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公开(公告)号：US06670189B2
公开(公告)日：2003-12-30
申请号：US09731393
申请日：2000-12-05
申请人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang , Benjamin A. Bowen , Jacob T. Gilliam
发明人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang , Benjamin A. Bowen , Jacob T. Gilliam
IPC分类号： C12N1582
CPC分类号： A01N63/00 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/8209 , C12N15/8242 , C12N15/8282
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
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公开(公告)号：US6025188A
公开(公告)日：2000-02-15
申请号：US888949
申请日：1997-07-07
申请人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang , Benjamin A. Bowen , Jacob T. Gilliam
发明人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang , Benjamin A. Bowen , Jacob T. Gilliam
IPC分类号： C12N15/09 , A01N63/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/00 , C12N15/31 , C12N15/55 , C12N15/82 , C12R1/645 , C12S3/00 , A23L1/015
CPC分类号： A01N63/00 , C12N15/8209 , C12N15/8242 , C12N15/8282 , C12N9/00 , C12N9/16 , C12N9/18
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
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3.发明授权Amino polyol oxidase amine polynucleotides and related polypeptides and methods of use 失效氨基多胺胺氧化酶胺多核苷酸及相关多肽及其使用方法公开(公告)号：US06835569B2
公开(公告)日：2004-12-28
申请号：US09770564
申请日：2001-01-26
IPC分类号： C12N1509
CPC分类号： C12N9/0022 , A23K10/14 , A23K10/18 , A23K30/18 , C07K2319/00 , C12N9/16 , C12N15/8209 , C12N15/8242 , C12N15/8282 , G01N2333/906
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems.
摘要翻译： 本发明提供了从Exophiala spinifera分离的酶APAO的多核苷酸和相关多肽。 此外，编码APAO酶的多核苷酸可用于转化通常易受镰刀菌或其它产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 另外，本发明提供在转基因植物中表达APAO和伏马菌素酯酶。 以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。 此外，本发明提供了在原核和非植物真核系统中生产APAO酶的方法。
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4.发明授权Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法公开(公告)号：US06211435B1
公开(公告)日：2001-04-03
申请号：US09352168
申请日：1999-07-12
IPC分类号： C12N504
CPC分类号： C12N9/0022 , A23K10/14 , A23K10/18 , A23K30/18 , C07K2319/00 , C12N9/16 , C12N15/8209 , C12N15/8242 , C12N15/8282 , G01N2333/906
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
摘要翻译： 本发明提供了从Exophiala spinifera分离的酶APAO的多核苷酸和相关多肽。 此外，编码APAO酶的多核苷酸可用于转化通常易受镰刀菌或其它产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 另外，本发明提供在转基因植物中表达APAO和伏马菌素酯酶。 以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。 此外，本发明提供了在原核和非植物真核系统中生产APAO酶的方法。 还公开了谷物，谷物加工，青贮饲料，粮食作物，动物饲料和瘤胃微生物的解毒方法。
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公开(公告)号：US06812380B2
公开(公告)日：2004-11-02
申请号：US10107096
申请日：2002-03-26
IPC分类号： C12N1509
CPC分类号： C12N9/18
摘要： The present invention provides nucleotide sequences encoding zearalenone detoxification polypeptides, and methods for mycotoxin detoxification using the sequences. One method comprises stably incorporating into the genome of a plant cell, a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译： 本发明提供了编码玉米赤霉烯酮解毒多肽的核苷酸序列，以及使用该序列的霉菌毒素解毒的方法。 一种方法包括稳定地并入植物细胞的基因组中，本发明的核苷酸序列可操作地连接到异源启动子并再生表达核苷酸序列的稳定转化的植物。
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6.发明授权Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 失效氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法公开(公告)号：US06943279B1
公开(公告)日：2005-09-13
申请号：US09658835
申请日：2000-09-08
申请人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Aragula Gururaj Rao , Oswald R. Crasta , Otto Folkerts
发明人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Aragula Gururaj Rao , Oswald R. Crasta , Otto Folkerts
CPC分类号： C12N9/0022 , C12N15/8242 , C12N15/8282
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella airovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.
摘要翻译： 本发明提供了从Exophiala spinifera和Rhinocladiella airovirens分离的酶APAO的多核苷酸和相关多肽。 可以突变多核苷酸以除去糖基化位点和半胱氨酸残基。 另外，本发明提供了重组表达盒，宿主细胞，转基因植物和转基因种子。 本发明还提供了含有APAO和伏马菌素酯酶的多核苷酸。 此外，本发明提供了在原核和真核系统中生产APAO酶的方法，用于检测伏马毒素的方法和用于鉴定转化的植物细胞的方法。 还公开了降解植物，谷物，谷物加工，青贮饲料，粮食作物和动物饲料中真菌毒素的方法。
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7.发明授权Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法公开(公告)号：US07241934B2
公开(公告)日：2007-07-10
申请号：US10743891
申请日：2003-12-22
申请人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Aragula Gururaj Rao , Oswald R. Crasta , Otto Folkerts
发明人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Aragula Gururaj Rao , Oswald R. Crasta , Otto Folkerts
CPC分类号： C12N9/0022 , C12N15/8242 , C12N15/8282
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.
摘要翻译： 本发明提供了从Exophiala spinifera和Rhinocladiella atvirens分离的酶APAO的多核苷酸和相关多肽。 可以突变多核苷酸以除去糖基化位点和半胱氨酸残基。 另外，本发明提供了重组表达盒，宿主细胞，转基因植物和转基因种子。 本发明还提供了含有APAO和伏马菌素酯酶的多核苷酸。 此外，本发明提供了在原核和真核系统中生产APAO酶的方法，用于检测伏马毒素的方法和用于鉴定转化的植物细胞的方法。 还公开了降解植物，谷物，谷物加工，青贮饲料，粮食作物和动物饲料中真菌毒素的方法。
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8.发明授权Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 失效氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法公开(公告)号：US06987212B2
公开(公告)日：2006-01-17
申请号：US10624619
申请日：2003-07-22
CPC分类号： C12N9/0022 , A23K10/14 , A23K10/18 , A23K30/18 , C07K2319/00 , C12N9/16 , C12N15/8209 , C12N15/8242 , C12N15/8282 , G01N2333/906
摘要： The present invention provides transformed plants comprising a polynucleotide enconding amino polyol amine oxidase (APAO ) from Exophiala spinifera and a method of expressing APAO transgenic plants and seeds. In this way, transgenic plant can be produced having fumonisin degrading capability, as well as with the capability of producing the degrading enzymes.
摘要翻译： 本发明提供了转化植物，其包含包含来自外来叶柄菌的氨基多元胺胺氧化酶（APAO）的多核苷酸和表达APAO转基因植物和种子的方法。 以这种方式，可以生产具有伏马菌素降解能力的转基因植物以及产生降解酶的能力。
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9.发明授权Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 失效氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法公开(公告)号：US06737562B1
公开(公告)日：2004-05-18
申请号：US09771045
申请日：2001-01-26
IPC分类号： C12N1509
CPC分类号： C12N9/0022 , A23K10/14 , A23K10/18 , A23K30/18 , C07K2319/00 , C12N9/16 , C12N15/8209 , C12N15/8242 , C12N15/8282 , G01N2333/906
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinfera and Rhinocladiella atrovirens. Additionally, the polynucleotides encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium. Plants can be regenerated from the transformed plant cells.
摘要翻译： 本发明提供了从Exophiala spinfera和Rhinocladiella atrovirens分离的酶APAO的多核苷酸和相关多肽。 此外，编码APAO酶的多核苷酸可用于转化通常易受镰刀菌感染的植物细胞。 植物可以从转化的植物细胞中再生。
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10.发明授权Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法公开(公告)号：US06211434B1
公开(公告)日：2001-04-03
申请号：US09352159
申请日：1999-07-12
IPC分类号： C12N504
CPC分类号： C12N9/0022 , A23K10/14 , A23K10/18 , A23K30/18 , C07K2319/00 , C12N9/16 , C12N15/8209 , C12N15/8242 , C12N15/8282 , G01N2333/906
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. Additionally, the polynucleotides encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
摘要翻译： 本发明提供了从Exophiala spinifera和Rhinocladiella atvirens分离的酶APAO的多核苷酸和相关多肽。 此外，编码APAO酶的多核苷酸可用于转化通常易受镰刀菌或其它产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 另外，本发明提供在转基因植物中表达APAO和伏马菌素酯酶。 以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。 此外，本发明提供了在原核和非植物真核系统中生产APAO酶的方法。 还公开了谷物，谷物加工，青贮饲料，粮食作物，动物饲料和瘤胃微生物的解毒方法。
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