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公开(公告)号：US20200157615A1
公开(公告)日：2020-05-21
申请号：US16783037
申请日：2020-02-05
Inventor： Daniel T. CHIU , Bryant S. FUJIMOTO , Alexander R. GANSEN , Gloria S. YEN , Robert M. LORENZ
IPC: C12Q1/6851 , C12Q1/6806
Abstract: Methods and systems for digital measurements are provided. In an embodiment, the method includes producing a plurality of droplets, wherein at least one of the droplets of the plurality of droplets contains an analyte molecule from a sample; measuring at least a first portion of the plurality of droplets to determine individual volumes of droplets in the first portion of the plurality of droplets; analyzing at least a second portion of the plurality of droplets to determine a number of droplets in the second portion of the plurality of droplets that contain the analyte molecule; and using individual volumes of the droplets in the first portion of the plurality of droplets and the number of droplets in the second portion of the plurality of droplets that contain the analyte molecule to determine the concentration of the analyte molecule in the sample.
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公开(公告)号：US20170175174A1
公开(公告)日：2017-06-22
申请号：US15301798
申请日：2015-04-08
Inventor： Daniel T. CHIU , Jason E. KREUTZ , Gloria S. YEN , Bryant S. FUJIMOTO
CPC classification number: C12Q1/6816 , G01N15/1434 , G01N21/47 , G01N21/6428 , G01N2015/1445 , G01N2021/6439 , G06K9/0014 , G06K9/4647 , G06T7/62 , G06T2207/10056 , G06T2207/30072 , C12Q2531/113 , C12Q2537/157 , C12Q2563/107 , C12Q2563/159
Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.
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公开(公告)号：US20160086785A1
公开(公告)日：2016-03-24
申请号：US14891620
申请日：2014-05-30
Inventor： Daniel T. CHIU , Bejan HAKIMI , Dingsheng LIU , Frantisek TURECEK , Michael VOLNY , Joelle ROLFS
CPC classification number: H01J49/165 , B05B5/025 , B05B5/03 , H01J49/0445
Abstract: The present disclosure describes devices and methods capable of generating multi-phase emulsions, including double emulsion droplets in a gas phase. The present disclosure also describes interfaces for coupling a multi-phase emulsion droplet source to an analytical instrument such as a mass spectrometer. The present disclosure further describes methods, systems, and apparatuses for using the devices and interfaces described to perform analysis, including mass spectrometry. The present disclosure also describes methods, systems, and apparatuses for generating and using multi-phase emulsions to perform analysis.
Abstract translation: 本公开描述了能够产生多相乳液的装置和方法，包括气相中的双乳液液滴。 本公开还描述了用于将多相乳液液滴源耦合到诸如质谱仪的分析仪器的界面。 本公开进一步描述了使用所描述的装置和接口进行分析的方法，系统和装置，包括质谱法。 本公开还描述了用于产生和使用多相乳液进行分析的方法，系统和装置。
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公开(公告)号：US20160354777A1
公开(公告)日：2016-12-08
申请号：US14900926
申请日：2014-06-25
Inventor： Daniel T. CHIU , Thomas SCHNEIDER , Jason E. KREUTZ
CPC classification number: B01L3/50273 , B01L3/50851 , B01L7/52 , B01L2200/0605 , B01L2200/0642 , B01L2200/0673 , B01L2300/0627 , B01L2300/0803 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487
Abstract: Devices, systems and apparatuses for the discretization and manipulation of sample volumes are provided. Related methods are also provided.
Abstract translation: 提供了用于离散化和操纵样品体积的装置，系统和装置。 还提供了相关方法。
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5.发明申请HYBRID SEMICONDUCTING POLYMER NANOPARTICLES AS POLARIZATION-SENSITIVE FLUORESCENT PROBES 审中-公开混合型半导体聚合物纳米粒子作为偏振敏感荧光探针公开(公告)号：US20160161475A1
公开(公告)日：2016-06-09
申请号：US14903801
申请日：2014-07-08
Inventor： Daniel T. CHIU , Maxwell ZEIGLER
IPC: G01N33/543 , C09K11/06
CPC classification number: G01N33/54346 , B82Y30/00 , C08G2261/124 , C08G2261/1412 , C08G2261/3142 , C08G2261/3246 , C08G2261/522 , C08G2261/94 , C08L65/00 , C08L2205/02 , C09K11/06 , C09K2211/1416 , C09K2211/1425 , C09K2211/1483 , G01N33/587 , C08L25/18 , C08L23/36
Abstract: Compositions of, methods of making, and methods of using hybrid nanoparticles comprise at least one semiconducting polymer and at least one nonsemiconducting polymer. Compositions of, methods of making and methods of using hybrid nanoparticles comprise at least one semiconducting polymer and non-semiconducting polymers wherein the non-semiconducting polymer comprises more than one non-semiconducting polymer such that at least one non-semiconducting polymer is functionalized for bioconjugation. The hybrid nanoparticles are polarization-sensitive and have low mass ratios with large fluorescence.
Abstract translation: 制备方法和使用混合纳米颗粒的方法的组合物包含至少一种半导体聚合物和至少一种非半导体聚合物。 制备方法和使用混合纳米颗粒的方法的组合物包括至少一种半导体聚合物和非半导体聚合物，其中非半导体聚合物包含多于一种非半导体聚合物，使得至少一种非半导体聚合物被官能化用于生物共轭 。 杂化纳米颗粒是极化敏感的，并且具有低质量比和大的荧光。
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Patent Agency Ranking
公开(公告)号：US20160018405A1
公开(公告)日：2016-01-21
申请号：US14772608
申请日：2014-03-05
Inventor： Daniel T. CHIU
IPC: G01N33/58
CPC classification number: G01N33/588 , B82Y15/00 , B82Y40/00 , G01N33/6803 , G01N2458/30 , Y10S977/774 , Y10S977/92
Abstract: Methods, systems, compositions and kits are provided for the analysis of target molecules using chromophoric polymer dots conjugated to biomolecules. The use of chromophoric polymer dots improves detection sensitivity and stability when compared with existing techniques. In some aspects, methods, systems, and kits are provided for detecting a target protein using chromophoric polymer dots conjugated to biomolecules in a Western blot analysis. Related methods, systems, compositions and kits are also provided.
Abstract translation: 提供了使用与生物分子缀合的发色聚合物点分析靶分子的方法，系统，组合物和试剂盒。 与现有技术相比，使用发色聚合物点提高了检测灵敏度和稳定性。 在一些方面，提供了用于在蛋白质印迹分析中使用与生物分子缀合的发色聚合物点检测靶蛋白的方法，系统和试剂盒。 还提供了相关方法，系统，组合物和试剂盒。
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公开(公告)号：US20230023536A1
公开(公告)日：2023-01-26
申请号：US17932687
申请日：2022-09-16
Inventor： Daniel T. CHIU , Mengxia ZHAO , Wyatt NELSON , Perry G. SCHIRO
Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.
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公开(公告)号：US20200174005A1
公开(公告)日：2020-06-04
申请号：US16702176
申请日：2019-12-03
Inventor： Daniel T. CHIU , Mengxia ZHAO , Wyatt NELSON , Perry G. SCHIRO
Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.
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公开(公告)号：US20190004056A1
公开(公告)日：2019-01-03
申请号：US16041569
申请日：2018-07-20
Inventor： Daniel T. CHIU , Yuhui JIN , Fangmao YE , Changfeng WU , Yang-Hsiang CHAN
Abstract: Polymer nanoparticles and related methods are provided. The polymer particles can include polymer dots having a coating including a polyelectrolyte polymer. Methods of making and using the polymer nanoparticles are also provided.
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公开(公告)号：US20180251827A1
公开(公告)日：2018-09-06
申请号：US15962944
申请日：2018-04-25
Inventor： Daniel T. CHIU , Bryant S. FUJIMOTO , Alexander R. GANSEN , Gloria S. YEN , Robert M. LORENZ
IPC: C12Q1/6851
CPC classification number: C12Q1/6851 , C12Q1/6806 , C12Q2527/146 , C12Q2531/113 , C12Q2545/114
Abstract: Methods, devices and systems for performing digital measurements are provided.
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