中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

67 records (took 0.061 seconds)

    METHODS OF GENE EXPRESSION MONITORING
    1.
    发明申请
    METHODS OF GENE EXPRESSION MONITORING 审中-公开
    基因表达监测方法

    公开(公告)号：US20060292614A1

    公开(公告)日：2006-12-28

    申请号：US11467971

    申请日：2006-08-29

    Applicant: Yanxiang Cao ,  Lubert Stryer ,  David Lockhart ,  Catherine Dulac ,  Ian Tietjen ,  Jason Rihel

    Inventor： Yanxiang Cao ,  Lubert Stryer ,  David Lockhart ,  Catherine Dulac ,  Ian Tietjen ,  Jason Rihel

    IPC: C12Q1/68

    CPC classification number: C12Q1/6809 ,  C12Q2565/501

    Abstract: The invention provides methods of monitoring expression of a plurality of genes in a cell or small population of cells. Preferred methods entail contacting an array of probes with a population of nucleic acids derived from a population of fewer than 1000 cells then determining the relative hybridization of the probes to the population of nucleic acid as a measure of the relative representation of genes from the cells. The invention further provides methods of classifying cells. These preferred methods entail determining an expression profile of each of a plurality of cells then classifying the cells in clusters determined by similarity of expression profile. The invention further provides methods of monitoring differentiation of a cell lineage. These preferred methods entail determining an expression profile of each of a plurality of cells at different differentiation stages within the lineage. These cells can then be classified into clusters determined by similarity of expression profile. The clusters can then be ordered by similarity of expression profile. A time course of expression levels for each of the plurality of genes at different stages of differentiation in the cell lineage can then be determined.

    Abstract translation: 本发明提供监测细胞或细胞群体中多种基因的表达的方法。 优选的方法需要将探针阵列与源自少于1000个细胞的群体的核酸群接触，然后确定探针与核酸群体的相对杂交，作为来自细胞的基因相对表达的量度。 本发明还提供了对细胞进行分类的方法。 这些优选方法需要确定多个细胞中的每一个的细胞的表达谱，然后通过表达谱的相似性确定的簇进行细胞分类。 本发明还提供监测细胞谱系分化的方法。 这些优选的方法需要确定谱系内不同分化阶段的多个细胞中的每一个的表达谱。 然后可以将这些细胞分类为通过表达谱的相似性确定的簇。 然后可以通过表达谱的相似性对簇进行排序。 然后可以确定细胞系中不同分化阶段的多个基因中每个基因的表达水平的时间过程。

Patent Agency Ranking