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公开(公告)号：US20210232569A1
公开(公告)日：2021-07-29
申请号：US16751983
申请日：2020-01-24
Inventor： Aaron Kennington
IPC: G06F16/23 , G06F16/2453 , G01N15/14
Abstract: The multi-parameter data produced via flow cytometry and other biological analyses techniques can generate enormous amounts of data, which can take extensive time and/or computational resources to complete. Embodiments provided herein allow for adaptive sub-sampling of such data prior to analysis, allowing for such analyses to be performed while satisfying certain performance criteria. Such performance criteria may include, for example, keeping the latency of the analysis below a specified duration. This can allow analysis of data to be performed in real time as the data is generated, e.g., as flow cytometry data is generated by a cell counter or other flow cytometry instrument. This can also permit for data analyses to be iteratively developed or improved in less time by adaptively sub-sampling the data prior to re-analysis, so that the total time between iterations is reduced.
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公开(公告)号：US20210089750A1
公开(公告)日：2021-03-25
申请号：US16950368
申请日：2020-11-17
Inventor： Timothy Jackson , Nevine Holtz , Christoffer Edlund , Rickard Sjögren
Abstract: A method of analyzing images of a biological specimen using a computational model is described, the method including processing a cell image of the biological specimen and a phase contrast image of the biological specimen using the computational model to generate an output data. The cell image is a composite of a first brightfield image of the biological specimen at a first focal plane and a second brightfield image of the biological specimen at a second focal plane. The method also includes performing a comparison of the output data and a reference data and refining the computational model based on the comparison of the output data and the reference data. The method also includes thereafter processing additional image pairs according to the computational model to further refine the computational model based on comparisons of additional output data generated by the computational model to additional reference data.
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Patent Agency Ranking
公开(公告)号：US10885631B2
公开(公告)日：2021-01-05
申请号：US16265910
申请日：2019-02-01
Applicant: Essen Instruments, Inc.
Inventor： Timothy R. Jackson , Nevine Holtz
Abstract: The disclosure provides example methods that include a processor: (a) generating at least one phase contrast image of a biological specimen comprising one or more cells centered around a focal plane for the biological specimen; (b) generating a confluence mask in the form of a binary image based on the at least one phase contrast image; (c) receiving a first brightfield image of the biological specimen at a defocusing distance above the focal plane and a second brightfield image of the biological specimen at the defocusing distance below the focal plane; (d) generating a cell image of the biological specimen based on the first and second brightfield image; (e) generating a seed mask based on the cell image and the phase contrast image; and (f) generating an image of the biological specimen showing a cell-by-cell segmentation mask based on the seed mask and the confluence mask.
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34.发明授权公开(公告)号：US10739246B2
公开(公告)日：2020-08-11
申请号：US16138407
申请日：2018-09-21
Applicant: Essen Instruments, Inc.
Abstract: A method for evaluating a biological material for unassociated virus-size particles having an adenovirus epitope uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-size particle and fluorescent antibody stain.
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公开(公告)号：US10585030B2
公开(公告)日：2020-03-10
申请号：US15713314
申请日：2017-09-22
Applicant: Essen Instruments, Inc.
Abstract: A method for evaluating a biological material for unassociated virus-like particles virus size having a particular epitope uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-like particle and fluorescent antibody stain.
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公开(公告)号：US10545084B2
公开(公告)日：2020-01-28
申请号：US16216535
申请日：2018-12-11
Applicant: Essen Instruments, Inc.
Abstract: A method for evaluating a biological material for unassociated virus-size particles of exosomes having a particular epitope uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the exosomes and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such an exosome and fluorescent antibody stain.
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37.发明申请公开(公告)号：US20140127744A1
公开(公告)日：2014-05-08
申请号：US14154766
申请日：2014-01-14
Applicant: Essen Instruments, Inc.
Inventor： Kirk Schroeder , Bradley D. Neagle
IPC: G01N33/50
CPC classification number: C12Q1/02 , B01L3/50255 , B01L3/5085 , B01L2200/0647 , B01L2200/142 , B01L2300/0654 , B01L2300/0681 , B01L2300/0829 , B01L2300/163 , G01N21/6452 , G01N21/6458 , G01N33/5008 , G01N33/5029 , G01N33/54366 , G01N33/58
Abstract: Apparatus and methods to improve the Boyden chamber used in cellular biological measurements, allowing quantitative optical microscopy of biological cells in situ without using fluorescent probes or optical staining. In the preferred embodiment, a thin porous membrane separating top and bottom reservoirs includes an array of precisely positioned micropores pores manufactured using a laser-based photo-machining (ablation) process. The membrane may be composed of polyethylene terephthalate (PET), polycarbonate, polyimide, polyether ether ketone (PEEK) or other appropriate material. The pores formed in the membrane may have diameters in the range of 1 to 15 microns and spaced apart at a distance ranging from 10 to 200 microns. A plurality of upper and lower reservoirs may be provided to form a multi-well plate. The invention finds application in a wide range of potential biological applications where Boyden chamber geometries are currently used including co-culture studies, tissue remodeling studies, cell polarity determinations, endocrine signaling, cell transport, cell permeability, cell invasion and chemotaxis assays.
Abstract translation: 用于改善细胞生物学测量中使用的Boyden室的装置和方法，允许原位生物细胞的定量光学显微镜检查，而不使用荧光探针或光学染色。 在优选实施例中，分离顶部和底部储存器的薄多孔膜包括使用基于激光的光加工（消融）工艺制造的精确定位的微孔的阵列。 膜可以由聚对苯二甲酸乙二醇酯（PET），聚碳酸酯，聚酰亚胺，聚醚醚酮（PEEK）或其它合适的材料组成。 形成在膜中的孔可以具有在1至15微米范围内的直径并且间隔开10至200微米的距离。 可以设置多个上部和下部储存器以形成多孔板。 本发明应用于广泛的潜在生物应用，其中目前使用Boyden室几何形状，包括共培养研究，组织重塑研究，细胞极性测定，内分泌信号，细胞转运，细胞通透性，细胞侵袭和趋化性测定。
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