公开(公告)号：US6074823A

公开(公告)日：2000-06-13

申请号：US744590

申请日：1996-11-06

Applicant: Hubert Koster

Inventor： Hubert Koster

IPC: C07F9/24 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/68 ,  G01N35/10 ,  C12N9/16

CPC classification number: C07F9/2408 ,  C07F9/2429 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/6872 ,  G01N35/1067

Abstract: The invention provides fast and highly accurate mass spectrometer based processes for directly sequencing a target nucleic acid (or fragments generated from the target nucleic acid), which by means of protection, specificity of enzymatic activity, or immobilization, are unilaterally degraded in a stepwise manner via exonuclease digestion and the nucleotides, derivatives or truncated sequences detected by mass spectrometry.

公开(公告)号：US5851765A

公开(公告)日：1998-12-22

申请号：US454527

申请日：1995-05-30

Applicant: Hubert Koster

Inventor： Hubert Koster

IPC: G01N33/50 ,  C07F9/24 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N35/10 ,  C07H19/00 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/6872 ,  C07F9/2408 ,  C07F9/2429 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  G01N35/1067

Abstract: Methods for determining the sequence of nucleic acids by cleaving the nucleic acid unilaterally from a first end with an exonuclease activity to sequentially release individual nucleotides, identifying each of the sequentially release nucleotides by mass spectrometry, and determining the sequence of the nucleic acid from the identified nucleotides are disclosed. The method is amenable to multiplexing for simulataneously determining more than one nuleic acid sequence.