公开(公告)号：US20160304895A1

公开(公告)日：2016-10-20

申请号：US15097349

申请日：2016-04-13

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Daren Hemingway ,  Carla Ausmus ,  Andrew F. Worden ,  Andrew Asberry

IPC: C12N15/82

CPC classification number: C12N15/8227 ,  C07K14/825 ,  C12N15/8216

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Panicum virgatum metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.

Abstract translation: 本公开涉及使用来自黍属金丝桃素样基因（mt1）的启动子来促进植物或植物细胞中核苷酸序列转录的组合物和方法。 一些实施方案涉及在植物中起作用以促进可操作地连接的核苷酸序列的转录的来自Panicum virgatum金属硫蛋白样基因（mt1）的启动子。

公开(公告)号：US20150176015A1

公开(公告)日：2015-06-25

申请号：US14416799

申请日：2013-07-23

Applicant: DOW AGROSCIENCES LLC

Inventor： Sandeep Kumar ,  Steven L. Evans ,  Manju Gupta

IPC: C12N15/82

CPC classification number: C12N15/8209 ,  C12N15/68 ,  C12N15/82

Abstract: This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.

Abstract translation: 本发明涉及用于转基因植物的载体组装的方法和系统。 使用统一的模块化方法来减少循环时间，本文提供的方法和系统可以使用多孔板（例如96孔板）提高克隆产量。 在一些实施方案中，本文提供的方法和系统消除或减少对测序确认的需要，因为在载体组装过程中不涉及PCR。

公开(公告)号：US10683513B2

公开(公告)日：2020-06-16

申请号：US14577887

申请日：2014-12-19

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Marcelo Ariel German ,  Tristan Coram ,  Terry R. Wright

IPC: C12N15/82

Abstract: This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in male plant tissues, for example, to provide an economical male sterility system of hybrid seed production.

公开(公告)号：US10519459B2

公开(公告)日：2019-12-31

申请号：US15698714

申请日：2017-09-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Pierluigi Barone ,  Daren Hemingway ,  Emily Etchison ,  Andrew Asberry ,  Heather Pence ,  Andrew J. Bowling

IPC: C12N15/82

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum (Pavir.J00490) egg cell gene. Some embodiments relate to a promoter from a Panicum virgatum (Pavir.J00490) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Panicum virgatum (Pavir.J00490) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US10400246B2

公开(公告)日：2019-09-03

申请号：US15698702

申请日：2017-09-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Pierluigi Barone ,  Daren Hemingway ,  Emily Etchison ,  Andrew Asberry ,  Heather Pence ,  Andrew J. Bowling

IPC: C12N15/82

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays egg cell gene. Some embodiments relate to a promoter from a Zea mays egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Zea mays egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US10400245B2

公开(公告)日：2019-09-03

申请号：US15485338

申请日：2017-04-12

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC: C12N15/82 ,  C12N15/00

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G047720 promoter. Some embodiments relate to a Zea mays GRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G047720 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US20190002910A1

公开(公告)日：2019-01-03

申请号：US15698708

申请日：2017-09-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Pierluigi Barone ,  Daren Hemingway ,  Emily Etchison ,  Andrew Asberry ,  Heather Pence ,  Andrew J. Bowling

IPC: C12N15/82 ,  C12Q1/6895

CPC classification number: C12N15/8274 ,  C12N15/8207 ,  C12N15/8216 ,  C12N15/8233 ,  C12N15/8234 ,  C12N15/8279 ,  C12N15/8286 ,  C12N2800/22 ,  C12N2820/65 ,  C12Q1/6895 ,  C12Q2600/13

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum (Pavir.Cb02009) egg cell gene. Some embodiments relate to a promoter from a Panicum virgatum (Pavir.Cb02009) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Panicum virgatum (Pavir.Cb02009) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US20190002905A9

公开(公告)日：2019-01-03

申请号：US14938632

申请日：2015-11-11

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Jeffrey R. Beringer ,  Wei Chen ,  Andrew M. Asberry

IPC: C12N15/82

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Zea mays Ubiquitin-1 gene promoter, and the full-length nucleotide sequence elements from a Rice Ubiquitin-3 promoter. Some embodiments relate to a synthetic bi-directional promoter that may function in plants to promote transcription of two operably linked nucleotide sequences.

公开(公告)号：US10030247B2

公开(公告)日：2018-07-24

申请号：US14587750

申请日：2014-12-31

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/82

CPC classification number: C12N15/8216

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. Hi-II.

公开(公告)号：US09988638B2

公开(公告)日：2018-06-05

申请号：US14938643

申请日：2015-11-11

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Toby Cicak ,  Heather Leigh Robinson ,  Dayakar Reddy Pareddy ,  Wei Chen

IPC: C12N15/82

CPC classification number: C12N15/8216

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Arabidopsis thaliana Ubiquitin-10 gene promoter or Cassava Vein Mosaic Virus promoter, and the full-length nucleotide sequence elements from a Cassava Vein Mosaic Virus promoter. Some embodiments relate to a synthetic CsVMV bi-directional promoter that functions in plants to promote transcription of two operably linked nucleotide sequences.