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公开(公告)号：US10941380B2
公开(公告)日：2021-03-09
申请号：US15014762
申请日：2016-02-03
发明人： William Hudson Hofmeister , Lucas Hofmeister , Alexander Yuryevich Terekhov , Jose Lino Vasconcelos da Costa
IPC分类号： C12N5/00 , B29C67/20 , B29K67/00 , B29K105/00
摘要： Embodiments of the presently disclosed subject matter provide biomimetic cell culture substrates comprising highly tunable patterned polymer nanofiber matrices capable of modulating expression of critical self-renewal factors and markers of cell-cell interaction to maintain stemness of human mesenchymal stem cells in vitro. Embodiments of the presently-disclosed subject matter also provide scalable, highly repeatable methods of making biomimetic cell culture substrates by hot pressing thermoplastic polymer films into femtosecond laser-ablated nanopore molds to form patterned polymer nanofiber matrices on flat thermoplastic substrates.
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公开(公告)号：US10758902B2
公开(公告)日：2020-09-01
申请号：US15585424
申请日：2017-05-03
IPC分类号： B01D69/12 , C08J5/18 , B01L3/00 , B29D99/00 , G01N33/52 , B05D1/00 , B29C37/00 , B29C41/02 , B29C41/38 , B32B3/26 , B32B27/30 , G01N33/50 , B32B37/24 , B32B37/12 , B32B38/10 , B29K67/00 , B29K629/00 , B29L31/00 , B32B38/00
摘要： A semipermeable ultrathin polymer membrane comprises a substantially optically transparent polymer film having a surface area to thickness ratio of at least 1,000,000:1, and an array of precisely spatially ordered pores of a user-selected diameter defined therethrough. Such membranes can be fabricated by providing a mold having a patterned array of nanoholes femtosecond laser ablated in a surface thereof; applying a first polymer solution onto the mold surface so that the first polymer solution infiltrates the nanoholes; allowing the first polymer solution to dry and form a replica of the mold having a plurality of freestanding nanoneedles extending from a surface of the replica; removing the replica from the mold; coating the replica surface with a second polymer solution; drying the second polymer solution to form a porous polymer film; and dissolving the replica in a solvent to release the film from the replica as a semipermeable ultrathin polymer membrane.
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公开(公告)号：US20190076775A1
公开(公告)日：2019-03-14
申请号：US16185231
申请日：2018-11-09
摘要： A tunable nanofiber filter device can include a filter housing defining an interior space, the housing having defined therein and inlet and an outlet, each in fluid communication with the interior space, and a plurality of filter laminas disposed within the interior space, each filter lamina including an upper surface, a lower surface, and an aperture defined therethrough. The plurality of filter laminas can be arranged in a stack wherein the opposing surfaces of adjacent filter laminas define a portion of an interlaminar flow space extending between the opposing surfaces. The flow space can be in fluid communication with the apertures of corresponding adjacent filter laminas to form a continuous flow passage extending through the lamina stack from the inlet to the outlet. An array nanofibers can extend into the flow passage from a portion of each filter lamina such that a fluid flowed through the flow passage flows across a portion of said array.
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公开(公告)号：US10159926B2
公开(公告)日：2018-12-25
申请号：US15260677
申请日：2016-09-09
摘要： A tunable nanofiber filter device can include a filter housing defining an interior space, the housing having defined therein and inlet and an outlet, each in fluid communication with the interior space, and a plurality of filter laminas disposed within the interior space, each filter lamina including an upper surface, a lower surface, and an aperture defined therethrough. The plurality of filter laminas can be arranged in a stack wherein the opposing surfaces of adjacent filter laminas define a portion of an interlaminar flow space extending between the opposing surfaces. The flow space can be in fluid communication with the apertures of corresponding adjacent filter laminas to form a continuous flow passage extending through the lamina stack from the inlet to the outlet. An array nanofibers can extend into the flow passage from a portion of each filter lamina such that a fluid flowed through the flow passage flows across a portion of said array.
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公开(公告)号：US20150093550A1
公开(公告)日：2015-04-02
申请号：US14560833
申请日：2014-12-04
发明人： William Hudson Hofmeister , Alexander Yuryevich Terekhov , Jose Lino Vasconcelos de Costa , Kathleen Stacia Lansford , Deepak Rajput , Lloyd M. Davis
IPC分类号： B29C41/00 , B29C41/42 , B29C33/38 , B29C41/38 , C08G63/08 , C08B5/02 , C08G77/04 , C08F120/18 , C08F120/40 , C08G63/664 , C08B3/06 , B29C41/02 , C08F110/02
CPC分类号： B29C41/003 , B29C33/3842 , B29C41/02 , B29C41/38 , B29C41/42 , B29K2001/12 , B29K2001/18 , B29K2023/06 , B29K2031/04 , B29K2033/12 , B29K2067/04 , B29K2071/00 , B29K2083/00 , B29K2901/10 , B29K2901/12 , B29K2907/045 , B29K2909/00 , B29K2909/08 , B29K2909/14 , B29K2995/0026 , B29K2995/0094 , B29L2031/7562 , B82Y10/00 , B82Y40/00 , C08B3/06 , C08B5/02 , C08F110/02 , C08F120/18 , C08F120/40 , C08G63/08 , C08G63/664 , C08G77/04 , G03F7/0002 , Y02P20/582 , Y10T428/24355 , Y10T428/24802
摘要： Solution casting a nanostructure. Preparing a template by ablating nanoholes in a substrate using single-femtosecond laser machining. Replicating the nanoholes by applying a solution of a polymer and a solvent into the template. After the solvent has substantially dissipated, removing the replica from the substrate.
摘要翻译： 溶液浇铸纳米结构。 通过使用单飞秒激光加工在衬底中烧蚀纳米孔来制备模板。 通过将聚合物和溶剂的溶液施加到模板中来复制纳孔。 在溶剂基本消散之后，从基材上除去复制品。
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