公开(公告)号：US06455310B1

公开(公告)日：2002-09-24

申请号：US09526006

申请日：2000-03-15

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: C12N500

CPC classification number: C12M23/10 ,  C12M23/22 ,  C12M23/24 ,  C12M25/02 ,  C12M47/04 ,  C12N13/00

Abstract: Provided is a cell culture apparatus for culturing cells, and optionally, for performing magnetic separation of cells desired to be cultured. The cell culture apparatus preferably comprises a frame; two membranes, preferably each being gas permeable, which are securedly sealed in a leak-proof selaing to a frame in forming a culture chamber therebetween; and at least one resealable aperture to allow substances to be introduced into, or withdrawn from, the culture chamber.

Abstract translation: 提供培养细胞的细胞培养装置，任选地，用于进行期望培养的细胞的磁分离。 细胞培养装置优选包括框架; 两个膜，优选各自是透气的，它们在形成其间的培养室的情况下被牢固地密封在防漏塞到框架中; 和至少一个可重新密封的孔，以允许物质被引入或从培养室中取出。

公开(公告)号：US06365124B1

公开(公告)日：2002-04-02

申请号：US09244592

申请日：1999-02-04

Applicant: Alvaro Babino ,  Eduardo Osinaga ,  Emilio Barbera-Guillem

Inventor： Alvaro Babino ,  Eduardo Osinaga ,  Emilio Barbera-Guillem

IPC: A61K5108

CPC classification number: G01N33/57484 ,  G01N33/57426

Abstract: Compositions are provided for detecting Tn antigen expressing-shed tumor antigen. The compositions include peptides having binding specificity for the Tn antigen; and a detector molecule comprising a peptide, according to the present invention, which is bound to a detectable label. The compositions may be used in a method to detect lymphoid tissues containing shed tumor antigen expressing the Tn antigen.

Abstract translation: 提供了用于检测表达Tn抗原的肿瘤抗原的组合物。 组合物包括对Tn抗原具有结合特异性的肽; 以及包含与可检测标记结合的根据本发明的肽的检测器分子。 组合物可用于检测含有表达Tn抗原的脱落肿瘤抗原的淋巴组织的方法。

公开(公告)号：US06319607B1

公开(公告)日：2001-11-20

申请号：US09590421

申请日：2000-06-08

Applicant: Emilio Barbera-Guillem ,  Stephanie L. Castro

Inventor： Emilio Barbera-Guillem ,  Stephanie L. Castro

IPC: A61K4900

CPC classification number: B82Y15/00 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  G01N33/588 ,  Y10T428/2989 ,  Y10T428/2993

Abstract: Provided are methods for purifying functionalized fluorescent nanocrystals having affinity ligand operably bound thereto by using a solid support matrix in a reactor through which solutions are circulated, and by using an immobilized solid phase formed by operably binding functionalized fluorescent nanocrystals to the solid support matrix. Also provided are purified functionalized fluorescent nanocrystals having affinity ligand operably bound thereto which are purified using the methods according to the present invention to be substantially free of free affinity ligand.

Abstract translation: 提供了通过在溶液循环的反应器中使用固体支持基质和通过使用通过可操作地将官能化荧光纳米晶体结合到固体支持基质上形成的固定化固相来纯化具有可操作地结合到其上的亲和配体的官能化荧光纳米晶体的方法。 还提供了具有可操作地结合到其上的亲和配体的纯化的官能化荧光纳米晶体，其使用根据本发明的方法纯化为基本上不含游离亲和配体。

公开(公告)号：US06309701B1

公开(公告)日：2001-10-30

申请号：US09577761

申请日：2000-05-24

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: A61K4900

CPC classification number: B82Y30/00 ,  B82Y5/00 ,  B82Y10/00 ,  Y10T428/2989 ,  Y10T428/2993

Abstract: Provided are a fluorescent microsphere comprised of a plurality of fluorescent nanocrystals operably bound to a polymeric microsphere, and a method of producing the fluorescent microspheres which comprises contacting the polymeric microsphere with a plurality of fluorescent nanocrystals under suitable conditions in which the fluorescent naocrystals become operably bound to the polymeric microsphere. Also provided is a method of using the fluorescent microspheres capable of determining the presence or absence of a predetermined number of analytes in a sample by contacting the sample with the fluorescent microspheres, and detecting the fluorescence signal pattern of excited fluorescent microspheres bound to one or more analytes of the predetermined number of analytes, if present in the sample.

Abstract translation: 提供了由可操作地结合到聚合物微球上的多个荧光纳米晶体组成的荧光微球，以及制备荧光微球的方法，其包括使聚合物微球与多个荧光纳米晶体在适当的条件下接触，其中荧光非晶体变得可操作地结合 到聚合物微球。 还提供了一种使用能够通过使样品与荧光微球接触来测定样品中预定数量的分析物的存在或不存在的荧光微球的方法，并且检测结合到一个或多个的激发的荧光微球的荧光信号图案 如果样品中存在预定数量的分析物的分析物。

公开(公告)号：US06268350B1

公开(公告)日：2001-07-31

申请号：US09479524

申请日：2000-01-07

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: A61K4800

CPC classification number: C12N15/1138 ,  A61K38/177 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/18

Abstract: Provided are one or more polynucleotides, and a method for inhibiting tumor progression in an individual comprising administering to the individual a therapeutically effective amount of the one or more polynucleotides. The one or more polynucleotides comprise FasL sense polynucleotide, or FasL antisense, or a combination thereof.

Abstract translation: 提供一种或多种多核苷酸，以及抑制个体中肿瘤进展的方法，其包括向个体施用治疗有效量的一种或多种多核苷酸。 一个或多个多核苷酸包含FasL有义多核苷酸或FasL反义，或其组合。

公开(公告)号：US06251616B1

公开(公告)日：2001-06-26

申请号：US09435289

申请日：1999-11-05

Applicant: Emilio Barbera-Guillem ,  M. Bud Nelson

Inventor： Emilio Barbera-Guillem ,  M. Bud Nelson

IPC: G01N33574

CPC classification number: G01N33/56972 ,  G01N2800/52 ,  Y10S435/975

Abstract: A method for screening for a pro-tumor immune response according to the present invention, the method comprises: contacting a clinical sample with one or more detector molecules for detecting, and then determining the amount of, mononuclear cell phenotype in the sample; and (b) comparing the amount of mononuclear cell phenotype determined in the sample to a reference value for the mononuclear cell phenotype; wherein a significant difference in the amount of mononuclear cell phenotype determined as compared to the reference value may be an indicator of the presence of a pro-tumor immune response. Also provided are assay kits for determining an amount of mononuclear cell phenotype in performing the methods according to the present invention.

Abstract translation: 根据本发明的用于筛选前肿瘤免疫应答的方法，所述方法包括：使临床样品与一个或多个检测分子接触，用于检测，然后确定样品中单核细胞表型的量; 和（b）将样品中测定的单核细胞表型的量与单核细胞表型的参考值进行比较; 其中与参考值相比确定的单核细胞表型的量的显着差异可以是前肿瘤免疫应答的存在的指标。 还提供了用于在执行根据本发明的方法中测定单核细胞表型的量的测定试剂盒。

公开(公告)号：US06179912B2

公开(公告)日：2001-01-30

申请号：US09468418

申请日：1999-12-20

Applicant: Emilio Barbera-Guillem ,  Marlin O. Thurston

Inventor： Emilio Barbera-Guillem ,  Marlin O. Thurston

IPC: C30B708

CPC classification number: B82Y30/00 ,  C01G1/02 ,  C01P2004/64 ,  C30B7/00 ,  C30B29/605 ,  Y10S977/813 ,  Y10S977/815 ,  Y10S977/835

Abstract: Provided is a system and continuous flow process for producing monodisperse semiconductor nanocrystals comprising reservoirs for the starting materials, a mixing path in which the starting materials are mixed, a first reactor in which the mixture of starting materials is mixed with a coordinating solvent and in which nucleation of particles occurs, a second reactor in which controlled growth of the nanocrystals occurs, and a growth termination path in which the growth of the nanocrystals is halted.

Abstract translation: 提供了一种用于生产单分散半导体纳米晶体的系统和连续流程方法，其包括用于起始材料的储存器，混合原料的混合路径，将起始材料混合物与配位溶剂混合的第一反应器，其中 发生粒子的成核，其中发生纳米晶体的受控生长的第二反应器和停止纳米晶体的生长的生长终止路径。

公开(公告)号：US6110380A

公开(公告)日：2000-08-29

申请号：US79469

申请日：1998-05-15

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: B01D35/06 ,  B03C1/00 ,  C12N13/00

CPC classification number: B03C1/01 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  Y10S435/803 ,  Y10S436/824

Abstract: Provided is a magnetic separation device comprising a container having one or more outer surfaces; at least one flexible magnetic sheet; and a non-permanent adhesive that is used to detachably secure an outer surface of the container to a flexible magnetic sheet. A method of using the magnetic separation device according to the present invention comprises obtaining a fluid containing a mixed population of biological molecules, from which it is desired to separate at least one subpopulation of biological molecules; mixing the fluid with a magnetic separation reagent; contacting the mixture with the fluid holding chamber of the magnetic separation device; incubating the mixture for a sufficient time to allow for complexes to form between the subpopulation of biological molecules and the magnetic separation reagent; positioning the magnetic separation device in a position that magnetically attracts the complexes towards the flexible magnetic sheet, and thereby holds them in position in the container; and removing the remainder of the fluid from the magnetic separation device.

Abstract translation: 提供一种磁分离装置，其包括具有一个或多个外表面的容器; 至少一个柔性磁性片; 以及用于将容器的外表面可拆卸地固定到柔性磁性片的非永久性粘合剂。 使用根据本发明的磁分离装置的方法包括获得含有生物分子的混合群体的流体，期望从其分离至少一个生物分子亚群; 将流体与磁性分离试剂混合; 使混合物与磁选机的流体保持室接触; 将混合物温育足够的时间以允许在生物分子的亚群与磁分离试剂之间形成复合物; 将磁分离装置定位在磁性吸引复合物朝向柔性磁性片的位置，从而将其保持在容器中的适当位置; 以及从磁分离装置中除去剩余的流体。

公开(公告)号：US5536642A

公开(公告)日：1996-07-16

申请号：US118969

申请日：1993-09-09

Applicant: Emilio Barbera-Guillem ,  Stefan A. Cohen

Inventor： Emilio Barbera-Guillem ,  Stefan A. Cohen

IPC: G01N33/48 ,  A61K39/395 ,  A61K47/48 ,  A61P35/00 ,  C12N5/06 ,  C12P21/08 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/574 ,  G01N33/68

CPC classification number: G01N33/6869 ,  A61K47/48423 ,  C12Q1/6886 ,  G01N33/5011 ,  G01N33/57488 ,  G01N33/57492 ,  C12Q2600/118 ,  G01N2333/55 ,  G01N2333/7051 ,  G01N2333/715 ,  G01N2800/52 ,  Y10S436/813

Abstract: The present invention is directed to the measurement of cell-associated interleukin-2 receptor .alpha. (IL-2R.alpha.) expression in solid non-lymphoid tumors, and the use of such measurement In prognosing the metastatic potential of the tumor, diagnosing the metastatic localization of non-lymphoid tumor, and aiding the monitoring of efficacy of anticancer therapy against metastatic cells of non-lymphoid tumor. The present invention also relates to the use of T-cell receptor (tumor specific TCR.beta. idiotype) in monitoring the efficacy of anticancer therapy against non-lymphoid tumors.

Abstract translation: 本发明涉及固体非淋巴样肿瘤中细胞相关白细胞介素-2受体α（IL-2Rα）表达的测量，以及这种测量的使用在预测肿瘤的转移潜能中，诊断转移定位 的非淋巴瘤肿瘤，并帮助监测抗癌治疗对非淋巴肿瘤转移细胞的疗效。 本发明还涉及T细胞受体（肿瘤特异性TCRβ独特型）在监测针对非淋巴样肿瘤的抗癌疗法的功效中的用途。

公开(公告)号：US08241765B2

公开(公告)日：2012-08-14

申请号：US12844816

申请日：2010-07-27

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: B32B9/00 ,  B32B19/00

CPC classification number: C09D11/50 ,  A61K9/167 ,  B82Y15/00 ,  C09K11/02 ,  C09K11/025 ,  C09K11/0805 ,  G07F7/086 ,  Y10T428/24802 ,  Y10T428/253

Abstract: A fluorescent ink composition comprising functionalized fluorescent nanocrystals, an aqueous-based ink carrier comprising water or a water-based solution, and a binder. Also provided are methods of providing an image or a security mark on a substrate for subsequent identification by applying a fluorescent ink composition onto the substrate.

Abstract translation: 包含官能化荧光纳米晶体的荧光墨水组合物，包含水或水基溶液的水基墨水载体和粘合剂。 还提供了在衬底上提供图像或安全标记以便随后通过将荧光墨水组合物施加到基底上的方法。