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公开(公告)号：US20090288217A1
公开(公告)日：2009-11-19
申请号：US12509538
申请日：2009-07-27
申请人： Zuo-Yu Zhao , Ning Wang
发明人： Zuo-Yu Zhao , Ning Wang
CPC分类号： A01H5/10
摘要： A novel double haploid maize line designated PHWVZ and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWVZ with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWVZ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWVZ or a trait conversion of PHWVZ with another maize line. Inbred maize lines derived from double haploid maize line PHWVZ, methods for producing other inbred maize lines derived from double haploid maize line PHWVZ and the inbred maize lines and their parts derived by the use of those method.
摘要翻译： 一种新型双倍体玉米品系，命名为PHWVZ及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍体单倍体玉米品系PHWVZ。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWVZ中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过双重单倍体系PHWVZ或PHWVZ与另一个玉米品系的转化而产生。 来自双倍体单倍体玉米品系PHWVZ的近交玉米品系，由双倍体单倍体玉米品系PHWVZ和近交玉米品系及其部分产生的其他近交玉米品系的生产方法。
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公开(公告)号：US20080244765A1
公开(公告)日：2008-10-02
申请号：US12062212
申请日：2008-04-03
申请人： Zuo-Yu Zhao , Youngzhong Wu , Michael Lassner , Howard Hershey
发明人： Zuo-Yu Zhao , Youngzhong Wu , Michael Lassner , Howard Hershey
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8216
摘要： Pollination process is disrupted by use of a pollination-disruption polynucleotide that renders the pollen unable to fertilize a sexually compatible ovule. The non-lethal nature of the pollen disruption polynucleotide is advantageous, particularly when operably linked to a transgenic polynucleotide of interest and prevents transmission of the polynucleotide through pollen. non-lethal markers are employed in an embodiment in which transgenic and non-transgenic seed can be sorted. A recombinase excision system is employed in an embodiment to activate the pollen disruption polynucleotide.
摘要翻译： 通过使用授粉破坏多核苷酸破坏授粉过程，使得花粉不能施用性别相容的胚珠。 花粉破坏多核苷酸的非致死性质是有利的，特别是当与感兴趣的转基因多核苷酸可操作地连接并且防止多核苷酸通过花粉传播时。 在其中可以分选转基因和非转基因种子的实施方案中采用非致死性标记。 在一个实施方案中采用重组酶切除系统来活化花粉破坏多核苷酸。
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公开(公告)号：US20080072344A1
公开(公告)日：2008-03-20
申请号：US11531801
申请日：2006-09-14
申请人： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
发明人： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
CPC分类号： A01H5/10
摘要： A novel inbred maize line designated PHWWE and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHWWE with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWE through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHWWE or a trait conversion of PHWWE with another maize line. Inbred maize lines derived from maize line PHWWE, methods for producing other inbred maize lines derived from inbred maize line PHWWE and the inbred maize lines and their parts derived by the use of those methods.
摘要翻译： 一种新型近交玉米品系被指定为PHWWE及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一玉米植物杂交近交玉米品系PHWWE。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWE中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分的方法。 杂交玉米种子，植物或植物部分通过交叉自交系PHWWE或PHWWE与另一种玉米品系的转化而产生。 衍生自玉米系PHWWE的近交玉米品系，由近交玉米系PHWWE生产其他近交玉米品系的方法以及通过使用这些方法得到的近交玉米品系及其成分。
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公开(公告)号：US20070220627A1
公开(公告)日：2007-09-20
申请号：US11559022
申请日：2006-11-13
IPC分类号： A01H5/00
CPC分类号： C12N15/8209
摘要： Compositions and methods are provided to screen, identify, select, isolate, and/or regenerate targeted integration events using seed priming. Seed priming provides the identification of a seed having stably incorporated into its genome a site-specific recombinase mediated integration of a selectable marker at a target locus operably linked to a promoter active in the seed.
摘要翻译： 提供组合和方法以使用种子启动来筛选，识别，选择，分离和/或再生目标整合事件。 种子引发提供了稳定地并入其基因组中的种子的鉴定，其位点特异性重组酶介导的可选择标记在可操作地连接到在种子中活化的启动子的靶基因座上的整合。
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公开(公告)号：US20070143880A1
公开(公告)日：2007-06-21
申请号：US11427371
申请日：2006-06-29
申请人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao
发明人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao
CPC分类号： C12N15/8205 , C12N15/8207 , C12N15/8213
摘要： The present invention provides methods and compositions which deliver Agrobacterium via microinjection directly into the embryo sac. At the time of injection, the embryo sac can comprise an egg cell, or alternatively, the embryo sac can be fertilized and comprise either a zygote or an embryo. Once inside the embryo sac, the Agrobacterium harboring a T-DNA having a polynucleotide of interest can express of the polynucleotide of interest in the plant. Further, the Agrobacterium can transfer the T-DNA having the polynucleotide of interest to the plant nucleus to produce a transformed plant. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and any tissue, plant part, and/or plant generated therefrom.
摘要翻译： 本发明提供了通过显微注射将土壤杆菌直接递送到胚囊中的方法和组合物。 在注射时，胚囊可以包括卵细胞，或者，胚囊可被受精并且包括受精卵或胚胎。 一旦进入胚囊，携带具有感兴趣的多核苷酸的T-DNA的农杆菌可以在植物中表达感兴趣的多核苷酸。 此外，农杆菌可以将具有目标多核苷酸的T-DNA转移到植物核，以产生转化的植物。 感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳的基因组中，以及由其产生的任何组织，植物部分和/或植物。
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公开(公告)号：US20060101540A1
公开(公告)日：2006-05-11
申请号：US11280890
申请日：2005-11-16
申请人： Jerome Ranch , Wallace Marsh , Dwight Tomes , Zuo-Yu Zhao
发明人： Jerome Ranch , Wallace Marsh , Dwight Tomes , Zuo-Yu Zhao
CPC分类号： C12N15/8202 , A01H1/04 , A01H5/10 , C12N15/8201 , C12N15/8205 , C12N15/8207 , C12N15/8209 , C12Q1/6855 , C12Q1/6895 , C12Q2600/156
摘要： Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.
摘要翻译： 提供了用于植物转化，提高转化效率和用于产生转基因植物的方法。 所述方法包括将受感染植物从感兴趣的植物物种的遗传系与选自相同植物物种的转基因遗传系或另一密切相关的植物物种的供体植物杂交以获得杂种植物。 从混合植物获得的组织是转化能力。 然后可以用一个或多个感兴趣的核苷酸序列转化这些组织，并选择用于整合在来自受体植物的染色体内的感兴趣的核苷酸序列的转基因事件。 可以选择转化的细胞，并再生转基因杂交植物。 感兴趣的核苷酸序列可以渗入原始受体亲本来源的基因系，或其他遗传系。 另外提供转化的植物和种子。
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37.发明授权公开(公告)号：US07022894B2
公开(公告)日：2006-04-04
申请号：US10784418
申请日：2004-02-23
CPC分类号： C12N15/8202 , A01H1/04 , A01H5/10 , C12N15/8201 , C12N15/8205 , C12N15/8207 , C12N15/8209 , C12Q1/6855 , C12Q1/6895 , C12Q2600/156
摘要： Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.
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