公开(公告)号：US20220356512A1

公开(公告)日：2022-11-10

申请号：US17813415

申请日：2022-07-19

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. CHIU ,  Jason E. KREUTZ ,  Gloria S. YEN ,  Bryant S. FUJIMOTO

IPC: C12Q1/6816 ,  G01N15/14 ,  G06V10/50 ,  G06V20/69 ,  G06T7/62 ,  G01N21/47 ,  G01N21/64

Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

公开(公告)号：US20200206740A1

公开(公告)日：2020-07-02

申请号：US16639083

申请日：2018-08-15

Applicant: UNIVERSITY OF WASHINGTON ,  MICAREO INC.

Inventor： Daniel T. CHIU ,  Mengxia ZHAO ,  Eleanor S. JOHNSON ,  Perry G. SCHIRO ,  Hui Min YU ,  Wei-Feng FANG ,  Jui-Lin CHEN

IPC: B01L3/00 ,  G01N15/14 ,  G01N33/49

Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and can be contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

公开(公告)号：US20200158724A1

公开(公告)日：2020-05-21

申请号：US16702228

申请日：2019-12-03

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. CHIU ,  Wei SUN ,  Jiangbo YU ,  Changfeng WU ,  Fangmao YE

IPC: G01N33/543 ,  G01N33/58 ,  C09K11/02 ,  C08G61/02 ,  C08G73/00 ,  C08G75/32 ,  G01N33/533

Abstract: Lyophilized chromophoric polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions, methods of dispersing the lyophilized compositions in aqueous solutions and kits supplying the compositions.

公开(公告)号：US20190234953A1

公开(公告)日：2019-08-01

申请号：US16209729

申请日：2018-12-04

Applicant: University of Washington ,  Clemson University

Inventor： Daniel T. CHIU ,  Changfeng WU ,  Jason MCNEILL ,  Jiangbo YU

IPC: G01N33/58 ,  C09K11/06

CPC classification number: G01N33/587 ,  C09K11/06 ,  G01N33/582

Abstract: The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.

公开(公告)号：US20190106542A1

公开(公告)日：2019-04-11

申请号：US16138732

申请日：2018-09-21

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. CHIU ,  Changfeng WU ,  Yu RONG ,  Yong ZHANG ,  Yi-Che WU ,  Yang-Hsiang CHAN ,  Xuanjun ZHANG ,  Jiangbo YU ,  Wei SUN

IPC: C08G75/32 ,  C09B69/10 ,  H01L51/00 ,  A61K49/00 ,  C08G79/00 ,  G01N33/58

Abstract: Polymers, monomers, chromophoric polymer dots and related methods are provided. Highly fluorescent chromophoric polymer dots with narrow-band emissions are provided. Methods for synthesizing the chromophoric polymers, preparation methods for forming the chromophoric polymer dots, and biological applications using the unique properties of narrow-band emissions are also provided.