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公开(公告)号：US5763162A
公开(公告)日：1998-06-09
申请号：US161231
申请日：1993-12-02
IPC分类号： C07D221/12 , C07D405/12 , C07D417/06 , C07D417/14 , C09B23/04 , C12Q1/68 , G01N27/447 , G01N27/26
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , G01N27/44726 , G01N27/44747 , Y10T436/143333
摘要： Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
摘要翻译： 提供新的荧光标记技术和荧光标记，采用高亲和力非共价结合和插入荧光染料和dsDNA。 染料可用于提供电泳凝胶中核酸的高灵敏度标记，以及预先制备的用于结合多种特异性结合对成员的标记。 DNA染料荧光剂复合物可用于诊断测定中的标记，特异性核酸序列的检测等。
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32.发明授权DNA complexes with dyes designed for energy transfer as fluorescent markers 失效DNA复合物与设计用于能量转移的染料作为荧光标记物
公开(公告)号：US5646264A
公开(公告)日：1997-07-08
申请号：US340749
申请日：1995-01-23
IPC分类号： G01N33/58 , C07D221/12 , C07D405/12 , C07D417/06 , C07D417/14 , C07H21/04 , C09B23/04 , C12N15/09 , C12Q1/68 , G01N27/447 , C07H21/00 , C07H19/04
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , C12Q1/6818 , G01N27/44726 , G01N27/44747
摘要： Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.
摘要翻译： 提供了异源多聚体荧光团用于结合DNA，其允许在电分离中检测DNA并制备具有高荧光效率和大的斯托克斯位移的探针。 此外，通过适当选择荧光分子，可以使用单个窄波长带激发光源，同时获得具有足够分离的荧光发射以易于鉴别。
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公开(公告)号：US5312921A
公开(公告)日：1994-05-17
申请号：US60910
申请日：1993-05-14
IPC分类号： G01N33/533 , C07D215/38 , C07D221/12 , C07D405/12 , C07D417/06 , C07D417/14 , C07H21/04 , C09B23/04 , C09B69/00 , C09K11/06 , C12N15/09 , C12Q1/68 , G01N27/447
CPC分类号： C07D417/06 , C07D221/12 , C07D405/12 , C07D417/14 , C09B23/04 , C12Q1/6816 , G01N27/44726
摘要： Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
摘要翻译： 提供了新的荧光染料，其特征在于具有连接至至少两个正电荷的聚阳离子链的荧光团。 与没有聚阳离子链的荧光团相比，发现这些染料提供与核酸结合的高度增强并且与核酸具有强的结合亲和力。 该染料可用于使用基本上较少染料的凝胶电泳和溶液中以显着更高的灵敏度检测dsDNA。
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公开(公告)号：US4542104A
公开(公告)日：1985-09-17
申请号：US483006
申请日：1983-04-06
申请人： Lubert Stryer , Alexander N. Glazer
发明人： Lubert Stryer , Alexander N. Glazer
IPC分类号： G01N33/533 , G01N33/542 , G01N33/52
CPC分类号： G01N33/542 , G01N33/533 , Y10S436/80
摘要： Fluorescent conjugates are employed providing combinations of a fluorescent sensitizer and a fluorescent phycobiliprotein. The conjugates find use in applications where large Stokes shifts, high absorption coefficients and high fluorescence quantum yields are desired. Particularly, combinations of phycobiliproteins are employed where the wavelength of excitation may be 50 nm or more different from the emission wavelength.
摘要翻译： 使用荧光共轭物提供荧光增感剂和荧光藻胆蛋白的组合。 共轭物可用于需要大斯托克斯位移，高吸收系数和高荧光量子产率的应用中。 特别地，使用藻红蛋白的组合，其中激发波长可以与发射波长不同50nm或更大。
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