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公开(公告)号：US08143484B2
公开(公告)日：2012-03-27
申请号：US12357284
申请日：2009-01-21
申请人： Luke Mehlo , Zuo-Yu Zhao
发明人： Luke Mehlo , Zuo-Yu Zhao
IPC分类号： C12N15/84
CPC分类号： C12N15/8205
摘要： A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium, effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.
摘要翻译： 描述了调节农杆菌毒力基因表达的方法。 该方法包括刺激谷类细胞如高粱的步骤，以产生活性的，通常为酚的化合物，并将土壤杆菌暴露于该化合物。 该化合物诱导土壤杆菌的毒力基因的表达，从而转化从土壤杆菌到谷物细胞的T-DNA转移。
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公开(公告)号：US20100192253A1
公开(公告)日：2010-07-29
申请号：US12694013
申请日：2010-01-26
申请人： XINLI E. WU , MYEONG-JE CHO , ZUO-YU ZHAO
发明人： XINLI E. WU , MYEONG-JE CHO , ZUO-YU ZHAO
CPC分类号： C12N15/8205
摘要： The invention provides improved plant transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method includes various transformation protocols for monocots, such as maize and sorghum, using a combination of media and light conditions to achieve increased efficiency of monocot transformation and increased callus initiation frequencies.
摘要翻译： 本发明提供改进的植物转化方法。 特别地，该方法提供增加的转化频率，特别是在顽固植物中。 该方法包括用于单子叶植物（例如玉米和高粱）的各种转化方案，其使用培养基和光照条件的组合来实现提高单子叶植物转化的效率和增加的愈伤组织起始频率。
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公开(公告)号：US06720475B1
公开(公告)日：2004-04-13
申请号：US09641111
申请日：2000-08-17
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC分类号： A01H500
CPC分类号： C12N15/8289 , C12N9/00 , C12N15/8213
摘要： A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译： 在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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公开(公告)号：US08030556B2
公开(公告)日：2011-10-04
申请号：US12174812
申请日：2008-07-17
申请人： Zuo-Yu Zhao , Ning Wang , Douglas S. Nubel , Shifu Zhen
发明人： Zuo-Yu Zhao , Ning Wang , Douglas S. Nubel , Shifu Zhen
CPC分类号： A01H5/10
摘要： A novel double haploid maize line designated PH17AW and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PH17AW with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH17AW through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PH17AW or a trait conversion of PH17AW with another maize line. Inbred maize lines derived from double haploid maize line PH17AW, methods for producing other inbred maize lines derived from double haploid maize line PH17AW and the inbred maize lines and their parts derived by those methods.
摘要翻译： 一种新型双倍体玉米品系，命名为PH17AW，其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍单倍体玉米品系PH17AW。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗入PH17AW的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过双重单倍体系PH17AW或PH17AW与另一个玉米品系的转化而产生。 衍生自双倍体单倍体玉米品系PH17AW的近交玉米品系，从双倍体单倍体玉米品系PH17AW生产其他近交玉米品系的方法和由这些方法衍生的近交玉米品系及其部分。
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公开(公告)号：US07999158B2
公开(公告)日：2011-08-16
申请号：US12534507
申请日：2009-08-03
申请人： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
发明人： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
CPC分类号： A01H5/10
摘要： A novel inbred maize line designated PHWWE and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHWWE with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWE through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHWWE or a trait conversion of PHWWE with another maize line. Inbred maize lines derived from maize line PHWWE, methods for producing other inbred maize lines derived from inbred maize line PHWWE and the inbred maize lines and their parts derived by the use of those methods.
摘要翻译： 一种新型近交玉米品系被指定为PHWWE及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一玉米植物杂交近交玉米品系PHWWE。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWE中的一种或多种性状，以及由此生产的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过交叉自交系PHWWE或PHWWE与另一种玉米品系的转化而产生。 衍生自玉米系PHWWE的近交玉米品系，由近交玉米系PHWWE生产其他近交玉米品系的方法以及通过使用这些方法得到的近交玉米品系及其成分。
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公开(公告)号：US20100154083A1
公开(公告)日：2010-06-17
申请号：US12709630
申请日：2010-02-22
CPC分类号： H02J7/0042 , F21S9/02 , F21V33/00 , F21W2131/1005
摘要： Compositions and methods are provided to screen, identify, select, isolate, and/or regenerate targeted integration events using seed priming. Seed priming provides the identification of a seed having stably incorporated into its genome a site-specific recombinase mediated integration of a selectable marker at a target locus operably linked to a promoter active in the seed.
摘要翻译： 提供组合和方法以使用种子启动来筛选，识别，选择，分离和/或再生目标整合事件。 种子引发提供了稳定地并入其基因组中的种子的鉴定，其位点特异性重组酶介导的可选择标记在可操作地连接到在种子中活化的启动子的靶基因座上的整合。
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公开(公告)号：US20090263902A1
公开(公告)日：2009-10-22
申请号：US12357284
申请日：2009-01-21
申请人： Luke MEHLO , Zuo-Yu Zhao
发明人： Luke MEHLO , Zuo-Yu Zhao
CPC分类号： C12N15/8205
摘要： A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium, effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.
摘要翻译： 描述了调节农杆菌毒力基因表达的方法。 该方法包括刺激谷类细胞如高粱的步骤，以产生活性的，通常为酚的化合物，并将土壤杆菌暴露于该化合物。 该化合物诱导土壤杆菌的毒力基因的表达，从而转化从土壤杆菌到谷物细胞的T-DNA转移。
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公开(公告)号：US20080299658A1
公开(公告)日：2008-12-04
申请号：US11755083
申请日：2007-05-30
申请人： Zuo-Yu Zhao , Ning Wang
发明人： Zuo-Yu Zhao , Ning Wang
CPC分类号： A01H5/10
摘要： A novel double haploid maize line designated PHWVZ and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWVZ with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWVZ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWVZ or a trait conversion of PHWVZ with another maize line. Inbred maize lines derived from double haploid maize line PHWVZ, methods for producing other inbred maize lines derived from double haploid maize line PHWVZ and the inbred maize lines and their parts derived by the use of those method.
摘要翻译： 一种新型双倍体玉米品系，命名为PHWVZ及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍体单倍体玉米品系PHWVZ。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWVZ中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过双重单倍体系PHWVZ或PHWVZ与另一个玉米品系的转化而产生。 来自双倍体单倍体玉米品系PHWVZ的近交玉米品系，由双倍体单倍体玉米品系PHWVZ和近交玉米品系及其部分产生的其他近交玉米品系的生产方法。
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9.发明申请Methods of Transforming Plants and Identifying Parental Origin of a Chromosome In Those Plants 审中-公开在这些植物中转化植物和识别染色体的亲本起源的方法公开(公告)号：US20080131896A1
公开(公告)日：2008-06-05
申请号：US11941106
申请日：2007-11-16
申请人： Jerome P. Ranch , Wallace A. Marsh , Terry EuClaire Meyer , Dwight T. Tomes , Zuo-Yu Zhao , Gan-Yuan Zhong , Jimei Wang , Jeffery A. Mullen , Christopher J. Scelonge , Glenn S. Cole , John F. Soper
发明人： Jerome P. Ranch , Wallace A. Marsh , Terry EuClaire Meyer , Dwight T. Tomes , Zuo-Yu Zhao , Gan-Yuan Zhong , Jimei Wang , Jeffery A. Mullen , Christopher J. Scelonge , Glenn S. Cole , John F. Soper
IPC分类号： C12Q1/68
CPC分类号： C12N15/8202 , A01H1/04 , A01H5/10 , C12N15/8201 , C12N15/8205 , C12N15/8207 , C12N15/8209 , C12Q1/6855 , C12Q1/6895 , C12Q2600/156
摘要： Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.
摘要翻译： 提供了用于植物转化，提高转化效率和用于产生转基因植物的方法。 所述方法包括将受感染植物从感兴趣的植物物种的遗传系与选自相同植物物种的转基因遗传系或另一密切相关的植物物种的供体植物杂交以获得杂种植物。 从混合植物获得的组织是转化能力。 然后可以用一个或多个感兴趣的核苷酸序列转化这些组织，并选择用于整合在来自受体植物的染色体内的感兴趣的核苷酸序列的转基因事件。 可以选择转化的细胞，并再生转基因杂交植物。 感兴趣的核苷酸序列可以渗入原始受体亲本来源的基因系，或其他遗传系。 另外提供转化的植物和种子。
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公开(公告)号：US20060185033A1
公开(公告)日：2006-08-17
申请号：US11298973
申请日：2005-12-09
申请人： Zuo-yu Zhao , Dennis Bidney , Evan Elsing , Michael Miller , Xinli Wu , William Gordon-Kamm
发明人： Zuo-yu Zhao , Dennis Bidney , Evan Elsing , Michael Miller , Xinli Wu , William Gordon-Kamm
CPC分类号： C12N15/8241 , A01H5/10 , C12N15/8201 , C12N15/829
摘要： Methods for producing homozygous plants, seeds, and plant cells are provided. Methods of forming haploid tissue and then doubling the chromosomes to form doubled haploid cells are provided. Also provided are methods of transformation.
摘要翻译： 提供了用于产生纯合植物，种子和植物细胞的方法。 提供了形成单倍体组织，然后使染色体加倍以形成双倍体单倍体细胞的方法。 还提供了转化的方法。
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