 ,  C12P19/16 ,  C12P19/18 ,  C12P19/20 ,  C12P19/22 ,  C12P19/24 ,  C13B35/005 ,  C13K1/02 ,  C13K1/06 ,  C13K13/002

    摘要： The invention concerns a method for the enzymatic treatment of raw polymeric feedstock comprising the following steps: (a) preferably separation of soluble components from the raw polymeric feedstock, (b) treating the raw polymeric feedstock with an enzyme system in order to liberate defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock; and (c) separating the defined monomeric or oligomeric building blocks produced in step b) from the remainder of the raw polymeric feedstock. Preferably, the enzyme system used in step b) contains not more than 50%, preferably not more than 20%, more preferably not more than 10%, more preferably not more than 5%, more preferably not more than 2%, more preferably not more than 1% of other enzyme activities apart from the enzyme activity resulting in liberation of said defined monomeric or oligomeric building blocks from the raw polymeric feedstock according to step b). Further aspects of the invention concern the use of “less pure” and thus less costly enzyme systems in subsequent enzymatic treatment steps and methods for determining the optimum sequence of enzymatic treatment steps by analysis of the raw polymeric feedstock used.

    摘要翻译： 本发明涉及用于酶处理原料聚合物原料的方法，包括以下步骤：（a）优选将可溶性组分与原料聚合原料分离，（b）用酶体系处理原料聚合物原料，以释放限定的可溶性 来自不溶性原料聚合原料的单体或低聚结构单元; 和（c）将步骤b）中制备的限定的单体或低聚结构单元与原始聚合原料的其余部分分离。 优选地，步骤b）中使用的酶系统含有不超过50％，优选不超过20％，更优选不超过10％，更优选不超过5％，更优选不超过2％ 不超过1％的其它酶活性，除了酶活性，导致根据步骤b）从所述原料聚合物原料释放所述限定的单体或低聚结构单元。 本发明的另外方面涉及在随后的酶处理步骤中使用“较不纯”且因此较低成本的酶体系，以及通过分析所用原料聚合物原料来确定酶处理步骤的最佳顺序的方法。

    Method for the production of biopolymers with modified properties
    24.
    发明授权
    Method for the production of biopolymers with modified properties 有权
    生产具有改性特性的生物聚合物的方法

    公开(公告)号：US06821758B1

    公开(公告)日：2004-11-23

    申请号：US09708497

    申请日：2000-11-09

    申请人： Andre Koltermann ,  Ulrich Kettling ,  Manfred Eigen

    发明人： Andre Koltermann ,  Ulrich Kettling ,  Manfred Eigen

    IPC分类号： C12P1934

    CPC分类号： C12N15/1027

    摘要： A method for the production of biopolymers with modified properties wherein at least one cycle comprising the following steps is completed: (a) providing a population of single-stranded polynucleotide molecules, wherein individual polynucleotide molecules comprise homologous and heterologous sequence segments and wherein individual ones of said single-stranded polynucleotide molecules can form double-stranded polynucleotide molecules with other ones of said single-stranded polynucleotide molecules within said population; (b) forming double-stranded polynucleotide molecules from the population of single-stranded polynucleotide molecules provided according to step (a) comprising double-stranded polynucleotide molecules with different heterologous sequence segments; (c) partially and exonucleolytically degrading the single-strands of the double-stranded polynucleotide molecules produced according to step (b); and (d) temple-directed single-stand synthesizing the degraded ends of the partially degraded double strand produced according to step (c), wherein steps (c) and (d) may be carried out sequentially or contemporaneously.

    摘要翻译： 一种用于生产具有改进性质的生物聚合物的方法，其中完成包括以下步骤的至少一个循环：（a）提供一群单链多核苷酸分子，其中单个多核苷酸分子包含同源和异源序列区段， 所述单链多核苷酸分子可以与所述群体内的所述单链多核苷酸分子中的其它单链多核苷酸分子形成双链多核苷酸分子;（b）从根据步骤（I）提供的单链多核苷酸分子群体形成双链多核苷酸分子 a）包含具有不同异源序列片段的双链多核苷酸分子;（c）部分和外核解离降解根据步骤（b）制备的双链多核苷酸分子的单链; 和（d）合成根据步骤（c）制备的部分降解的双链的降解末端的神经导向单支，其中步骤（c）和（d）可以顺序或同时进行。

    Method and device for multicolour 2-photon fluorescence coincidence analysis
    29.
    发明授权
    Method and device for multicolour 2-photon fluorescence coincidence analysis 有权
    多色双光子荧光重合分析方法与装置

    公开(公告)号：US07507582B2

    公开(公告)日：2009-03-24

    申请号：US10333034

    申请日：2001-07-18

    申请人： Katrin Heinze ,  Petra Schwille ,  Andre Koltermann ,  Ulrich Kettling

    发明人： Katrin Heinze ,  Petra Schwille ,  Andre Koltermann ,  Ulrich Kettling

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6408 ,  B82Y10/00 ,  B82Y20/00 ,  G01J2003/2866 ,  G01N2021/6441 ,  Y10T436/13

    摘要： A method of fluorescence measurement on analytes, marked with different fluorescent markers, which have spectrally different fluorescence emissions, in a sample is described, having the following steps: illumination of the sample in the measurement volume using a laser to excite the fluorescence emission of the at least two fluorescent markers, the illumination of the measurement volume being performed with a maximum of one single laser line, having an excitation intensity sufficiently high that the fluorescent markers are jointly excited through 2-photon absorptions, detection of the fluorescence emission using at least two detector devices which are designed for light detection in different spectral ranges in accordance with the spectral fluorescence properties of the fluorescent markers, and performance of a cross-correlation and/or coincidence analysis of detector signals of the detector devices. A measurement device for performing the method is also described.

    摘要翻译： 描述了在样品中具有不同荧光标记的分析物上荧光测量的方法，所述分析物具有不同的荧光发射，具有以下步骤：使用激光照射测量体积中的样品以激发荧光发射 至少两个荧光标记物，用最大量的一条单一激光线照射测量体积，具有足够高的激发强度，使得荧光标记物通过双光子吸收共同激发，至少使用至少检测荧光发射 两个检测器装置被设计用于根据荧光标记的光谱荧光特性在不同光谱范围内进行光检测，以及执行检测器装置的检测器信号的互相关和/或重合分析。 还描述了用于执行该方法的测量装置。

    Multi-parameter fluorimetric analysis in a massively parallel multi-focal arrangement and the use thereof
    30.
    发明授权
    Multi-parameter fluorimetric analysis in a massively parallel multi-focal arrangement and the use thereof 失效
    多参数荧光分析在大规模并行多焦点布置中的应用及其应用

    公开(公告)号：US07170598B2

    公开(公告)日：2007-01-30

    申请号：US10688101

    申请日：2003-10-17

    申请人： Peter Jomo Walla ,  Andre Koltermann ,  Ulrich Kettling ,  Mathias Scharte

    发明人： Peter Jomo Walla ,  Andre Koltermann ,  Ulrich Kettling ,  Mathias Scharte

    IPC分类号： G01J3/30

    CPC分类号： G01J3/4406 ,  G01N21/6408 ,  G01N21/6456 ,  G01N21/718 ,  G01N2021/178 ,  G01N2021/1789

    摘要： A method for analyzing properties of a sample by measuring fluorescence parameters in multiple foci, comprises the steps of splitting a collimated primary laser beam with a splitting device into at least two collimated secondary laser beams and deflecting the secondary laser beams such that they propagate at different propagation angles with respect to an optical axis of a focussing optic, focussing the secondary laser beams with the focussing optic into at least two volume elements in the sample, detecting light emitted from the volume elements with a detecting device, and evaluating the detected light for obtaining the properties to be analyzed. Furthermore, a device for implementing this method is described. The invention is particularly suited for high-throughput screening applications.

    摘要翻译： 通过测量多个焦点中的荧光参数来分析样品的性质的方法包括以下步骤：将具有分裂装置的准直主激光束分成至少两个准直的次级激光束并使次级激光束偏转，使得它们在不同的 相对于聚焦光学器件的光轴的传播角度，将具有聚焦光学器件的次级激光束聚焦到样品中的至少两个体积元件中，用检测装置检测从体积元件发射的光，并且将检测到的光用于 获得待分析的属性。 此外，描述了用于实现该方法的装置。 本发明特别适用于高通量筛选应用。