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    Novel human polynucleotides and polypeptides encoded thereby
    34.
    发明申请
    Novel human polynucleotides and polypeptides encoded thereby 审中-公开
    新的人多核苷酸和由此编码的多肽

    公开(公告)号：US20070178475A1

    公开(公告)日：2007-08-02

    申请号：US11128224

    申请日：2005-05-12

    申请人： Michael Nehls ,  Brian Zambrowicz ,  Arthur Sands

    发明人： Michael Nehls ,  Brian Zambrowicz ,  Arthur Sands

    IPC分类号： C12Q1/68 ,  G06F19/00 ,  C07H21/04

    CPC分类号： C07H21/04

    摘要： Novel human polynucleotides are disclosed that correspond to human gene trapped sequences, or GTSs. The disclosed GTSs are useful for gene discovery and as markers for, inter alia, gene expression analysis, identifying and mapping the coding regions of the mammalian, and particularly human, genome, forensic analysis, and determining the genetic basis of human disease.

    摘要翻译： 公开了对应于人基因捕获序列或GTS的新型人多核苷酸。 所公开的GTS可用于基因发现，并且用作基因表达分析，鉴定和绘制哺乳动物编码区，特别是人基因组，法医分析和确定人类疾病遗传基础的标记。

    Indexed library of cells containing genomic modifications and methods of making and utilizing the same
    38.
    发明申请
    Indexed library of cells containing genomic modifications and methods of making and utilizing the same 有权
    指定的含有基因组修饰的细胞库，以及制备和利用它们的方法

    公开(公告)号：US20050095620A1

    公开(公告)日：2005-05-05

    申请号：US10917273

    申请日：2004-08-11

    申请人： Brian Zambrowicz ,  Glenn Friedrich ,  Allan Bradley ,  Arthur Sands

    发明人： Brian Zambrowicz ,  Glenn Friedrich ,  Allan Bradley ,  Arthur Sands

    IPC分类号： C12N15/10 ,  C12N15/85 ,  C12N15/867 ,  C12Q1/68 ,  A01K67/027 ,  C12N5/06 ,  G01N33/53 ,  G01N33/567

    CPC分类号： C12N15/86 ,  A01K2217/075 ,  C12N15/102 ,  C12N15/1051 ,  C12N15/1065 ,  C12N15/1086 ,  C12N15/85 ,  C12N2740/13043 ,  C12N2800/60 ,  C12N2840/203 ,  C12N2840/44

    摘要： Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the genome of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.

    摘要翻译： 提供方法和载体（DNA和逆转录病毒）用于构建突变细胞库。 该文库优选地在基因组中存在基因组中所有基因的突变。 图书馆和载体的性质允许筛选特定基因突变的方法，并收集每个突变基因的核苷酸序列数据，以提供标记基因序列的数据库。 这样的数据库提供了访问图书馆中包含的各个突变细胞克隆的方法。 本发明包括描述的库，制作方法和用于构建库的向量。 还提供了通过序列或汇总和筛选来访问图书馆的各个部分的方法。 本发明还提供了从文库细胞中分离并产生的特异性基因突变的非人转基因动物的产生。