公开(公告)号：US12077765B2

公开(公告)日：2024-09-03

申请号：US16750349

申请日：2020-01-23

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James A. Strickland ,  Marcos Fernando de Godoy Lusso

IPC: C12N15/82

CPC classification number: C12N15/8243

Abstract: The present disclosure provides approaches for reducing nicotine. Also provided are tobacco plants with decreased tobacco-specific nitrosamines (TSNAs). The present disclosure further provides modified tobacco plants with low nicotine and increased antioxidant capacity. Further provided are tobacco plants or material with low nicotine and low TSNAs. Also provided is cured tobacco material of the tobacco plants provided herein and tobacco products comprising this cured tobacco material.

公开(公告)号：US20230329168A1

公开(公告)日：2023-10-19

申请号：US18170372

申请日：2023-02-16

Applicant: Altria Client Services LLC

Inventor： Andrew Carl Adams ,  Marcos Fernando de Godoy Lusso ,  Sreepriya Pramod ,  Dongmei Xu

IPC: A24B15/10 ,  A01H5/12 ,  A24B13/00 ,  C12N15/82 ,  A01H6/82 ,  A24B3/12 ,  A24B13/02 ,  A24D1/04

CPC classification number: A24B15/10 ,  A01H5/12 ,  A24B13/00 ,  C12N15/8243 ,  A01H6/823 ,  A24B3/12 ,  A24B13/02 ,  A24D1/045

Abstract: The present disclosure provides the identification of tobacco Nic1 locus. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Also provided are compositions and methods for producing tobacco plants having novel Nic1 mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or nicotine free while maintaining tobacco leaf grade and tobacco product quality.

公开(公告)号：US11690347B2

公开(公告)日：2023-07-04

申请号：US17704650

申请日：2022-03-25

Applicant: Altria Client Services LLC

Inventor： Andrew C. Adams ,  Marcos F. de Godoy Lusso ,  Sreepriya Pramod ,  Dongmei Xu

IPC: A01H5/12 ,  A01H6/82

CPC classification number: A01H6/823 ,  A01H5/12

Abstract: The present application provides tobacco inbred plants dS1746, dS1746MS, dS1564 and dS1564MS. The present application also provides parts of such plants and products made from those parts. The present application also includes progeny of the provided plants including hybrids.

公开(公告)号：US11602118B2

公开(公告)日：2023-03-14

申请号：US17116816

申请日：2020-12-09

Applicant: Altria Client Services LLC

Inventor： Jesse Frederick ,  Chengalrayan Kudithipudi ,  Dongmei Xu

IPC: C12N15/82 ,  A01H5/12 ,  A01H6/82 ,  C07K14/415 ,  C12N9/90 ,  C12N9/10 ,  C12N9/88 ,  A01H5/10

Abstract: The present disclosure provides metabolic signatures and genetic markers for tracking enhanced nitrogen utilization efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen utilization efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen utilization efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen utilization efficiency. The disclosure also provides recombinant polynucleotides and polypeptides for enhancing nitrogen utilization efficiency in modified tobacco plants and tobacco plants comprising the provided recombinant polynucleotides and polypeptides.

公开(公告)号：US11384361B2

公开(公告)日：2022-07-12

申请号：US16523326

申请日：2019-07-26

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan Kudithipudi ,  Dong Qi ,  Yanxin Shen ,  Ujwala Warek ,  James A. Strickland ,  Dongmei Xu

IPC: A01H5/12 ,  C12N15/00 ,  C12N15/82 ,  A01H6/82 ,  C12Q1/6827

Abstract: The present disclosure provides compositions and methods related to tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

公开(公告)号：US11377665B2

公开(公告)日：2022-07-05

申请号：US16372833

申请日：2019-04-02

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James Strickland ,  Yanxin Shen

IPC: C12N15/82 ,  A24B15/10 ,  C12N9/88 ,  A24B13/00 ,  A24B15/24 ,  A24B15/20

Abstract: The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), increase phenylalanine, or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs. Also provided are methods and compositions for increasing the expression of chorismate mutase and other transcription factors involved in anthocyanin biosynthesis.

公开(公告)号：US11293030B2

公开(公告)日：2022-04-05

申请号：US16529365

申请日：2019-08-01

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Jaemo Yang ,  Yanxin Shen ,  Dongmei Xu ,  Ujwala Warek ,  James Strickland ,  Jesse Frederick

IPC: C12N15/82

Abstract: The present disclosure provides the identification of promoters that are preferentially active in tobacco axillary buds. Also provided are tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing tobacco plants comprising reduced or no sucker growth.

公开(公告)号：US10888064B2

公开(公告)日：2021-01-12

申请号：US16119366

申请日：2018-08-31

Applicant: Altria Client Services LLC

Inventor： Jesse Frederick ,  Chengalrayan Kudithipudi ,  Dongmei Xu

IPC: A01H5/10 ,  C12N15/82 ,  A01H6/82 ,  C12N9/90 ,  C07K14/415 ,  A01H5/12 ,  C12N9/10 ,  C12N9/88

Abstract: The present disclosure provides metabolic signatures and genetic markers for tracking enhanced nitrogen utilization efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen utilization efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen utilization efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen utilization efficiency. The disclosure also provides recombinant polynucleotides and polypeptides for enhancing nitrogen utilization efficiency in modified tobacco plants and tobacco plants comprising the provided recombinant polynucleotides and polypeptides.

公开(公告)号：US10731173B2

公开(公告)日：2020-08-04

申请号：US15457553

申请日：2017-03-13

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Dongmei Xu ,  Chengalrayan Kudithipudi ,  Yanxin Shen ,  Jaemo Yang ,  Jesse Frederick ,  James Strickland

IPC: C12N15/82 ,  C07K14/415 ,  A24B13/00 ,  A24B15/10

Abstract: The present disclosure provides the identification of genes involved in sucker growth in tobacco. Also provided are promoters that are preferentially active in tobacco axillary buds. Also provided are modified tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing modified tobacco plants comprising reduced or no sucker growth.

公开(公告)号：US20200239899A1

公开(公告)日：2020-07-30

申请号：US16750349

申请日：2020-01-23

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James A. Strickland ,  Marcos Fernando de Godoy Lusso

IPC: C12N15/82

Abstract: The present disclosure provides approaches for reducing nicotine. Also provided are tobacco plants with decreased tobacco-specific nitrosamines (TSNAs). The present disclosure further provides modified tobacco plants with low nicotine and increased antioxidant capacity. Further provided are tobacco plants or material with low nicotine and low TSNAs. Also provided is cured tobacco material of the tobacco plants provided herein and tobacco products comprising this cured tobacco material.