公开(公告)号：US07090852B2

公开(公告)日：2006-08-15

申请号：US10267322

申请日：2002-10-09

Applicant: Michael C. Hevey ,  Diane L. Negley ,  Peter Pushko ,  Jonathan F. Smith ,  Alan L. Schmaljohn

Inventor： Michael C. Hevey ,  Diane L. Negley ,  Peter Pushko ,  Jonathan F. Smith ,  Alan L. Schmaljohn

IPC: A61K39/12

CPC classification number: C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K16/10 ,  C12N7/00 ,  C12N15/86 ,  C12N2760/14022 ,  C12N2760/14222 ,  C12N2770/36021 ,  C12N2770/36122 ,  C12N2770/36123 ,  C12N2770/36143 ,  C12N2770/36162

Abstract: Using the MBGV GP, NP, and virion proteins, a method and composition for use in inducing an immune response which is protective against infection with MBGV in nonhuman primates is described.

Abstract translation: 描述了使用MBGV GP，NP和病毒粒子蛋白，用于诱导免疫应答的方法和组合物，其在非人灵长类动物中防止MBGV感染。

公开(公告)号：US06770479B1

公开(公告)日：2004-08-03

申请号：US09350729

申请日：1999-07-09

Applicant: John S. Lee ,  Peter Pushko ,  Michael D. Parker ,  Jonathan F. Smith ,  Susan L. Welkos

Inventor： John S. Lee ,  Peter Pushko ,  Michael D. Parker ,  Jonathan F. Smith ,  Susan L. Welkos

IPC: C12N1586

CPC classification number: C07K14/32 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/53 ,  C07K14/31 ,  C12N2799/021

Abstract: Using the nontoxic PA protein from B. anthracis, a method and composition for use in inducing an immune response which is protective against anthrax in subjects is described.

Abstract translation: 使用来自炭疽杆菌的无毒PA蛋白，描述了用于诱导受试者中对炭疽病有保护作用的免疫应答的方法和组合物。

公开(公告)号：US06495143B2

公开(公告)日：2002-12-17

申请号：US09350756

申请日：1999-07-09

Applicant: John S. Lee ,  Peter Pushko ,  Jonathan F. Smith ,  Michael Parker ,  Mark T. Dertzbaugh ,  Leonard Smith

Inventor： John S. Lee ,  Peter Pushko ,  Jonathan F. Smith ,  Michael Parker ,  Mark T. Dertzbaugh ,  Leonard Smith

IPC: C12N1586

CPC classification number: C07K14/32 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/53 ,  C07K14/31 ,  C07K14/33 ,  C12N2799/021

Abstract: Using the nontoxic heavy chain fragment from botulinum neurotoxins A-G, compositions and methods of use in inducing an immune response which is protective against intoxication with botulinum in subjects is described.

Abstract translation: 使用来自肉毒杆菌神经毒素A-G的无毒重链片段，描述了用于诱导免疫应答的组合物和方法，其在受试者中与肉毒杆菌中毒。

公开(公告)号：US06296854B1

公开(公告)日：2001-10-02

申请号：US09454721

申请日：1999-12-07

Applicant: Peter Pushko ,  Michael D. Parker ,  Jonathan F. Smith ,  Bruce J. Crise

Inventor： Peter Pushko ,  Michael D. Parker ,  Jonathan F. Smith ,  Bruce J. Crise

IPC: A61K3912

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61K39/193 ,  A61K2039/5254 ,  C12N7/00 ,  C12N2770/36122 ,  C12N2770/36134 ,  C12N2770/36161 ,  Y10S424/82

Abstract: A live attenuated Venezuelan equine encephalitis virus (VEE) is described which comprises a viral gene rearrangement. This rearranged attenuated virus is useful as vaccine for protection against infection with VEE. Methods of preparing the virus and methods of using the virus are described.

Abstract translation: 描述了减毒的委内瑞兰马脑炎病毒（VEE），其包含病毒基因重排。 这种重排的减毒病毒可用作防止VEE感染的疫苗。 描述了制备病毒的方法和使用该病毒的方法。

公开(公告)号：US09101572B2

公开(公告)日：2015-08-11

申请号：US13519948

申请日：2011-01-03

Applicant: Peter Pushko ,  Irina Tretyakova ,  Igor Lukashevich

Inventor： Peter Pushko ,  Irina Tretyakova ,  Igor Lukashevich

IPC: A61K39/12 ,  C07K14/005 ,  A61K39/00

CPC classification number: A61K39/12 ,  A61K49/0004 ,  A61K2039/5254 ,  A61K2039/53 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36134 ,  C12N2770/36151 ,  Y02A50/383

Abstract: Described herein are i-DNA™ vectors and vaccines and methods for using the same. The i-DNA™ generates live attenuated vaccines in eukaryotic cells in vitro or in vivo for pathogenic RNA viruses, particularly chikungunya virus (CHIKV). When iDNA is injected into the vaccine recipient, RNA of live attenuated virus is generated by in vivo transcription in the recipient's tissues. This initiates production of progeny attenuated viruses in the tissues of the vaccine recipient, as well as elicitation of an effective immune response protecting against wild-type, non-attenuated virus.

Abstract translation: 本文描述的是i-DNA TM载体和疫苗及其使用方法。 i-DNA TM在真核细胞中在体外或体内产生致病性RNA病毒特别是基孔肯雅病毒（CHIKV）的活减毒疫苗。 当将iDNA注射到疫苗接受者中时，活体减毒病毒的RNA通过受体组织中的体内转录产生。 这引发疫苗接种者组织中后代减毒病毒的产生，以及引发保护免受野生型非减毒病毒的有效免疫应答。

公开(公告)号：US08715692B2

公开(公告)日：2014-05-06

申请号：US12633995

申请日：2009-12-09

Applicant: Peter Pushko ,  Yingyun Wu ,  Michael Massare ,  Ye Liu ,  Gale Smith ,  Bin Zhou

Inventor： Peter Pushko ,  Yingyun Wu ,  Michael Massare ,  Ye Liu ,  Gale Smith ,  Bin Zhou

IPC: A61K39/155 ,  C07K14/135 ,  C12N15/40 ,  C12N5/10

CPC classification number: A61K39/155 ,  A61K9/0019 ,  A61K9/1075 ,  A61K39/00 ,  A61K39/12 ,  A61K39/39 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/55 ,  A61K2039/55505 ,  A61K2039/57 ,  A61K2039/575 ,  C07K14/005 ,  C07K2319/00 ,  C12N7/00 ,  C12N2760/18522 ,  C12N2760/18523 ,  C12N2760/18534 ,  C12N2760/18562 ,  C12N2760/18571

Abstract: The present invention is generally related to modified or mutated respiratory syncytial virus fusion (F) proteins and methods for making and using them, including immunogenic compositions such as vaccines for the treatment and/or prevention of RSV infection.

Abstract translation: 本发明通常涉及修饰或突变的呼吸道合胞病毒融合（F）蛋白及其制备及使用方法，包括用于治疗和/或预防RSV感染的疫苗等免疫原性组合物。

公开(公告)号：US08697088B2

公开(公告)日：2014-04-15

申请号：US12127625

申请日：2008-05-27

Applicant: Gale Smith ,  Peter Pushko ,  Kutub Mahmood ,  Bin Zhao

Inventor： Gale Smith ,  Peter Pushko ,  Kutub Mahmood ,  Bin Zhao

IPC: A61K39/145 ,  C12N15/44

CPC classification number: A61K39/145 ,  A61K2039/5258 ,  C07K2319/00 ,  C12N7/00 ,  C12N2710/16723 ,  C12N2760/16123

Abstract: The present invention discloses novel influenza virus-like particles (VLPs) that contain chimeric proteins or influenza membrane proteins. The chimeric proteins are derived from fragments of influenza membrane proteins fused to heterologous proteins. The invention includes antigenic formulations and vaccines comprising VLPs of the invention as well as methods of making and administering VLPs to vertebrates, including methods of inducing immunity to infections, such as influenza.

Abstract translation: 本发明公开了含有嵌合蛋白或流感病毒膜蛋白的新型流感病毒样颗粒（VLPs）。 嵌合蛋白质衍生自与异源蛋白质融合的流感病毒膜蛋白片段。 本发明包括抗原制剂和包含本发明的VLP的疫苗以及向脊椎动物制备和施用VLP的方法，包括诱导感染免疫的方法，例如流感。

公开(公告)号：US08551756B2

公开(公告)日：2013-10-08

申请号：US12689826

申请日：2010-01-19

Applicant: Gale Smith ,  Peter Pushko

Inventor： Gale Smith ,  Peter Pushko

IPC: C12N7/00 ,  C12N7/04

CPC classification number: A61K39/145 ,  A61K39/12 ,  A61K2039/5258 ,  C07H21/04 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14143 ,  C12N2760/16011 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16222 ,  C12N2760/16223 ,  C12N2760/16234 ,  C12N2760/18522 ,  C12N2760/18523 ,  C12N2770/20022 ,  C12N2770/20023 ,  C12N2770/20034 ,  Y02A50/386 ,  Y02A50/388

Abstract: This invention discloses a method of increasing production of virus-like particles comprising expressing an avian influenza matrix protein. The invention also comprises methods of making and using said VLPs.

Abstract translation: 本发明公开了一种增加包含表达禽流感基因蛋白质的病毒样颗粒的生产的方法。 本发明还包括制备和使用所述VLP的方法。

公开(公告)号：US20100184192A1

公开(公告)日：2010-07-22

申请号：US12689826

申请日：2010-01-19

Applicant: Gale Smith ,  Peter Pushko

Inventor： Gale Smith ,  Peter Pushko

IPC: C12N7/00

CPC classification number: A61K39/145 ,  A61K39/12 ,  A61K2039/5258 ,  C07H21/04 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14143 ,  C12N2760/16011 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16222 ,  C12N2760/16223 ,  C12N2760/16234 ,  C12N2760/18522 ,  C12N2760/18523 ,  C12N2770/20022 ,  C12N2770/20023 ,  C12N2770/20034 ,  Y02A50/386 ,  Y02A50/388

Abstract: This invention discloses a method of increasing production of virus-like particles comprising expressing an avian influenza matrix protein. The invention also comprises methods of making and using said VLPs

Abstract translation: 本发明公开了一种增加包含表达禽流感基因蛋白质的病毒样颗粒的生产的方法。 本发明还包括制备和使用所述VLP的方法

公开(公告)号：US20100035813A1

公开(公告)日：2010-02-11

申请号：US12509033

申请日：2009-07-24

Applicant: Gale SMITH ,  Peter Pushko ,  Vittoria Cioce

Inventor： Gale SMITH ,  Peter Pushko ,  Vittoria Cioce

IPC: A61K38/16 ,  C07K14/47 ,  C07K7/08 ,  C12N15/11 ,  C12N15/00 ,  C12N5/10 ,  C12N1/21 ,  C12P21/02

CPC classification number: C07K14/70564 ,  A61K38/00

Abstract: The inventive features include recombinant mammalian E-selectin peptides, nucleic acids encoding said peptides, vectors and cells having these nucleic acids, and methods of making the peptides. Further inventive features include methods of treating diseases and conditions associated with inflammation using recombinant mammalian E-selectin peptides to induce mucosal tolerance to E-selectin.

Abstract translation: 本发明的特征包括重组哺乳动物E-选择蛋白肽，编码所述肽的核酸，具有这些核酸的载体和细胞，以及制备肽的方法。 其它发明特征包括使用重组哺乳动物E-选择蛋白肽治疗与炎症相关的疾病和病症以诱导对E-选择素的粘膜耐受的方法。