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公开(公告)号：US6025188A
公开(公告)日：2000-02-15
申请号：US888949
申请日：1997-07-07
申请人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang , Benjamin A. Bowen , Jacob T. Gilliam
发明人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang , Benjamin A. Bowen , Jacob T. Gilliam
IPC分类号： C12N15/09 , A01N63/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/00 , C12N15/31 , C12N15/55 , C12N15/82 , C12R1/645 , C12S3/00 , A23L1/015
CPC分类号： A01N63/00 , C12N15/8209 , C12N15/8242 , C12N15/8282 , C12N9/00 , C12N9/16 , C12N9/18
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
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32.发明授权公开(公告)号：US08735158B2
公开(公告)日：2014-05-27
申请号：US12039307
申请日：2008-02-28
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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公开(公告)号：US07462766B2
公开(公告)日：2008-12-09
申请号：US11417588
申请日：2006-05-04
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Compositions comprising non-identical recombination sites are provided. Compositions include plants, plant parts and plant cells comprising transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites.
摘要翻译： 提供了包含非相同重组位点的组合物。 组合物包括植物，植物部分和植物细胞，其包含转移盒，其包含侧翼为不相同重组位点的感兴趣的核苷酸序列。
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34.发明授权Compositions and methods to reduce the complexity of transgene integration into the genome of a plant 有权用于减少转基因整合到植物基因组中的复杂性的组合物和方法公开(公告)号：US07405079B2
公开(公告)日：2008-07-29
申请号：US10430908
申请日：2003-05-07
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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35.发明授权公开(公告)号：US07361508B2
公开(公告)日：2008-04-22
申请号：US10440030
申请日：2003-05-16
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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36.发明授权Compositions and methods to reduce the complexity of transgene integration in the genome of a plant 有权减少植物基因组中转基因整合的复杂性的组合物和方法公开(公告)号：US06573425B1
公开(公告)日：2003-06-03
申请号：US09439042
申请日：1999-11-12
IPC分类号： A01H100
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods for reducing the complexity of integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了降低将核苷酸序列整合到植物中的复杂性的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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37.发明授权Compositions and methods for locating preferred integration sites within a plant genome 有权用于定位植物基因组内优选整合位点的组合物和方法公开(公告)号：US06552248B1
公开(公告)日：2003-04-22
申请号：US09438239
申请日：1999-11-12
IPC分类号： A01H500
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods to find optimal integration sites within a plant genome are provided. More particularly, a plant is transformed with a target site having an expression cassette comprising a nucleotide sequence operably linked to a promoter active in the plant. The target site is flanked by non-identical recombination sites, Transformed protoplast, tissues, or whole plants can be tested to determine the levels of activity of the inserted gene, By comparison of cellular activities of the gene in different insertion sites, preferred integration sites may be found wherein the gene is expressed at high or acceptable levels. These plants can then be utilized with subsequent retargeting techniques to replace the nucleotide sequence with other genes or nucleotide sequences of interest contained in a transfer cassette.
摘要翻译： 提供了在植物基因组内找到最佳整合位点的方法。 更具体地，用具有表达盒的靶位点转化植物，所述表达盒包含可操作地连接于在植物中有活性的启动子的核苷酸序列。 靶位点侧翼为不相同的重组位点，可以测试转化的原生质体，组织或整个植物以确定插入的基因的活性水平。通过比较不同插入位点中基因的细胞活性，优选的整合位点 可以发现其中基因以高或可接受的水平表达。 然后可以利用这些植物与随后的重定靶技术将核苷酸序列替换为包含在转移盒中的其它感兴趣的基因或核苷酸序列。
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38.发明授权Compositions and methods for the targeted removal of a nucleotide sequence from the genome of a plant 有权用于从植物基因组靶向去除核苷酸序列的组合物和方法公开(公告)号：US06458594B1
公开(公告)日：2002-10-01
申请号：US09439158
申请日：1999-11-12
IPC分类号： A01H500
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions to remove a nucleotide sequence of interest in a plant and plant cell are provided. In particular the methods of the invention comprise providing a plant cell having stably incorporated into its genome a transfer cassette comprising a nucleotide sequence of interest flanked by non-identical recombination sites and introducing into the plant cell a chimeric RNA-DNA oligonucleotide molecule. The chimeric RNA-DNA oligonucleotide is capable of recognizing and implementing a nucleotide conversion in one of the non-identical recombination sites so as to create two identical recombination sites. An appropriate recombinase is provided which excises the sequences between the two identical recombination sites.
摘要翻译： 提供了在植物和植物细胞中去除目标核苷酸序列的方法和组合物。 特别地，本发明的方法包括提供在其基因组中稳定并入转移盒的植物细胞，转移盒包含感兴趣的核苷酸序列，侧翼为不相同的重组位点，并将嵌合的RNA-DNA寡核苷酸分子引入植物细胞。 嵌合RNA-DNA寡核苷酸能够识别并实现其中一个非相同重组位点的核苷酸转化，从而产生两个相同的重组位点。 提供了适当的重组酶，其在两个相同的重组位点之间切除序列。
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公开(公告)号：US06291224B1
公开(公告)日：2001-09-18
申请号：US09677064
申请日：2000-09-29
IPC分类号： C12N988
CPC分类号： C12N9/1205 , C12N9/90 , C12N15/8243
摘要： This invention relates to newly identified polynucleotides and polypeptides, variants and derivatives of same; methods for making the polynucleotides, polypeptides, variants, derivatives and antagonists. In particular the invention relates to polynucleotides and polypeptides of the phytate metabolic pathway.
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公开(公告)号：US06187994B1
公开(公告)日：2001-02-13
申请号：US09193502
申请日：1998-11-17
IPC分类号： C12N504
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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