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公开(公告)号：US06420140B1
公开(公告)日：2002-07-16
申请号：US09769070
申请日：2001-01-24
IPC分类号： C12P2104
CPC分类号： C07K14/00 , C07K16/00 , C07K16/244 , C07K2317/21 , C12N5/16 , C12N5/166 , C12N15/62 , C12N2510/02
摘要： The present invention features a method of producing a multimeric protein from a hybrid cell formed from the fusion of two or more cells, each of which cell is engineered to express one component of the multimeric protein, as well as a method for screening for successful fusion of the cells to produce a desired hybrid cell. The methods of the invention are widely applicable to the production of proteins having two or more components.
摘要翻译： 本发明的特征在于从由两个或多个细胞的融合形成的杂交细胞产生多聚体蛋白质的方法，每个细胞被工程化以表达多聚体蛋白质的一个组分，以及筛选成功融合的方法 的细胞以产生所需的杂交细胞。 本发明的方法可广泛用于生产具有两种或多种组分的蛋白质。
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32.发明授权Method for increasing hematopoietic progenitor cells by stem cell factor polypeptides 失效通过干细胞因子多肽增加造血祖细胞的方法公开(公告)号：US06248319B1
公开(公告)日：2001-06-19
申请号：US08449653
申请日：1995-05-24
IPC分类号： A61K3819
CPC分类号： A61K35/28 , A61K38/00 , A61K38/1816 , A61K38/193 , A61K38/202 , A61K38/204 , C07K14/475 , C07K16/22 , Y02A50/411 , A61K2300/00
摘要： Novel stem cell factors, oligonucleotides encoding the same, and methods of production, are disclosed. Pharmaceutical compositions and methods of treating disorders involving blood cells are also disclosed.
摘要翻译： 公开了新型干细胞因子，编码它们的寡核苷酸和生产方法。 还公开了治疗涉及血细胞的疾病的药物组合物和方法。
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公开(公告)号：US06207418B1
公开(公告)日：2001-03-27
申请号：US09342301
申请日：1999-06-29
IPC分类号： C12P2104
CPC分类号： C07K16/244 , C07K14/00 , C07K16/00 , C07K2317/21 , C12N5/16 , C12N5/166 , C12N2510/02
摘要： The present invention features a method of producing a multimeric protein from a hybrid cell formed from the fusion of two or more cells, each of which cell is engineered to express one component of the multimeric protein, as well as a method for screening for successful fusion of the cells to produce a desired hybrid cell. The methods of the invention are widely applicable to the production of proteins having two or more components.
摘要翻译： 本发明的特征在于从由两个或多个细胞的融合形成的杂交细胞产生多聚体蛋白质的方法，每个细胞被工程化以表达多聚体蛋白质的一个组分，以及筛选成功融合的方法 的细胞以产生所需的杂交细胞。 本发明的方法可广泛用于生产具有两种或多种组分的蛋白质。
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34.发明授权Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells 失效生产高滴度病毒和高效逆转录病毒介导的哺乳动物细胞转导的方法
公开(公告)号：US5858740A
公开(公告)日：1999-01-12
申请号：US438582
申请日：1995-05-10
申请人： Mitchell H. Finer , Margo R. Roberts , Thomas J. Dull , Krisztina M. Zsebo , Lu Qin , Deborah A. Farson
发明人： Mitchell H. Finer , Margo R. Roberts , Thomas J. Dull , Krisztina M. Zsebo , Lu Qin , Deborah A. Farson
IPC分类号： C12N15/09 , A61K31/70 , A61K35/76 , A61K48/00 , C07H21/04 , C07K14/15 , C07K14/72 , C07K14/725 , C07K14/73 , C07K16/10 , C12N5/10 , C12N7/00 , C12N7/04 , C12N15/867 , C12N15/87 , C12R1/92 , C12N15/10 , C12N15/86
CPC分类号： C12N7/00 , C07K14/005 , C07K14/7051 , C07K14/70514 , C07K14/721 , C07K16/10 , C12N15/86 , C12N15/87 , A61K48/00 , C07K2319/00 , C12N2740/13022 , C12N2740/13043 , C12N2740/13052 , C12N2740/13062
摘要： The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.
摘要翻译： 本发明提供了一种新的逆转录病毒包装系统，其中通过在人细胞中转染从高表达质粒产生逆转录病毒包装构建体和可包装载体转录物。 在感染的细胞中产生高滴度的重组逆转录病毒。 本发明的方法包括使用新型逆转录病毒构建体通过以高效率的共培养将外源基因转导到包含T细胞和人造血干细胞的原代人细胞中。 本发明可用于快速生产高病毒上清液，并且可以通过常规方法转导难以转导的高效细胞。
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