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    SITE-SPECIFIC INTEGRATION AND STACKING OF TRANSGENES IN SOYBEAN VIA DNA RECOMBINASE MEDIATED CASSETTE EXCHANGE
    33.
    发明申请
    SITE-SPECIFIC INTEGRATION AND STACKING OF TRANSGENES IN SOYBEAN VIA DNA RECOMBINASE MEDIATED CASSETTE EXCHANGE 有权
    通过DNA重组体介导的CASSETTE交换的大豆的特异性整合和转化大肠杆菌

    公开(公告)号：US20130014294A1

    公开(公告)日：2013-01-10

    申请号：US13614000

    申请日：2012-09-13

    申请人： Saverio Carl Falco ,  Zhongsen Li

    发明人： Saverio Carl Falco ,  Zhongsen Li

    IPC分类号： C12N15/82 ,  A01H5/10 ,  C12N5/10 ,  A01H5/00

    CPC分类号： C12N15/8213

    摘要： A targeting method is described that allows precise cassette replacement at a previously characterized genetic locus. A target DNA construct containing a pair of incompatible FRT sites flanking a target cassette was introduced into soybean by regular biolistic transformation. Transgenic events containing a single complete copy of the target site were then selected and retransformed with a donor DNA construct containing the identical pair of incompatible FRT sites flanking a donor cassette. Precise DNA cassette exchange happened between the target cassette and the donor cassette via recombinase mediated cassette exchange (RMCE) so that the donor cassette was introduced at the exact genomic site previously occupied by the target cassette. Through repeated RMCE using additional incompatible FRT sites, multiple groups of transgenes can be stacked at the same genomic locus.

    摘要翻译： 描述了靶向方法，其允许在先前表征的遗传基因座处进行精确的盒替换。 通过常规的生物弹性转化，将含有目标盒侧翼的一对不相容的FRT位点的靶DNA构建体引入大豆。 然后选择含有单个完整拷贝的靶位点的转基因事件，并用含有供体盒侧翼的相同对不对称FRT位点的供体DNA构建体重新转化。 通过重组酶介导的盒式交换（RMCE）在靶盒和供体盒之间发生精确的DNA盒交换，使得供体盒被导入到由靶盒预先占据的确切基因组位点。 通过反复RMCE使用额外的不相容的FRT位点，可以在相同的基因组座位堆叠多组转基因。

    Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange
    35.
    发明授权
    Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange 有权
    通过DNA重组酶介导的盒式交换，在大豆中转基因的位点特异性整合和堆积

    公开(公告)号：US08293533B2

    公开(公告)日：2012-10-23

    申请号：US12634775

    申请日：2009-12-10

    申请人： Saverio Carl Falco ,  Zhongsen Li

    发明人： Saverio Carl Falco ,  Zhongsen Li

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8213

    摘要： A targeting method is described that allows precise cassette replacement at a previously characterized genetic locus. A target DNA construct containing a pair of incompatible FRT sites flanking a target cassette was introduced into soybean by regular biolistic transformation. Transgenic events containing a single complete copy of the target site were then selected and retransformed with a donor DNA construct containing the identical pair of incompatible FRT sites flanking a donor cassette. Precise DNA cassette exchange happened between the target cassette and the donor cassette via recombinase mediated cassette exchange (RMCE) so that the donor cassette was introduced at the exact genomic site previously occupied by the target cassette. Through repeated RMCE using additional incompatible FRT sites, multiple groups of transgenes can be stacked at the same genomic locus.

    摘要翻译： 描述了靶向方法，其允许在先前表征的遗传基因座处进行精确的盒替换。 通过常规的生物弹性转化，将含有目标盒侧翼的一对不相容的FRT位点的靶DNA构建体引入大豆。 然后选择含有单个完整拷贝的靶位点的转基因事件，并用含有供体盒侧翼的相同对不对称FRT位点的供体DNA构建体重新转化。 通过重组酶介导的盒式交换（RMCE）在靶盒和供体盒之间发生精确的DNA盒交换，使得供体盒被导入到由靶盒预先占据的确切基因组位点。 通过反复RMCE使用额外的不相容的FRT位点，可以在相同的基因组座位堆叠多组转基因。

    Soybean event 3560.4.3.5 and compositions and methods for the identification and/or detection thereof
    39.
    发明申请
    Soybean event 3560.4.3.5 and compositions and methods for the identification and/or detection thereof 有权
    大豆事件3560.4.3.5及其鉴定和/或检测的组合物和方法

    公开(公告)号：US20090036308A1

    公开(公告)日：2009-02-05

    申请号：US12156182

    申请日：2008-05-30

    申请人： Anthony D. Guida, JR. ,  Christine B. Hazel ,  Jeffrey M. Hegstad ,  Zhongsen Li ,  Mark D. Vogt ,  Aiqiu Xing

    发明人： Anthony D. Guida, JR. ,  Christine B. Hazel ,  Jeffrey M. Hegstad ,  Zhongsen Li ,  Mark D. Vogt ,  Aiqiu Xing

    IPC分类号： A01N57/20 ,  A01P21/00

    CPC分类号： A01H5/10 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8278 ,  C12Q1/6895

    摘要： Compositions and methods related to transgenic glyphosate/ALS inhibitor-tolerant soybean plants are provided. Specifically, soybean plants having a 3560.4.3.5 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide are provided. The soybean plant harboring the 3560.4.3.5 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:10 and/or 11. The characterization of the genomic insertion site of the 3560.4.3.5 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean 3560.4.3.5 events are provided. Methods and compositions for increasing yield are also provided.

    摘要翻译： 提供了与转基因草甘膦/ ALS抑制剂耐受性大豆植物相关的组合物和方法。 具体地说，提供了赋予对草甘膦和至少一种ALS抑制性除草剂耐受性的3560.4.3.5事件的大豆植物。 在所述染色体位置处携带3560.4.3.5事件的大豆植物包含至少具有SEQ ID NO：10和/或11的多核苷酸序列的基因组/转基因接头.3560.4.3.5事件的基因组插入位点的表征提供 为了提高繁殖效率，并且能够使用分子标记来跟踪繁殖种群中的转基因插入物及其后代。 提供了用于鉴定，检测和使用大豆3560.4.3.5事件的各种方法和组合物。 还提供了用于提高产量的方法和组合物。