公开(公告)号：US20090189063A1

公开(公告)日：2009-07-30

申请号：US12330374

申请日：2008-12-08

申请人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

发明人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

IPC分类号： H01J49/00

CPC分类号： G01N33/6851 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004

摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.

摘要翻译： 在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。 迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。 关于已经测量的蛋白质和源自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据高精度对照以确定同位素峰。 以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 包含在MSn谱中的信息在涉及多级解离和质谱（MSn）的MSn的每个步骤中被有效地利用，使得用于确定下一个分析内容的流程和为母体离子的选择 例如，MSn + 1分析可以在实时测量和高效率和精确度下进行优化。 因此，用户关注的目标可以进行串联质谱，无需浪费测量。

公开(公告)号：US20080073504A1

公开(公告)日：2008-03-27

申请号：US11675173

申请日：2007-02-15

申请人： Kenichi Shinbo ,  Fujio Oonishi ,  Ritsuro Orihashi ,  Yasushi Terui ,  Tsukasa Shishika

发明人： Kenichi Shinbo ,  Fujio Oonishi ,  Ritsuro Orihashi ,  Yasushi Terui ,  Tsukasa Shishika

IPC分类号： H01J49/00

CPC分类号： H01J49/0036

摘要： In a data acquisition system of ADC system, a log amplifier is provided at the pre-stage of an A/D converter, a signal amplified by the log amplifier having a nonlinear input-output characteristic is A/D-converted, and an adding operation of data is performed while reconverting a voltage value data which is converted to a nonlinear characteristic to data with a linear scale according to a table memory for reverse-log conversion. A known voltage value is inputted into the log amplifier to perform measurement, and calibration of the table memory is performed by storing the voltage value and the voltage value data after A/D-converted.

摘要翻译： 在ADC系统的数据采集系统中，在A / D转换器的前级提供对数放大器，由具有非线性输入输出特性的对数放大器放大的信号进行A / D转换， 执行数据的操作，同时根据用于反向对数转换的表存储器，将转换成非线性特性的电压值数据重新转换成具有线性刻度的数据。 已知的电压值被输入到对数放大器中以进行测量，并且通过在A / D转换后存储电压值和电压值数据来执行表存储器的校准。

公开(公告)号：US06765200B2

公开(公告)日：2004-07-20

申请号：US09792582

申请日：2001-02-23

申请人： Akira Maekawa ,  Akira Owada ,  Yasushi Terui

发明人： Akira Maekawa ,  Akira Owada ,  Yasushi Terui

IPC分类号： H01J4910

CPC分类号： H01J49/105

摘要： In order to provide an equipment that permits easy elimination of interference ions even in a low resolution mass spectrometer, the isotope mass number information and the isotope presence ratio information for each element are stored, and processing is done as is shown in FIG. 1 using such information and the measurement results, and the amount of target elemental ions is calculated by calculating and eliminating the amount of interference caused by other elemental isotopes to the target element. Because of this, when carrying out element analysis using a mass spectrometer with a relatively low resolution, the user can easily obtain the measurement result of the amount of any target elemental ions.

摘要翻译： 为了提供即使在低分辨率质谱仪中也容易消除干扰离子的设备，存储每个元件的同位素质量数信息和同位素存在比信息，并且如图1所示进行处理。 使用这样的信息和测量结果，并且通过计算和消除由其他元素同位素对目标元素引起的干扰量来计算目标元素离子的量。 因此，当使用分辨率相对较低的质谱仪进行元素分析时，用户可以容易地获得任何目标元素离子的量的测量结果。

公开(公告)号：US08797522B2

公开(公告)日：2014-08-05

申请号：US13701399

申请日：2011-07-01

申请人： Akihiro Namba ,  Fujio Onishi ,  Yasushi Terui

发明人： Akihiro Namba ,  Fujio Onishi ,  Yasushi Terui

IPC分类号： G01J1/44 ,  G01J1/42

CPC分类号： G01J1/44 ,  G01J1/10 ,  G01J1/42

摘要： To enable measurement over a wide dynamic range from weak light quantity to strong light quantity in a light quantity detection device for detecting the light quantity, a detection signal from a photon counting light detector is A/D converted. When the A/D converted detection signal has a preset threshold value or more, the detection signal is transmitted as it is to a number-of-photons calculation circuit in a subsequent stage, and when the detection signal has the threshold value or less, threshold value processing for transmitting a preset reference value to the subsequent stage is performed. In the number-of-photons calculation circuit, the number of photons or the light quantity incident on the photon counting light detector is acquired from the dimension of an acquired detection signal waveform until the light quantity measurement ends.

摘要翻译： 为了在用于检测光量的光量检测装置中的弱光量到强光量的宽动态范围内进行测量，来自光子计数光检测器的检测信号被A / D转换。 当A / D转换的检测信号具有预设的阈值或更大时，检测信号被原样发送到下一级中的光子数计算电路，并且当检测信号具有阈值或更小时， 执行用于将预设参考值发送到后级的阈值处理。 在光子数计算电路中，从所获取的检测信号波形的尺寸获取入射到光子计数光检测器上的光子数或光量直到光量测量结束。

公开(公告)号：US08633841B2

公开(公告)日：2014-01-21

申请号：US13387122

申请日：2010-08-30

申请人： Fujio Oonishi ,  Yasushi Terui ,  Tsukasa Shishika

发明人： Fujio Oonishi ,  Yasushi Terui ,  Tsukasa Shishika

IPC分类号： H03M1/62

CPC分类号： H01J49/025

摘要： A signal processing device includes amplifiers that are capable of amplifying detected signals using amplification factors that are different from each other; A/D converters that sample plural signals amplified by the amplifiers using the different amplification factors and output from the amplifiers; calculators that perform, on the basis of the amplification factors of the plural amplifiers, calculation on plural data pieces converted by the A/D converters; and a selector that selects one or more of output data pieces from among plural data pieces output from the calculators.

摘要翻译： 信号处理装置包括能够使用彼此不同的放大因子来放大检测信号的放大器; A / D转换器，其采用不同的放大因子对放大器放大的多个信号进行采样并从放大器输出; 基于多个放大器的放大系数执行由A / D转换器转换的多个数据片的计算的计算器; 以及从计算器输出的多个数据中选择一个或多个输出数据段的选择器。

公开(公告)号：US20130114073A1

公开(公告)日：2013-05-09

申请号：US13701399

申请日：2011-07-01

申请人： Akihiro Namba ,  Fujio Onishi ,  Yasushi Terui

发明人： Akihiro Namba ,  Fujio Onishi ,  Yasushi Terui

IPC分类号： G01J1/44

CPC分类号： G01J1/44 ,  G01J1/10 ,  G01J1/42

摘要： To enable measurement over a wide dynamic range from weak light quantity to strong light quantity in a light quantity detection device for detecting the light quantity, a detection signal from a photon counting light detector is A/D converted. When the A/D converted detection signal has a preset threshold value or more, the detection signal is transmitted as it is to a number-of-photons calculation circuit in a subsequent stage, and when the detection signal has the threshold value or less, threshold value processing for transmitting a preset reference value to the subsequent stage is performed. In the number-of-photons calculation circuit, the number of photons or the light quantity incident on the photon counting light detector is acquired from the dimension of an acquired detection signal waveform until the light quantity measurement ends.

摘要翻译： 为了在用于检测光量的光量检测装置中的弱光量到强光量的宽动态范围内进行测量，来自光子计数光检测器的检测信号被A / D转换。 当A / D转换的检测信号具有预设的阈值或更大时，检测信号被原样发送到下一级中的光子数计算电路，并且当检测信号具有阈值或更小时， 执行用于将预设参考值发送到后级的阈值处理。 在光子数计算电路中，从所获取的检测信号波形的尺寸获取入射到光子计数光检测器上的光子数或光量直到光量测量结束。

公开(公告)号：US07999222B2

公开(公告)日：2011-08-16

申请号：US12474992

申请日：2009-05-29

申请人： Tsukasa Shishika ,  Yasushi Terui

发明人： Tsukasa Shishika ,  Yasushi Terui

IPC分类号： H01J49/00

CPC分类号： H01J49/025 ,  H01J49/40

摘要： A time-of-flight mass spectrometer includes a detector and is adapted to measure the time it takes for an accelerated ion to reach the detector and thereby measure the mass of the ion. The time-of-flight mass spectrometer scans a voltage applied to an ion incident side surface of the detector in accordance with a mass to be measured. An electrode is provided between the detector and a space in which an ion flies. The time-of-flight mass spectrometer is capable of measuring ions of a wide range of masses with high detection efficiency by scanning a voltage applied to the electrode.

摘要翻译： 飞行时间质谱仪包括检测器，并且适于测量加速离子到达检测器所需的时间，从而测量离子的质量。 飞行时间质谱仪根据要测量的质量扫描施加到检测器的离子入射侧表面的电压。 在检测器和离子飞行的空间之间设置电极。 飞行时间质谱仪通过扫描施加到电极的电压，能够以高检测效率测量宽范围的质量离子。

公开(公告)号：US20110192970A1

公开(公告)日：2011-08-11

申请号：US13090009

申请日：2011-04-19

申请人： Fujio Oonishi ,  Kenichi Shinbo ,  Ritsuro Orihashi ,  Yasushi Terui ,  Tsukasa Shishika

发明人： Fujio Oonishi ,  Kenichi Shinbo ,  Ritsuro Orihashi ,  Yasushi Terui ,  Tsukasa Shishika

IPC分类号： H01J49/40

CPC分类号： H01J49/0036 ,  H01J49/40

摘要： For the achievement of data transfer time reduction, removal of noise data, and analytical efficiency improvement in an ADC data processing function of a time-of-flight mass spectrometer, the mass spectrometer comprises a data acquisition circuit including: an ND converter; a signal intensity addition memory that stores data of ion signals such as a time range and the number of measurements and performs an addition process; a voltage value frequency addition memory that performs an addition process of frequencies of voltage values of the predetermined time range and the number of measurements and stores addition results; a threshold level computation circuit that computes a predetermined threshold level from the results in the memory; a compression memory that extracts only data exceeding the threshold level from the data in the signal intensity addition memory; and a counter that controls a measurement time for data acquisition and the operation of each circuit.

摘要翻译： 为了实现数据传输时间的减少，噪声数据的去除以及飞行时间质谱仪的ADC数据处理功能的分析效率改进，质谱仪包括数据采集电路，包括：ND转换器; 信号强度相加存储器，其存储诸如时间范围和测量次数的离子信号的数据，并执行加法处理; 电压值频率相加存储器，其执行预定时间范围的电压值的频率和测量次数的加法处理，并存储加法结果; 阈值电平计算电路，用于根据存储器中的结果计算预定的阈值电平; 压缩存储器，其从信号强度相加存储器中的数据中仅提取超过阈值电平的数据; 以及控制数据采集和每个电路的操作的测量时间的计数器。

公开(公告)号：US07932486B2

公开(公告)日：2011-04-26

申请号：US12330374

申请日：2008-12-08

申请人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

发明人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

IPC分类号： H01J49/26

CPC分类号： G01N33/6851 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004

摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.

摘要翻译： 在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。 迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。 关于已经测量的蛋白质和来自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据高精度对照以确定同位素峰。 以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 包含在MSn谱中的信息在涉及多级解离和质谱（MSn）的MSn的每个步骤中被有效地利用，使得用于确定下一个分析内容的流程和为母体离子的选择 例如，MSn + 1分析可以在实时测量和高效率和精确度下进行优化。 因此，用户关注的目标可以进行串联质谱，无需浪费测量。

公开(公告)号：US07755035B2

公开(公告)日：2010-07-13

申请号：US11889264

申请日：2007-08-10

申请人： Hiroshi Nakamura ,  Tsukasa Shishika ,  Yasushi Terui ,  Takuya Saeki

发明人： Hiroshi Nakamura ,  Tsukasa Shishika ,  Yasushi Terui ,  Takuya Saeki

IPC分类号： H01J49/40

CPC分类号： H01J49/42 ,  H01J49/401

摘要： An ion trap time-of-flight mass spectrometer capable of obtaining highly-sensitive mass spectra even on the lower mass number side is realized. The ion trap time-of-flight mass spectrometer includes an ion source that operates at atmospheric pressure, an ion optical system for introducing the ions generated by the ion source into a vacuum chamber and converging the ions introduced into the vacuum chamber, an ion trap part for trapping ions in the vacuum chamber, a multipole part for converging the kinetic energy of the ions discharged from the ion trap, and a time-of-flight mass spectrometry part for measuring the ions discharged from the multipole part. The period of high-voltage pulses generated by an electrode provided in the time-of-flight mass spectrometry part can be changed depending on an ion content introduced into the multipole part.

摘要翻译： 实现了即使在较低质量数侧也可获得高灵敏度质谱的离子阱飞行时间质谱仪。 离子阱飞行时间质谱仪包括在大气压下工作的离子源，离子光学系统，用于将由离子源产生的离子引入真空室并会聚进入真空室的离子，离子阱 在真空室中捕获离子的部分，用于会聚从离子阱排出的离子的动能的多极部分和用于测量从多极部分排出的离子的飞行时间质谱部分。 通过设置在飞行时间质谱部分中的电极产生的高电压脉冲的周期可以根据引入多极部分的离子含量而改变。