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1.发明授权公开(公告)号：US07507582B2
公开(公告)日：2009-03-24
申请号：US10333034
申请日：2001-07-18
IPC分类号： G01N21/64
CPC分类号： G01N21/6408 , B82Y10/00 , B82Y20/00 , G01J2003/2866 , G01N2021/6441 , Y10T436/13
摘要： A method of fluorescence measurement on analytes, marked with different fluorescent markers, which have spectrally different fluorescence emissions, in a sample is described, having the following steps: illumination of the sample in the measurement volume using a laser to excite the fluorescence emission of the at least two fluorescent markers, the illumination of the measurement volume being performed with a maximum of one single laser line, having an excitation intensity sufficiently high that the fluorescent markers are jointly excited through 2-photon absorptions, detection of the fluorescence emission using at least two detector devices which are designed for light detection in different spectral ranges in accordance with the spectral fluorescence properties of the fluorescent markers, and performance of a cross-correlation and/or coincidence analysis of detector signals of the detector devices. A measurement device for performing the method is also described.
摘要翻译： 描述了在样品中具有不同荧光标记的分析物上荧光测量的方法，所述分析物具有不同的荧光发射，具有以下步骤：使用激光照射测量体积中的样品以激发荧光发射 至少两个荧光标记物，用最大量的一条单一激光线照射测量体积，具有足够高的激发强度，使得荧光标记物通过双光子吸收共同激发，至少使用至少检测荧光发射 两个检测器装置被设计用于根据荧光标记的光谱荧光特性在不同光谱范围内进行光检测，以及执行检测器装置的检测器信号的互相关和/或重合分析。 还描述了用于执行该方法的测量装置。
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公开(公告)号：US20050112682A9
公开(公告)日：2005-05-26
申请号：US10343977
申请日：2001-08-07
IPC分类号： A01K67/033 , C07K14/435 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12P21/02 , C12Q1/37 , G01N21/64 , G01N21/78 , G01N33/573 , G01N33/58 , C12Q1/70 , C07H21/04 , C12N5/06 , C12P21/04 , G01N33/53
CPC分类号： C12Q1/37 , G01N33/582
摘要： The invention concerns an autofluorescent fusion protein suitable for use as a protease substrate, a nucleic acid sequence which encodes this fusion protein, and a method of using the fusion protein and/or the nucleic acid sequence in a dual color, confocal fluorometric assay for the detection and quantification of proteolytic activity or proteolytic inhibitory activity in samples or cells.
摘要翻译： 本发明涉及适合用作蛋白酶底物的自发荧光融合蛋白，编码该融合蛋白的核酸序列，以及在双色共焦荧光测定法中使用融合蛋白和/或核酸序列的方法， 检测和定量样品或细胞中的蛋白水解活性或蛋白水解抑制活性。
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公开(公告)号：US06200818B1
公开(公告)日：2001-03-13
申请号：US09581217
申请日：2000-06-22
申请人： Manfred Eigen , Thorsten Winkler , Jens Stephan , Petra Schwille , Andre Koltermann , Ulrich Kettling , Klaus Dörre , Jan Bieschke
发明人： Manfred Eigen , Thorsten Winkler , Jens Stephan , Petra Schwille , Andre Koltermann , Ulrich Kettling , Klaus Dörre , Jan Bieschke
IPC分类号： G01N2164
CPC分类号： G01N21/6408 , G01N2021/6421
摘要： The invention relates to a method for detecting reactions and conformational changes of analytes in a sample by coincidence analysis, as follows: the sample is marked with at least two different fluorescent dyes and then illuminated with at least one laser in order to stimulate the emission of fluorescence; the fluorescence signals are detected by at least two detection units and each signal is broken down into any simultaneous time segments with the desired time slot widths; the number of signals contained in at least one time segment and/or the time intervals between signals in the time segments are detected; a coincidence analysis of the data detected is carried out for at least one time segment of the first detection unit with at least one isochronous time segment of the second detection unit; statistics are produced showing the results of the coincidence analysis and/or the results are subjected to a threshold value analysis, and these statistics or a combination of more statistics are evaluated for the presence of characteristic features.
摘要翻译： 本发明涉及通过重合分析检测样品中分析物的反应和构象变化的方法，如下：样品用至少两种不同的荧光染料标记，然后用至少一种激光照射，以刺激样品的发射 荧光; 荧光信号由至少两个检测单元检测，并且每个信号被分解成具有所需时隙宽度的任何同步时间段; 检测包含在至少一个时间段中的信号数量和/或时间段中的信号之间的时间间隔; 对于具有第二检测单元的至少一个等时时间段的第一检测单元的至少一个时间段执行检测到的数据的一致性分析; 产生显示符合分析的结果和/或对结果进行阈值分析的统计量，并且对特征特征的存在来评估这些统计量或更多统计量的组合。
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4.发明授权Thermostable xylanase for the selective hydrolysis of pentose-containing polysaccharides 有权用于选择性水解含糖的多糖的热稳定的木聚糖酶公开(公告)号：US08679795B2
公开(公告)日：2014-03-25
申请号：US13517344
申请日：2010-12-15
申请人： Andreas Kohl , Isabel Unterstrasser , Markus Rarbach , Andre Koltermann , Christoph Reisinger , Thomas Brück , Ulrich Kettling , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson
发明人： Andreas Kohl , Isabel Unterstrasser , Markus Rarbach , Andre Koltermann , Christoph Reisinger , Thomas Brück , Ulrich Kettling , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson
IPC分类号： C12N9/24
CPC分类号： C12N9/248
摘要： The present invention relates to polypeptides having xylanase activity and nucleic acid sequences encoding such polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. One specific application of the xylanase is the selective hydrolysis of pentose sugar components of hemicellulose-containing plant biomass. The nucleotide sequence may be used for the production of the xylanase or optimized mutants thereof.
摘要翻译： 本发明涉及具有木聚糖酶活性的多肽和编码此类多肽的核酸序列。 本发明还涉及包含核酸构建体的核酸构建体，载体和宿主细胞以及用于产生和使用多肽的方法。 木聚糖酶的一个具体应用是含有半纤维素的植物生物质的戊糖糖成分的选择性水解。 核苷酸序列可用于生产木聚糖酶或其优化的突变体。
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公开(公告)号：US20120301578A1
公开(公告)日：2012-11-29
申请号：US13232588
申请日：2011-09-14
申请人： Andrei Miasnikov , Vijay Kumar , Oliver Kensch , Klaus Pellangahr , Birgitta Leuthner , Ulrich Kettling , Andre Koltermann
发明人： Andrei Miasnikov , Vijay Kumar , Oliver Kensch , Klaus Pellangahr , Birgitta Leuthner , Ulrich Kettling , Andre Koltermann
CPC分类号： C12N9/16
摘要： The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.
摘要翻译： 本发明涉及酶和方法。 特别地，描述了分离的多肽，其包含对应于弗氏柠檬酸杆菌植酸酶的氨基酸序列或其同源物，修饰形式，功能等同物或其有效片段。 还描述了用编码细菌植酸酶或修饰形式的核酸转化或转染的宿主细胞，以及在食物或动物饲料中使用这种植酸酶或修饰形式。
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公开(公告)号：US20120135475A1
公开(公告)日：2012-05-31
申请号：US13322395
申请日：2010-05-21
申请人： Andre Koltermann , Markus Rarbach , Thomas Bruck , Jochen Gerlach , Isabel Unterstrasser , Andreas Kohl , Zdravko Dragovic , Ulrich Kettling
发明人： Andre Koltermann , Markus Rarbach , Thomas Bruck , Jochen Gerlach , Isabel Unterstrasser , Andreas Kohl , Zdravko Dragovic , Ulrich Kettling
摘要： The invention is directed to a liquefied sugar beet and/or sugar cane biomass material as well as production methods and uses thereof. The liquefied biomass is storage stable and can be used for the production of a product resulting from fermentation.
摘要翻译： 本发明涉及液化甜菜和/或甘蔗生物质材料及其生产方法和用途。 液化生物质储存稳定，可用于生产发酵产物。
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公开(公告)号：US20090074925A1
公开(公告)日：2009-03-19
申请号：US11696162
申请日：2007-04-03
申请人： Andrei Miasnikov , Vijay Kumar , Oliver Kensch , Klaus Pellangahr , Birgitta Leuthner , Ulrich Kettling , Andre Koltermann
发明人： Andrei Miasnikov , Vijay Kumar , Oliver Kensch , Klaus Pellangahr , Birgitta Leuthner , Ulrich Kettling , Andre Koltermann
CPC分类号： C12N9/16
摘要： The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.
摘要翻译： 本发明涉及酶和方法。 特别地，描述了分离的多肽，其包含对应于弗氏柠檬酸杆菌植酸酶的氨基酸序列或其同源物，修饰形式，功能等同物或其有效片段。 还描述了用编码细菌植酸酶或修饰形式的核酸转化或转染的宿主细胞，以及在食物或动物饲料中使用这种植酸酶或修饰形式。
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8.发明申请公开(公告)号：US20060147909A1
公开(公告)日：2006-07-06
申请号：US10478809
申请日：2002-05-31
CPC分类号： C12N15/1079 , B01L3/502753 , B01L3/502784 , B01L2200/0673 , B01L2300/0864 , B01L2400/0633 , B01L2400/0661
摘要： The invention relates to microstructures and the use thereof for the directed evolution of biomolecules.
摘要翻译： 本发明涉及微结构及其在生物分子定向进化中的应用。
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公开(公告)号：US20090208440A1
公开(公告)日：2009-08-20
申请号：US11969511
申请日：2008-01-04
申请人： Ulrich Haupts , Andre Koltermann , Andreas Scheidig , Christian Votsmeier , Ulrich Kettling , Oliver Kensch , Birgitta Leuthner
发明人： Ulrich Haupts , Andre Koltermann , Andreas Scheidig , Christian Votsmeier , Ulrich Kettling , Oliver Kensch , Birgitta Leuthner
CPC分类号： C12N9/6424 , A61K8/64 , A61K38/482 , A61K38/4826 , A61K38/486 , A61K38/4873 , A61K38/488 , A61Q19/00 , C12N9/50 , C12N9/6472 , C12N9/6478 , C12N9/6489 , C12N15/1034 , C12N15/62 , C12P21/06 , C12Q1/37
摘要： The present invention provides engineered enzymes generated from protein scaffolds combined with Specificity Determining Regions, the production thereof and the use of said engineered enzymes for research, nutritional care, personal care and industrial purposes.
摘要翻译： 本发明提供由与特异性确定区组合的蛋白质支架产生的工程化酶，其生产和所述工程化酶用于研究，营养保健，个人护理和工业目的的用途。
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公开(公告)号：US20070298145A1
公开(公告)日：2007-12-27
申请号：US11737057
申请日：2007-04-18
申请人： Andrei MIASNIKOV , Vijay Kumar , Oliver Kensch , Klaus Pellengahr , Birgitta Leuthner , Ulrich Kettling , Andre Koltermann
发明人： Andrei MIASNIKOV , Vijay Kumar , Oliver Kensch , Klaus Pellengahr , Birgitta Leuthner , Ulrich Kettling , Andre Koltermann
IPC分类号： A23K1/165 , C12N1/15 , C12N1/19 , C12N1/21 , C12N15/55 , C12N15/63 , C12N15/70 , C12N15/74 , C12N15/75 , C12N9/16
CPC分类号： C12N9/16
摘要： The present invention relates to enzymes and processes. In particular, there is described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme and variants thereof.
摘要翻译： 本发明涉及酶和方法。 特别地，描述了用编码细菌植酸酶及其变体的核酸转化或转染的宿主细胞。
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