HIGH AMYLOSE WHEAT - II
    37.
    发明申请
    HIGH AMYLOSE WHEAT - II 审中-公开
    高含量的燕麦 - II

    公开(公告)号:US20170064986A1

    公开(公告)日:2017-03-09

    申请号:US15156066

    申请日:2016-05-16

    IPC分类号: A23L7/10 B02C9/00

    摘要: Wheat grain (Triticum aestivum) comprising an embryo and starch, wherein the embryo comprises two identical alleles of an SBEIIa-A gene, two identical alleles of an SBEIIa-B gene and two identical alleles of an SBEIIa-D gene, wherein each of the SBEIIa genes gives rise to an amount of protein (w/w) or a protein having SBEIIa activity which is lower than the corresponding wild-type gene, and at least one of said genes comprises a point mutation, wherein the starch comprises amylose such that the grain has an amylose content of at least 50% (w/w) as a proportion of the extractable starch of the grain.

    摘要翻译: 包含胚胎和淀粉的小麦籽粒(Triticum aestivum),其中胚胎包含SBEIIa-A基因的两个相同的等位基因,SBEIIa-B基因的两个相同的等位基因和SBEIIa-D基因的两个相同的等位基因,其中 SBEIIa基因产生一定量的蛋白质(w / w)或具有低于相应野生型基因的SBEIIa活性的蛋白质,并且所述基因中的至少一个包含点突变,其中淀粉包含直链淀粉,使得 颗粒具有至少50%(w / w)的直链淀粉含量作为颗粒的可提取淀粉的比例。

    Barley and uses thereof
    39.
    发明授权

    公开(公告)号:US10154632B2

    公开(公告)日:2018-12-18

    申请号:US13387973

    申请日:2010-07-30

    摘要: The invention provides barley gain comprising a reduced level or activity of starch synthase IIa protein and a starch content of at least 41% (w/w) and methods of producing, identifying and using same. The grain may comprise an amylase content of at least 50%, a β-glucan content of 5-9% (w/w) or greater than 9% (w/w), and/or a fructan content of 3-11% (w/w). The fructan may comprise a degree of polymerization from about 3 to about 12. For example, the plant and grain comprises a sex6-292 allele and/or an amo1 mutation. A food or beverage product, and methods of producing a food or beverage product, comprising obtaining or producing the subject grain and processing the grain to produce the product. Also contemplated are methods of improving one or more indicators of health in a mammal comprising administering a composition comprising the subject barley grain or a product comprising same.

    Plants with modified starch metabolism

    公开(公告)号:US10100324B2

    公开(公告)日:2018-10-16

    申请号:US12745237

    申请日:2008-11-27

    摘要: The specification provides methods of obtaining a genetically modified plant which has increased production potential compared to a control plant, the method comprising the steps of i) obtaining a plurality of plants at least one of which comprises in its genome a heterologous polynucleotide, ii) identifying from the plurality of plants a plant which has increased production potential relative to the control plant and comprises the heterologous polynucleotide, and iii) selecting the genetically modified plant, wherein the polynucleotide comprises a transcriptional control sequence operably linked to a nucleic acid sequence which encodes an agent that modifies endogenous starch phosphorylation and/or starch degradation in the plant. In some embodiments, the plant has increased endogenous glycosylase or increased digestibility compared to a control plant. In some specific embodiments, the endogenous starch phosphorylation and/or starch degradation is modified by modifying expression or activity of one or more enzymes selected from the group consisting of α-amylase (EC 3.2.1.1), β-amylase (EC 3.2.1.2), glucoamylase (EC 3.2.1.3), starch phosphorylase (EC2.4.1.1), glycosylase (EC 3.1.33), sucrase-isomaltase (EC 3.2.10), amylomaltase (EC 2.4.1.25), maltase (EC 3.2.1.20), isoamylase, and α-glucan, water dikinase (GWD, EC 2.7.9.4).