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    PROCESS FOR THE ENZYMATIC PRODUCTION OF C4 COMPOUNDS FROM C6 SUBSTRATES
    4.
    发明申请
    PROCESS FOR THE ENZYMATIC PRODUCTION OF C4 COMPOUNDS FROM C6 SUBSTRATES 审中-公开
    从C6底物生产C4化合物的方法

    公开(公告)号：US20140171683A1

    公开(公告)日：2014-06-19

    申请号：US14006847

    申请日：2012-03-26

    申请人： Volker Sieber ,  André Pick ,  Broder Rühmann

    发明人： Volker Sieber ,  André Pick ,  Broder Rühmann

    IPC分类号： C12P7/18

    CPC分类号： C12P7/18 ,  C12P7/16 ,  C12P13/001 ,  Y02E50/10

    摘要： The present invention relates to a novel process for converting a substrate of formula (III) and/or (IV) into a product of formula (I) or (II) comprising the following reactions: a) oxidation of at least one terminal C-atom, b) dehydratation, c) decarboxylation and d) reduction and/or amination. At least step b is enzyme-catalyzed. In a preferred embodiment, all reactions are enzymatically catalyzed. The enzymes catalyzing the reactions are selected from oxidoreductases, decarboxylases, dehydratases and/or aminotransferases. The process may be performed in a cell-free in vitro production system or in an improved fermentative production system.

    摘要翻译： 本发明涉及将式（III）和/或（IV）的底物转化为式（I）或（II）的产物的新方法，其包括以下反应：a）至少一个末端C- 原子，b）脱水，c）脱羧和d）还原和/或胺化。 至少步骤b是酶催化的。 在优选的实施方案中，所有反应都被酶促催化。 催化反应的酶选自氧化还原酶，脱羧酶，脱水酶和/或氨基转移酶。 该方法可以在无细胞的体外生产系统中或在改进的发酵生产系统中进行。

    Method for producing gene libraries
    7.
    发明申请
    Method for producing gene libraries 审中-公开
    生产基因文库的方法

    公开(公告)号：US20060003324A1

    公开(公告)日：2006-01-05

    申请号：US10468391

    申请日：2002-02-11

    申请人： Volker Sieber

    发明人： Volker Sieber

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12N15/1093 ,  C12N15/102

    摘要： The presented invention refers to a method for the creation of gene libraries wherein a defined number of adjacent nucleotides is exchanged and gene libraries are produced which code for protein variants having more manifold amino acid exchanges and a more homogenous distribution of mutations than can be obtained using conventional methods. DNA-strands are incorporated at random positions into a gene of interest. Then parts of the donor strands and parts of the gene sequence that is flanking these strands are removed, however, a defined number (e.g. 3) of nucleotides that originate from the donor strand remain in the gene at the place of a defined number (e.g. 3) of nucleotides of the original gene having been removed from it. Combined with a selection step after the incorporation of the donor strand into the gene it can be ensured that the nucleotides to be exchanged/introduced are in a specific reading frame. When the nucleotides of the donor strand that remain in the genes are degenerate, gene libraries can be produced with variants that have any codon at any position.

    摘要翻译： 本发明涉及一种用于产生基因文库的方法，其中定义数量的相邻核苷酸被交换，并且产生编码具有更多多肽氨基酸交换的蛋白质变体和更均匀的突变分布的基因文库，其可以使用 常规方法。 DNA链在随机位置并入感兴趣的基因。 然后去除部分供体链和位于这些链侧翼的基因序列的部分，然而，起源于供体链的定义数目（例如3）的核苷酸保留在基因中定义数目的位置（例如， 3）原始基因的核苷酸已被除去。 结合供体链融入基因后的选择步骤，可以确保待交换/引入的核苷酸在特定阅读框中。 当保留在基因中的供体链的核苷酸是退化的时，可以用在任何位置具有任何密码子的变体产生基因文库。

    Process for the enzymatic production of C4 compounds from C6 substrates
    10.
    发明授权
    Process for the enzymatic production of C4 compounds from C6 substrates 有权
    从C6底物酶催化生产C4化合物的方法

    公开(公告)号：US09169500B2

    公开(公告)日：2015-10-27

    申请号：US14006847

    申请日：2012-03-26

    申请人： Volker Sieber ,  André Pick ,  Broder Rühmann

    发明人： Volker Sieber ,  André Pick ,  Broder Rühmann

    IPC分类号： C07C59/00 ,  C12P7/18 ,  C12P7/16 ,  C12P13/00

    CPC分类号： C12P7/18 ,  C12P7/16 ,  C12P13/001 ,  Y02E50/10

    摘要： The present invention relates to a novel process for converting a substrate of formula (III) and/or (IV) into a product of formula (I) or (II) comprising the following reactions: a) oxidation of at least one terminal C-atom, b) dehydratation, c) decarboxylation and d) reduction and/or amination. At least step b is enzyme-catalyzed. In a preferred embodiment, all reactions are enzymatically catalyzed. The enzymes catalyzing the reactions are selected from oxidoreductases, decarboxylases, dehydratases and/or aminotransferases. The process may be performed in a cell-free in vitro production system or in an improved fermentative production system.

    摘要翻译： 本发明涉及将式（III）和/或（IV）的底物转化成式（I）或（II）的产物的新方法，其包括以下反应：a）至少一个末端C- 原子，b）脱水，c）脱羧和d）还原和/或胺化。 至少步骤b是酶催化的。 在优选的实施方案中，所有反应都被酶促催化。 催化反应的酶选自氧化还原酶，脱羧酶，脱水酶和/或氨基转移酶。 该方法可以在无细胞的体外生产系统中或在改进的发酵生产系统中进行。