利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.015 秒)

    SEMIPERMEABLE ULTRATHIN POLYMER MEMBRANES
    1.
    发明申请
    SEMIPERMEABLE ULTRATHIN POLYMER MEMBRANES 审中-公开

    公开(公告)号：US20170320057A1

    公开(公告)日：2017-11-09

    申请号：US15585424

    申请日：2017-05-03

    申请人： Ultra Small Fibers, LLC

    发明人： William Hudson Hofmeister ,  Alexander Yuryevich Terekhov ,  Jose Lino Vasconcelos da Costa

    IPC分类号： B01L3/00 ,  G01N33/50 ,  B32B3/26 ,  B32B38/00 ,  B29C37/00 ,  B29C41/02 ,  B29C41/38 ,  B32B27/30 ,  C08J5/18 ,  B05D1/00 ,  B29L31/00 ,  B29K67/00

    摘要： A semipermeable ultrathin polymer membrane is a microfluidic device that comprises a substantially optically transparent polymer film having a surface area to thickness ratio of at least 1,000,000:1, and an array of precisely spatially ordered pores of a user-selected diameter defined therethrough. Such membranes can be fabricated by providing a mold having a patterned array of nanoholes femtosecond laser ablated in a surface thereof; applying a first polymer solution onto the mold surface so that the first polymer solution infiltrates the nanoholes; allowing the first polymer solution to dry and form a replica of the mold having a plurality of freestanding nanoneedles extending from a surface of the replica; removing the replica from the mold; coating the replica surface with a second polymer solution; drying the second polymer solution to form a porous polymer film; and dissolving the replica in a solvent to release the film from the replica as a semipermeable ultrathin polymer membrane. Also disclosed are multi-chambered microfluidic devices for studying cell biology in vitro that incorporate one or more such semipermeable ultrathin polymer membranes.

    Biomimetic Cell Culture Substrates
    6.
    发明申请
    Biomimetic Cell Culture Substrates 审中-公开
    生物模拟细胞培养基质

    公开(公告)号：US20160222345A1

    公开(公告)日：2016-08-04

    申请号：US15014762

    申请日：2016-02-03

    申请人： Ultra Small Fibers, LLC

    发明人： William Hudson Hofmeister ,  Lucas Hofmeister ,  Alexander Yuryevich Terekhov ,  Jose Lino Vasconcelos da Costa

    IPC分类号： C12N5/00 ,  B29C67/20

    CPC分类号： C12N5/0068 ,  B29C67/20 ,  B29K2067/04 ,  B29K2101/12 ,  B29K2105/256 ,  C12N2533/30 ,  C12N2535/10

    摘要： Embodiments of the presently disclosed subject matter provide biomimetic cell culture substrates comprising highly tunable patterned polymer nanofiber matrices capable of modulating expression of critical self-renewal factors and markers of cell-cell interaction to maintain stemness of human mesenchymal stem cells in vitro. Embodiments of the presently-disclosed subject matter also provide scalable, highly repeatable methods of making biomimetic cell culture substrates by hot pressing thermoplastic polymer films into femtosecond laser-ablated nanopore molds to form patterned polymer nanofiber matrices on flat thermoplastic substrates.

    摘要翻译： 目前公开的主题的实施方案提供了包含高度可调图案的聚合物纳米纤维基质的仿生细胞培养基质，其能够调节临界自我更新因子的表达和细胞 - 细胞相互作用的标记以维持体外人间质干细胞的干性。 目前公开的主题的实施方案还提供可扩展的，高度可重复的制造仿生细胞培养基质的方法，其通过将热塑性聚合物膜热压到飞秒激光烧蚀的纳米孔模具中以在平坦的热塑性基材上形成图案化的聚合物纳米纤维基质。