-
公开(公告)号：US09983155B2
公开(公告)日：2018-05-29
申请号：US13840994
申请日：2013-03-15
Applicant: Canon U.S. Life Sciences, Inc.
Inventor： Ivor T. Knight , Deborah John Boles , Aaron Rulison , Wesley B. Dong , Andrew Fabans , Allen Boronkay , Edward Donlon , Robert Moti , Michael Slater , Steven A. Sundberg , Michael R. Knapp
CPC classification number: G01N25/04 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/52 , B01L7/525 , B01L2200/10 , B01L2300/0627 , B01L2300/0816 , B01L2300/14 , B01L2300/18 , B01L2300/1833 , B01L2400/0409 , B01L2400/0415 , B01L2400/0487 , C12Q1/68 , C12Q1/6844 , C12Q1/686 , C12Q1/6862 , G01N21/33 , G01N21/6428 , Y10T436/143333 , C12Q2565/629 , C12Q2565/101 , C12Q2527/107 , C12Q2527/101
Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.
-
公开(公告)号：US09939336B2
公开(公告)日：2018-04-10
申请号：US14451214
申请日：2014-08-04
Applicant: Canon U.S. Life Sciences, Inc.
Inventor： Johnathan S. Coursey , Kenton C. Hasson , Gregory H. Owen
Abstract: The invention relates to systems and methods for calibrating and using resistance temperature detectors. In one embodiment, the system includes a calibration circuit comprising a resistance temperature detector in a bridge circuit with at least one potentiometer, and a programmable gain amplifier coupled to the bridge circuit. Embodiments of the invention further comprise methods for calibrating the bridge circuit and the programmable gain amplifier for use with the resistance temperature detector and methods for determining the self heating voltage of the bridge circuit.
-
公开(公告)号：US09823135B2
公开(公告)日：2017-11-21
申请号：US14981121
申请日：2015-12-28
Applicant: Canon U.S. Life Sciences, Inc.
Inventor： Gregory H. Owen , Gregory A. Dale , Kenton C. Hasson
CPC classification number: B01L7/525 , B01L3/5027 , B01L3/502715 , B01L7/52 , B01L2200/147 , B01L2300/0816 , B01L2300/1805 , B01L2300/1827 , B01L2300/1833 , C12Q1/686 , G01K7/16
Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one aspect, the present invention provides a method for controlling the temperature of a heater electrode associated with a microfluidic channel of a microfluidic device, wherein power applied to the heater electrode is regulated by varying the duty cycle of a pulse width modulation (PWM). In another aspect, the present invention a controller configured to compute the temperature of the heater electrode during the power-on portion of the duty cycle and the during the power-off portion of the duty cycle and to adjust the duty cycle as necessary to achieve a desired temperature in the heater electrode.
-
公开(公告)号：US09804068B2
公开(公告)日：2017-10-31
申请号：US14042043
申请日：2013-09-30
Applicant: Canon U.S. Life Sciences, Inc.
Inventor： Jeffrey M Burke , Ian M White
CPC classification number: G01N1/34 , B01D21/0087 , B01D21/2444 , B01D21/265 , B01L3/502753 , B01L3/502776 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , G01N1/4077
Abstract: A spiral inertial filtration device is capable of high-throughput (1 mL/min), high-purity particle separation while concentrating recovered target particles by more than an order of magnitude. Large fractions of sample fluid are removed from a microchannel without disruption of concentrated particle streams by taking advantage of particle focusing in inertial spiral microfluidics, which is achieved by balancing inertial lift forces and Dean drag forces. To enable the calculation of channel geometries in the device for specific concentration factors, an equivalent circuit model was developed and experimentally validated. Large particle concentration factors were achieved by maintaining either average fluid velocity or Dean number throughout the entire length of the channel during the incremental removal of sample fluid. Also provided is the ability to simultaneously separate more than one particle from the same sample.
-
公开(公告)号：USD799713S1
公开(公告)日：2017-10-10
申请号：US29509087
申请日：2014-11-13
Applicant: Canon U.S. Life Sciences, Inc.
Designer： Brian Murphy , Alex Flamm , Eric Schneider , Chris Felice , Kazuhiko Hasegawa , Ronald Kurz , Scott Sundberg , Ralph McCann , Jason Clemons
-
Patent Agency Ranking
公开(公告)号：US09777318B2
公开(公告)日：2017-10-03
申请号：US14697036
申请日：2015-04-27
Applicant: Canon U.S. Life Sciences, Inc.
Inventor： Kenton C. Hasson
IPC: C12Q1/68
CPC classification number: C12Q1/686
Abstract: A system and method for amplifying and detecting nucleic acids are disclosed. In one embodiment, the system includes: a microfluidic device comprising a channel for receiving a sample of solution containing real-time PCR reagents; a temperature control system configured to cycle the temperature of the sample; an excitation source for illuminating the sample; a fiber optic probe comprising (i) an optical fiber having a distal end and a proximal end and (ii) a probe head connected to the distal end of the optical fiber and positioned between the distal end of the optical fiber and the channel; and a detector configured to detect emissions exiting the proximal end of the optical fiber.
-
公开(公告)号：US20170176318A1
公开(公告)日：2017-06-22
申请号：US15388582
申请日：2016-12-22
Inventor： Giuliano Scarcelli , Jitao Zhang , Antonio Fiore , Hanyoup Kim
IPC: G01N15/14
CPC classification number: G01N15/1434 , G01N15/1459 , G01N15/147 , G01N2015/0065 , G01N2015/1006 , G01N2015/1495 , G01N2021/638
Abstract: The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.
-
公开(公告)号：US20170108384A1
公开(公告)日：2017-04-20
申请号：US15389899
申请日：2016-12-23
Applicant: Canon U.S. Life Sciences, Inc.
Inventor： Kenton C. Hasson , Johnathan S. Coursey , Gregory H. Owen , Gregory A. Dale
CPC classification number: B01L7/525 , B01L3/5027 , B01L3/502715 , B01L7/52 , B01L2200/147 , B01L2300/0816 , B01L2300/1805 , B01L2300/1827 , B01L2300/1833 , C12Q1/686 , G01K7/16
Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature each heater electrode by determining the resistance of each heater electrode.
-
公开(公告)号：US20170009279A1
公开(公告)日：2017-01-12
申请号：US15269362
申请日：2016-09-19
Applicant: Canon U.S. Life Sciences, Inc.
Inventor： Ling Xu , Renee Howell
IPC: C12Q1/68
CPC classification number: C12Q1/6827 , C12Q1/6883 , C12Q2600/156 , C12Q2525/204 , C12Q2527/107 , C12Q2531/107
Abstract: The present invention relates to methods, kits, probes, and systems for distinguishing between nucleotide variants that are close in proximity on a gene. The methods, kits, probes, and systems can include the use of a small amplicon assay in combination with two unlabeled probes in a high resolution thermal melting analysis of a biological sample containing a locus of interest in order to discern between disease-causing and benign variants that are close in proximity on a gene within the biological sample. The present invention also relates to method of detecting a disease in a patient based on the patient's genotype by determining whether the patient has a disease-causing variant at a locus of interest. The signature melt curves produced by the unlabeled probe tests can be analyzed using HRMA software to distinguish between disease-causing and benign variants that are close in proximity on a gene within the biological sample.
Abstract translation: 本发明涉及用于区分在基因附近接近的核苷酸变体的方法，试剂盒，探针和系统。 方法，试剂盒，探针和系统可以包括在含有感兴趣的基因座的生物样品的高分辨率热熔解分析中与两个未标记的探针组合使用小的扩增子测定，以鉴别致病和良性 在生物样品中的基因附近接近的变体。 本发明还涉及通过确定患者是否在感兴趣的场所具有致病变体，基于患者的基因型来检测患者的疾病的方法。 可以使用HRMA软件分析由未标记的探针测试产生的标记融合曲线，以区分生物样品中基因附近接近的致病因子和良性变体。
-
公开(公告)号：US20160342736A1
公开(公告)日：2016-11-24
申请号：US15076217
申请日：2016-03-21
Applicant: Canon U.S. Life Sciences, Inc.
Inventor： Deborah BOLES , Kenton C. HASSON , Sami KANDERIAN
IPC: G06F19/24
CPC classification number: G16B40/00 , B01L7/52 , C12Q1/6816 , G16B20/00 , G16B25/00 , C12Q2527/107 , C12Q2537/165 , C12Q2539/101
Abstract: The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. Methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. Methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.
Abstract translation: 本发明涉及用于分析核酸的解离行为和核酸鉴定的方法和系统。 公开了用于鉴定样品中的核酸的方法和系统，所述样品包括未知核酸并用于检测样品中核酸中的单核苷酸多态性。 公开了用于通过将包括在多个独立样品特性点上的样品的可量化物理变化的测量的变性数据拟合到确定一个或多个独立样品特性点的函数来鉴定包含至少一种未知核酸的生物样品中的核酸的方法和系统 并且获得估计的物理变化功能，以及通过将至少一种未知核酸的固有物理值与已知核酸的固有物理值进行比较来鉴定生物样品中的核酸。
-
-
-
-
-
-
-
-
-
Search Results
125
records
(took 0.029 seconds)
